scholarly journals Characterization of a 3alpha-Hydroxysteroid Dehydrogenase/Carbonyl Reductase from the Gram-Negative Bacterium Comamonas testosteroni

1996 ◽  
Vol 241 (3) ◽  
pp. 744-749 ◽  
Author(s):  
Udo C. T. Oppermann ◽  
Edmund Maser
Keyword(s):  
Hydroxysteroid Dehydrogenase ◽  
Carbonyl Reductase ◽  
Comamonas Testosteroni ◽  
Negative Bacterium ◽  
Gram Negative

Characterization of 3,17β-hydroxysteroid dehydrogenase in Comamonas testosteroni

2015 ◽  
Vol 234 ◽  
pp. 221-228 ◽  
Author(s):  
Yuanhua Yu ◽  
Chuanzhi Liu ◽  
Baoxue Wang ◽  
Yanhong Li ◽  
Hao Zhang
Keyword(s):  
Hydroxysteroid Dehydrogenase ◽  
Comamonas Testosteroni

scholarly journals Identification and Biochemical Characterization of a Novel Protein Phosphatase 2C-Like Ser/Thr Phosphatase in Escherichia coli

2018 ◽  
Vol 200 (18) ◽  
Author(s):  
Krithika Rajagopalan ◽  
Elizabeth Nagle ◽  
Jonathan Dworkin
Keyword(s):  
Escherichia Coli ◽  
Protein Phosphatase ◽  
Biochemical Characterization ◽  
Regulatory Protein ◽  
Negative Bacterium ◽  
Gram Negative ◽  
Content Type ◽  
E Coli ◽  
Novel Protein

Regulatory protein phosphorylation is a conserved mechanism of signaling in all biological systems. Recent phosphoproteomic analyses of phylogenetically diverse bacteria, including the model Gram-negative bacteriumEscherichia coli, demonstrate that many proteins are phosphorylated on serine or threonine residues. In contrast to phosphorylation on histidine or aspartate residues, phosphorylation of serine and threonine residues is stable and requires the action of a partner Ser/Thr phosphatase to remove the modification. Although a number of Ser/Thr kinases have been reported inE. coli, no partner Ser/Thr phosphatases have been identified. Here, we biochemically characterize a novel Ser/Thr phosphatase that acts to dephosphorylate a Ser/Thr kinase that is encoded in the same operon.

scholarly journals Genetic characterization of Xylella fastidiosa isolated from citrus and coffee plants

2007 ◽  
Vol 64 (5) ◽  
pp. 482-485 ◽  
Author(s):  
Vicente Savonitti Miranda ◽  
Paulo Roberto Silva Farias ◽  
Sérgio Rufo Roberto ◽  
Pedro Magalhães Lacava
Keyword(s):  
Genetic Characterization ◽  
Xylella Fastidiosa ◽  
Random Amplified Polymorphic Dna ◽  
Negative Bacterium ◽  
Similarity Coefficients ◽  
Gram Negative ◽  
Coffee Plants ◽  
The Many ◽  
Group D

The Citrus Variegated Chlorosis and the Coffee Leaf Scorch are some of the many destructive diseases caused by Xylella fastidiosa, a gram-negative bacterium limited to the xylem of affected plants. As its genetic characterization is still not well established, different isolates of X. fastidiosa from citrus and coffee were evaluated through RAPD (Random Amplified Polymorphic DNA) technique to characterize and classify these isolates based on similarity coefficients. Sixteen isolates of X. fastidiosa were used on this trial, obtained from citrus, coffee and almond. The genetic polymorphism evaluation was performed using six arbitrary 10-base primer pairs. It was possible to establish a dendogram in which the isolates were classified into five groups (A, B, C, D and E). A prevalence of citrus isolates in groups A and D was observed. In groups B and C, there was a prevalence of coffee isolates meanwhile the group D consisted of the almond isolate, solely.

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