Strain Differentiation of Clostridium perfringens by Bacteriocin Typing, Plasmid Profiling and Ribotyping

SUMMARYA collection of 50 bacteriocins was assembled and used to type 802 isolates ofClostridium perfringensfrom food poisoning outbreaks and a variety of other sources. It was found that strains of the same serotype within an outbreak showed similar patterns of susceptibility to bacteriocins, and the use of a ‘one difference’ rule is proposed for interpretation of the typing patterns of epidemiologically related strains. Isolates of different serotype or of the same serotype isolated from different sources produced many variations in bacteriocin susceptibility patterns.Two computer programs were developed to assist in the interpretation of bacteriocin typing patterns. Their use showed that related and unrelated strains formed different clusters and enabled a range of the 20 most discriminatory bacteriocins to be selected.Isolates ofC. perfringensfrom a wide range of sources were screened for their ability to produce bacteriocins. A much greater proportion of the strains from food poisoning outbreaks was bacteriocinogenic than were isolates from human and animal infections, various foods and the environment. The relevance of these findings to the occurrence ofC. perfringensfood poisoning is discussed.

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Use of plasmid profiling as a typing method for epidemiologically related Clostridium perfringens isolates from food poisoning cases and outbreaks

Letters in Applied Microbiology ◽

10.1111/j.1472-765x.1995.tb00448.x ◽

1995 ◽

Vol 20 (5) ◽

pp. 290-294 ◽

Cited By ~ 14

Author(s):

H. Eisgruber ◽

M. Wiedmann ◽

A. Stolle

Keyword(s):

Clostridium Perfringens ◽

Food Poisoning ◽

Typing Method ◽

Plasmid Profiling

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Study of Plasmid Profiling of Clostridium perfringens Isolated from Small Ruminants of Odisha

International Journal of Livestock Research ◽

10.5455/ijlr.20191126074300 ◽

2020 ◽

pp. 1

Author(s):

Niranjan Soren ◽

Rajashree Mishra ◽

Iswar Senapati ◽

Kailash Bisht ◽

Bidyut Mishra ◽

...

Keyword(s):

Clostridium Perfringens ◽

Small Ruminants ◽

Plasmid Profiling

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Bacteriocin typing of Clostridium perfringens in human feces

Journal of Clinical Microbiology ◽

10.1128/jcm.7.3.307-309.1978 ◽

1978 ◽

Vol 7 (3) ◽

pp. 307-309

Author(s):

D E Mahony ◽

C A Swantee

Keyword(s):

Clostridium Perfringens ◽

Human Feces ◽

Stool Specimens ◽

Bacteriocin Typing

Three hundred and ninety-nine isolates of Clostridium perfringens from enriched stool specimens of 51 individuals (about eight colonies per person) were typed by bacteriocins. Forty-nine percent of these persons carried more than one bacteriocin type in their stool, and some had three or four different stains as determined by bacteriocin typing. Weekly stool specimens obtained from seven positive volunteers over a period of 5 weeks were screened for C. perfringens, and several colonies from each person were typed. This survey demonstrated that the number of types fluctuated with time, several types could be carried simultaneously, and the isolation of the organism was variable. Nine new bacteriocin types of C. perfringens were isolated in this study.

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