The phylogenetic position of East AsianSedumspecies (Crassulaceae) based on chloroplast DNA trnL (UAA)-trnF (GAA) intergenic spacer sequence variation

1996 ◽  
Vol 45 (3) ◽  
pp. 309-321 ◽  
Author(s):  
JEONG HEE KIM ◽  
HENK ‘T HART ◽  
TED H. M. MES
Keyword(s):  
Chloroplast Dna ◽  
Sequence Variation ◽  
Intergenic Spacer ◽  
Phylogenetic Position

Phylogenetic position ofKontumia(Polypodiaceae) inferred from four chloroplast DNA regions

2012 ◽  
pp. n/a-n/a
Author(s):  
Changkyun Kim ◽  
Hong-Guang Zha ◽  
Tao Deng ◽  
Hang Sun ◽  
Su-Gong Wu
Keyword(s):  
Chloroplast Dna ◽  
Phylogenetic Position ◽  
Chloroplast Dna Regions

scholarly journals Intraspecific Variation in Cannabis sativa L. Based on Intergenic Spacer Region of Chloroplast DNA.

2000 ◽  
Vol 23 (6) ◽  
pp. 727-730 ◽  
Author(s):  
Mareshige KOHJYOUMA ◽  
I-Jung LEE ◽  
Osamu IIDA ◽  
Kogo KURIHARA ◽  
Kazuya YAMADA ◽  
...  
Keyword(s):  
Chloroplast Dna ◽  
Intraspecific Variation ◽  
Cannabis Sativa ◽  
Intergenic Spacer ◽  
Intergenic Spacer Region

New insights into sequence variation in the IGS region of 21 cyathostomin species and the implication for molecular identification

Parasitology ◽  
2012 ◽  
Vol 139 (8) ◽  
pp. 1063-1073 ◽  
Author(s):  
K. CWIKLINSKI ◽  
F. N. J. KOOYMAN ◽  
D. C. K. VAN DOORN ◽  
J. B. MATTHEWS ◽  
J. E. HODGKINSON
Keyword(s):  
Comparative Analysis ◽  
Dna Sequences ◽  
Sequence Variation ◽  
Intergenic Spacer ◽  
Parasitic Nematodes ◽  
Sequence Information ◽  
Detailed Knowledge ◽  
Life Cycle Stages ◽  
Third Stage ◽  
Specific Variation

SUMMARYCyathostomins comprise a group of 50 species of parasitic nematodes that infect equids. Ribosomal DNA sequences, in particular the intergenic spacer (IGS) region, have been utilized via several methodologies to identify pre-parasitic stages of the commonest species that affect horses. These methods rely on the availability of accurate sequence information for each species, as well as detailed knowledge of the levels of intra- and inter-specific variation. Here, the IGS DNA region was amplified and sequenced from 10 cyathostomin species for which sequence was not previously available. Also, additional IGS DNA sequences were generated from individual worms of 8 species already studied. Comparative analysis of these sequences revealed a greater range of intra-specific variation than previously reported (up to 23%); whilst the level of inter-specific variation (3–62%) was similar to that identified in earlier studies. The reverse line blot (RLB) method has been used to exploit the cyathostomin IGS DNA region for species identification. Here, we report validation of novel and existing DNA probes for identification of cyathostomins using this method and highlight their application in differentiating life-cycle stages such as third-stage larvae that cannot be identified to species by morphological means†.

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