Molecular Phylogeny of Dipetrocarpaceae in Southeast Asia Based on Nucleotide Sequences ofmatK,trnL Intron, andtrnL-trnF Intergenic Spacer Region in Chloroplast DNA

1998 ◽  
Vol 10 (2) ◽  
pp. 202-209 ◽  
Author(s):  
Tadashi Kajita ◽  
Koichi Kamiya ◽  
Kaho Nakamura ◽  
Hidenori Tachida ◽  
Ratnam Wickneswari ◽  
...  
Keyword(s):  
Molecular Phylogeny ◽  
Southeast Asia ◽  
Chloroplast Dna ◽  
Intergenic Spacer ◽  
Nucleotide Sequences ◽  
Trnl Intron ◽  
Intergenic Spacer Region

scholarly journals Intraspecific Variation in Cannabis sativa L. Based on Intergenic Spacer Region of Chloroplast DNA.

2000 ◽  
Vol 23 (6) ◽  
pp. 727-730 ◽  
Author(s):  
Mareshige KOHJYOUMA ◽  
I-Jung LEE ◽  
Osamu IIDA ◽  
Kogo KURIHARA ◽  
Kazuya YAMADA ◽  
...  
Keyword(s):  
Chloroplast Dna ◽  
Intraspecific Variation ◽  
Cannabis Sativa ◽  
Intergenic Spacer ◽  
Intergenic Spacer Region

scholarly journals Associations between nuclear loci and chloroplast DNA genotypes in wild barley.

Genetics ◽  
1992 ◽  
Vol 131 (1) ◽  
pp. 225-231
Author(s):  
M A Maroof ◽  
Q Zhang ◽  
D B Neale ◽  
R W Allard
Keyword(s):  
Chloroplast Dna ◽  
Linear Models ◽  
Wild Barley ◽  
Blot Hybridization ◽  
Intergenic Spacer ◽  
Southern Blot Hybridization ◽  
Gene Pools ◽  
Intergenic Spacer Region ◽  
Evolutionary Forces ◽  
Nuclear Loci

Abstract Associations among alleles at nine nuclear loci and three chloroplast DNA (cpDNA) genotypes were assessed in a sample of 247 accessions of the wild barley, Hordeum vulgare ssp. spontaneum. Alleles at two of the nine nuclear loci are marked by length variations in the intergenic spacer region of ribosomal DNA (rDNA), and those of the other seven loci are well characterized allozymes. The three chloroplast DNA (cpDNA) genotypes are marked by restriction fragment length polymorphisms resulting from three polymorphic restriction sites detected by Southern blot hybridization. The analyses were performed by dividing the nine nuclear loci into a series of two-locus subsets and constructing log-linear models to characterize associations between the subsets of two nuclear loci and the cpDNA genotypes. Statistically significant associations were detected between six of the nine nuclear loci and the cpDNA genotypes, either individually as pairwise correlations, or through interaction with another nuclear locus to form three-variate complexes. Although the sample size of the present study was inadequate for statistical evaluation of higher order interactions, the results suggest the existence of interactions in which more than two nuclear loci are involved in associations with cpDNA genotypes. The observed cytonuclear associations appear to result from interplay among a number of evolutionary forces including a mating system of predominant selfing, differentiation among gene pools of local populations, and adaptation of barley genotypes to specific environmental conditions.

Molecular phylogeny ofNothofagus (Nothofagaceae) based on theatpB-rbcL intergenic spacer of the chloroplast DNA

1997 ◽  
Vol 110 (4) ◽  
pp. 469-484 ◽  
Author(s):  
Hiroaki Setoguchi ◽  
Mikio Ono ◽  
Yoshimichi Doi ◽  
Hiroshige Koyama ◽  
Mitsuya Tsuda
Keyword(s):  
Molecular Phylogeny ◽  
Chloroplast Dna ◽  
Intergenic Spacer

Phylogenetic analysis of Australian Acacia (Leguminosae: Mimosoideae) by using sequence variations of an intron and two intergenic spacers of chloroplast DNA

2000 ◽  
Vol 13 (5) ◽  
pp. 745 ◽  
Author(s):  
Daniel J. Murphy ◽  
Frank Udovicic ◽  
Pauline Y. Ladiges
Keyword(s):  
Phylogenetic Analysis ◽  
Chloroplast Dna ◽  
Small Sample Size ◽  
Intergenic Spacer ◽  
Small Sample ◽  
Total Evidence ◽  
Trnl Intron ◽  
Intergenic Spacers ◽  
Sequence Variations ◽  
Jackknife Support

Three regions of chloroplast DNA are assessed for their utility for phylogenetic analysis of Acacia subgenus Phyllodineae: psbA–trnH intergenic spacer, the trnL intron and the trnL–trnF intergenic spacer. There are large differences in the lengths of the psbA–trnH spacer (155–440 bp) and trnL–trnF intergenic spacer (101–422 bp) regions, and large multi-residue indels were coded as multistate characters. Overall information content in these regions is relatively low, but the total evidence tree has 12 nodes resolved, five with jackknife support. By using Parkia timoriana as the outgroup, Acacia subgenus Acacia (A. farnesiana) is basal and Acacia subgenus Aculeiferum (A. senegal) is the sister taxon to subgenus Phyllodineae. Although based on a small sample size, within subgenus Phyllodineae, the results of this study have shown that section Alatae is not monophyletic, section Lycopodiifoliae is monophyletic and Botrycephalae is related to members of section Phyllodineae with racemose inflorescences.

Regional Differences in Plant Development, Water Relations, and Chloroplast DNA of Hard Maples

HortScience ◽  
1998 ◽  
Vol 33 (3) ◽  
pp. 456d-456
Author(s):  
Rolston St. Hilaire ◽  
William R. Graves
Keyword(s):  
Chloroplast Dna ◽  
Water Relations ◽  
Plant Development ◽  
Regional Differences ◽  
Intergenic Spacer ◽  
Principal Component ◽  
Western Region ◽  
Intergenic Spacer Region ◽  
Geographical Regions ◽  
Leaf Osmotic Potential

Principal component analysis of foliar traits of hard maples (Acer saccharum Marsh. and Acer nigrum Michx. f.) near 43°N latitude clustered data into two populations composed of trees from different geographical regions. Seedlings from these two regions, and a third, geographically intermediate region, were grown in a greenhouse for 2 years with two irrigation frequencies to assess regional differences in plant development and water relations. Leaves from the most western region (west of 93°W longitude) had the highest specific mass (5.97 mg/cm2), trichome frequency (531/cm2), and stomate frequency (628/cm2). Across regions, plants irrigated frequently had more stomates (596/cm2) than plants irrigated sparsely (483/cm2). Traits similar across regions but higher with frequent irrigation included surface area and mass of lamina, shoot-to-root ratio, the ratio of lamina area to stem xylem diameter, and leaf water potential. Sparse irrigation caused a comparatively large decrease in stomatal conductance of plants from the most western region, and pressure-volume analysis showed no regional or irrigation effects on leaf osmotic potential at full turgor. Identical banding patterns resulted when Hinf I digested the PCR-amplified trnL-trnF intergenic spacer region of chloroplast DNA from each region; work with the rpL16 and ndhA introns is proceeding. Trichome frequency on abaxial leaf surfaces, which differs regionally both in nature and in controlled environments, is the most consistent character we have measured for discerning populations.

scholarly journals Differentiation of Two Biovars of Ureaplasma urealyticum Based on the 16S-23S rRNA Intergenic Spacer Region

1999 ◽  
Vol 37 (12) ◽  
pp. 4135-4138 ◽  
Author(s):  
Ryô Harasawa ◽  
Yasuo Kanamoto
Keyword(s):  
Nucleotide Sequence ◽  
Genetic Differentiation ◽  
Restriction Endonuclease ◽  
Ureaplasma Urealyticum ◽  
Intergenic Spacer ◽  
Nucleotide Sequences ◽  
23S Rrna ◽  
Trna Genes ◽  
Intergenic Spacer Region ◽  
Restriction Sites

The 16S-23S rRNA intergenic spacer regions of 14 strains representing the 14 serovars of Ureaplasma urealyticum were amplified by PCR and sequenced for genetic differentiation between the two biovars Parvo and T960. Although the spacer region of the Parvo and T960 biovars comprised 302 nucleotides and lacked spacer tRNA genes, 15 nucleotides were different between the two biovars. The four nucleotide sequences of the 16S-23S rRNA intergenic spacer region of serovars 1, 3, 6, and 14 in the Parvo biovar were found to be identical. Similarly, the 10 nucleotide sequences of the 16S-23S rRNA intergenic spacer region of serovars 2, 4, 5, and 7 to 13 in the T960 biovar were found to be identical. The nucleotide sequence of the T960 biovar contains multiple restriction sites for restriction endonucleaseSspI, which allows differentiation of the T960 biovar from the Parvo biovar.

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