Histopathology of Susceptible and Resistant Capsicum annuum Cultivars Infected with Ralstonia solanacearum

1999 ◽  
Vol 147 (3) ◽  
pp. 129-140 ◽  
Author(s):  
M. A. Rahman ◽  
H. Abdullah ◽  
M. Vanhaecke
Keyword(s):  
Capsicum Annuum ◽  
Ralstonia Solanacearum

scholarly journals Identification of a Major QTL (qRRs-10.1) That Confers Resistance to Ralstonia solanacearum in Pepper (Capsicum annuum) Using SLAF-BSA and QTL Mapping

2019 ◽  
Vol 20 (23) ◽  
pp. 5887 ◽  
Author(s):  
Heshan Du ◽  
Changlong Wen ◽  
Xiaofen Zhang ◽  
Xiulan Xu ◽  
Jingjing Yang ◽  
...  
Keyword(s):  
Qtl Mapping ◽  
Capsicum Annuum ◽  
Ralstonia Solanacearum ◽  
Bulked Segregant Analysis ◽  
Severity Index ◽  
Clear Cut ◽  
Close Proximity ◽  
Specific Locus ◽  
Resistance Markers ◽  
Resistant Rootstock

The soilborne pathogen Ralstonia solanacearum is the causal agent of bacterial wilt (BW), a major disease of pepper (Capsicum annuum). The genetic basis of resistance to this disease in pepper is not well known. This study aimed to identify BW resistance markers in pepper. Analysis of the dynamics of bioluminescent R. solanacearum colonization in reciprocal grafts of a resistant (BVRC 1) line and a susceptible (BVRC 25) line revealed that the resistant rootstock effectively suppressed the spreading of bacteria into the scion. The two clear-cut phenotypic distributions of the disease severity index in 440 F2 plants derived from BVRC 25 × BVRC 1 indicated that a major genetic factor as well as a few minor factors that control BW resistance. By specific-locus amplified fragment sequencing combined with bulked segregant analysis, two adjacent resistance-associated regions on chromosome 10 were identified. Quantitative trait (QTL) mapping revealed that these two regions belong to a single QTL, qRRs-10.1. The marker ID10-194305124, which reached a maximum log-likelihood value at 9.79 and accounted for 19.01% of the phenotypic variation, was located the closest to the QTL peak. A cluster of five predicted R genes and three defense-related genes, which are located in close proximity to the significant markers ID10-194305124 or ID10-196208712, are important candidate genes that may confer BW resistance in pepper.

scholarly journals Isolation and Characterization of Wilt-Causing Pathogens of Local Growing Pepper (Capsicum annuum L.) in Gurage Zone, Ethiopia

2020 ◽  
Vol 2020 ◽  
pp. 1-8
Author(s):  
Temesgen Oljira ◽  
Sefawdin Berta
Keyword(s):  
Fusarium Oxysporum ◽  
Capsicum Annuum ◽  
Ralstonia Solanacearum ◽  
Disease Assessment ◽  
Management Approach ◽  
Pathogenicity Test ◽  
Biochemical Tests ◽  
Capsicum Annuum L ◽  
Local Cultivar ◽  
Isolation And Characterization

The yield of pepper (Capsicum annuum L.) is extremely threatened by different diseases in Ethiopia. The objective of the study was isolation of wilt-causing pathogens and susceptibility test of local growing pepper. Eighteen pepper farming fields were selected for disease assessment study. The samples of Mareko Fana, Dubi, and Mitmita local cultivar pepper’s pods, seeds, leaves, stems, and roots were collected, surface sterilized, and cultured on potato dextrose agar (PDA). Selective peptone pentachloronitrobenzene (PCNB) agar medium was used for fungus. Similarly, for bacteria isolation, nutrient agar (NA) was used. Morphological and biochemical tests revealed eleven fungal isolates of Fusarium oxysporum f. sp. that were isolated. The pathogenicity test confirmed nine of them were virulent to Mareko Fana, Dubi, and Mitmita local pepper. It is confirmed that Fusarium oxysporum f. sp. is the pathogen Fusarium oxysporum f. sp. capsici. Besides, Ralstonia solanacearum was identified as a bacterium pathogen causing complex pepper wilt disease. The highest mean PDI was registered in Remuga Keble (93.0%) and the lowest in Buyi Keble (58.3%). Similarly, the highest mean PSI was recorded in Buyi Keble (87.0%) and the lowest PSI (54.5%) was registered in Tawlla Keble. Among 60 seeds, Mareko local pepper inoculated by F. oxysporum f. sp. and R. solanacearum shows the highest susceptibility of 55 (91.0%) and 30 (50.0%), respectively. However, Mitmita local pepper was registered as the lowest susceptibility to both F. oxysporum f. sp. and R. solanacearum of 28.3% and 30.0%, respectively. Based on the finding, it can be concluded that pepper wilt was caused by a complex of fungus Fusarium oxysporum f. sp. capsici and bacteria Ralstonia solanacearum sp. in the study area. So, it is recommended that an integrated disease management approach should be implemented to manage the complex diseases of the site.

scholarly journals Murcha bacteriana no estado do Amazonas, Brasil

2004 ◽  
Vol 29 (1) ◽  
pp. 17-23 ◽  
Author(s):  
Rosalee A. Coelho Netto ◽  
Bianca G Pereira ◽  
Hiroshi Noda ◽  
Bernard Boher
Keyword(s):  
Lycopersicon Esculentum ◽  
Capsicum Annuum ◽  
Cucumis Sativus ◽  
Ralstonia Solanacearum ◽  
Moringa Oleifera ◽  
Solanum Melongena ◽  
Musa Spp ◽  
Musa Sp

Durante 1998 e 2000, a incidência de murcha bacteriana causada por Ralstonia solanacearum foi registrada em 25 municípios do estado do Amazonas. A bactéria foi encontrada nas seguintes espécies vegetais: Capsicum annuum, C. chinense, C. frutescens, Cucumis sativus, Heliconia sp., Lycopersicon esculentum, Melanthera discoidea, Moringa oleifera, Musa sp., Solanum melongena, S. gilo, e S. nigrum. Em tomateiros (Lycopersicon esculentum), a murcha bacteriana estava presente em todos os plantios. Em bananeiras (Musa spp.), a incidência do Moko foi menor nas várzeas dos rios Madeira e Negro do que nas dos rios Solimões e Amazonas. Caracterizaram-se 320 isolados de R. solanacearum, obtidos no levantamento, com relação a raça e a biovar. A biovar 1 predominou em todos os hospedeiros, com exceção de C. annuum e C. chinense, onde estirpes da biovar 3 foram maioria. Apenas 7,8% das estirpes foram da biovar N2. A sensibilidade de 56 estirpes da raça 1 a 23 bacteriocinas foi avaliada. As estirpes da biovar 3 apresentaram uma menor variabilidade, na sensibilidade a bacteriocinas do que as estirpes das biovares 1 e N2.

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