Transcriptional differences between the male-sterile mutant bcms and wild-type Brassica campestris ssp. chinensis reveal genes related to pollen development

Five new recessive male-sterile mutants of Arabidopsis thaliana were isolated following seed mutagenesis by X-rays and ethyl methanesulfonate. The cytology of plants homozygous for the msY and msW mutations suggested that pollen development in these lines became abnormal at or before meiosis. The msK mutation caused faulty timing of synthesis or turnover and distribution of callose. In plants homozygous for the msZ mutation, pollen development failed at a late stage. In wild-type plants, the stamen filament elongated just prior to anther dehiscence. In contrast, in the msZ mutant stamen elongation did not occur. Pollen in msH homozygotes was fertile, but anthers failed to dehisce. The msI mutant of J.H. Van der Ween and P. Wirtz (1968. Euphytica 17: 371 – 377) was included in the present study. Pollen development in this mutant failed shortly after microspore release from tetrads. Complementation tests confirmed that the ms mutations were at different loci. Reduced transmission of certain ms genes was observed. Key words: Arabidopsis thaliana, male sterile mutants, anther dehiscence, callose, inheritance.

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Data for proteomic profiling of Anthers from a photosensitive male sterile mutant and wild-type cotton ( Gossypium hirsutum L.)

Data in Brief ◽

10.1016/j.dib.2015.06.022 ◽

2015 ◽

Vol 4 ◽

pp. 500-509 ◽

Cited By ~ 4

Author(s):

Ji Liu ◽

Chaoyou Pang ◽

Hengling Wei ◽

Meizhen Song ◽

Yanyan Meng ◽

...

Keyword(s):

Gossypium Hirsutum ◽

Proteomic Profiling ◽

Wild Type ◽

Male Sterile ◽

Gossypium Hirsutum L ◽

Sterile Mutant

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Morphological characteristics and transcriptome analysis at different anther development stages of the male sterile mutant MS7–2 in Wucai (Brassica campestris L.)

BMC Genomics ◽

10.1186/s12864-021-07985-5 ◽

2021 ◽

Vol 22 (1) ◽

Author(s):

Jian Wang ◽

Yitao Yang ◽

Lei Zhang ◽

Shaoxing Wang ◽

Lingyun Yuan ◽

...

Keyword(s):

Male Sterility ◽

Brassica Campestris ◽

Morphological Characteristics ◽

Development Stage ◽

Phenylpropanoid Metabolism ◽

Male Sterile ◽

Pollen Abortion ◽

Flower Buds ◽

Flower Bud ◽

Sterile Mutant

Abstract Background The discovery of male sterile materials is of great significance for the development of plant fertility research. Wucai (Brassica campestris L. ssp. chinensis var. rosularis Tsen) is a variety of non-heading Chinese cabbage. There are few studies on the male sterility of wucai, and the mechanism of male sterility is not clear. In this study, the male sterile mutant MS7–2 and the wild-type fertile plant MF7–2 were studied. Results Phenotypic characteristics and cytological analysis showed that MS7–2 abortion occurred at the tetrad period. The content of related sugars in the flower buds of MS7–2 was significantly lower than that of MF7–2, and a large amount of reactive oxygen species (ROS) was accumulated. Through transcriptome sequencing of MS7–2 and MF7–2 flower buds at three different developmental stages (a–c), 2865, 3847, and 4981 differentially expressed genes were identified in MS7–2 at the flower bud development stage, stage c, and stage e, respectively, compared with MF7–2. Many of these genes were enriched in carbohydrate metabolism, phenylpropanoid metabolism, and oxidative phosphorylation, and most of them were down-regulated in MS7–2. The down-regulation of genes involved in carbohydrate and secondary metabolite synthesis as well as the accumulation of ROS in MS7–2 led to pollen abortion in MS7–2. Conclusions This study helps elucidate the mechanism of anther abortion in wucai, providing a basis for further research on the molecular regulatory mechanisms of male sterility and the screening and cloning of key genes in wucai.

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