scholarly journals Bacteriocin typing of Clostridium perfringens in human feces

1978 ◽  
Vol 7 (3) ◽  
pp. 307-309
Author(s):  
D E Mahony ◽  
C A Swantee
Keyword(s):  
Clostridium Perfringens ◽  
Human Feces ◽  
Stool Specimens ◽  
Bacteriocin Typing

Three hundred and ninety-nine isolates of Clostridium perfringens from enriched stool specimens of 51 individuals (about eight colonies per person) were typed by bacteriocins. Forty-nine percent of these persons carried more than one bacteriocin type in their stool, and some had three or four different stains as determined by bacteriocin typing. Weekly stool specimens obtained from seven positive volunteers over a period of 5 weeks were screened for C. perfringens, and several colonies from each person were typed. This survey demonstrated that the number of types fluctuated with time, several types could be carried simultaneously, and the isolation of the organism was variable. Nine new bacteriocin types of C. perfringens were isolated in this study.

scholarly journals The assessment and application of a bacteriocin typing scheme forClostridium perfringens

1985 ◽  
Vol 94 (1) ◽  
pp. 69-79 ◽  
Author(s):  
G. N. Watson
Keyword(s):  
Clostridium Perfringens ◽  
Food Poisoning ◽  
Computer Programs ◽  
Typing Scheme ◽  
Wide Range ◽  
Bacteriocin Typing ◽  
Different Sources ◽  
Susceptibility Patterns

SUMMARYA collection of 50 bacteriocins was assembled and used to type 802 isolates ofClostridium perfringensfrom food poisoning outbreaks and a variety of other sources. It was found that strains of the same serotype within an outbreak showed similar patterns of susceptibility to bacteriocins, and the use of a ‘one difference’ rule is proposed for interpretation of the typing patterns of epidemiologically related strains. Isolates of different serotype or of the same serotype isolated from different sources produced many variations in bacteriocin susceptibility patterns.Two computer programs were developed to assist in the interpretation of bacteriocin typing patterns. Their use showed that related and unrelated strains formed different clusters and enabled a range of the 20 most discriminatory bacteriocins to be selected.Isolates ofC. perfringensfrom a wide range of sources were screened for their ability to produce bacteriocins. A much greater proportion of the strains from food poisoning outbreaks was bacteriocinogenic than were isolates from human and animal infections, various foods and the environment. The relevance of these findings to the occurrence ofC. perfringensfood poisoning is discussed.

scholarly journals Cytometric Approach for Detection of Encephalitozoon intestinalis, an Emergent Agent

2009 ◽  
Vol 16 (7) ◽  
pp. 1021-1024 ◽  
Author(s):  
Joana Barbosa ◽  
Acácio Gonçalves Rodrigues ◽  
Cidália Pina-Vaz
Keyword(s):  
Tap Water ◽  
Human Feces ◽  
Specific Flow ◽  
New Approach ◽  
Encephalitozoon Intestinalis ◽  
Flow Cytometric ◽  
Small Spore ◽  
Stool Specimens ◽  
Treatment Procedures ◽  
Infectious Form

ABSTRACT Encephalitozoon intestinalis is responsible for intestinal disease in patients with AIDS and immunocompetent patients. The infectious form is a small spore that is resistant to water treatment procedures. Its detection is very important, but detection is very cumbersome and time-consuming. Our main objective was to develop and optimize a specific flow cytometric (FC) protocol for the detection of E. intestinalis in hospital tap water and human feces. To determine the optimal specific antibody (Microspor-FA) concentration, a known concentration of E. intestinalis spores (Waterborne, Inc.) was suspended in hospital tap water and stool specimens with different concentrations of Microspor-FA, and the tap water and stool specimens were incubated under different conditions. The sensitivity limit and specificity were also evaluated. To study spore infectivity, double staining with propidium iodide (PI) and Microspor-FA was undertaken. Distinct approaches for filtration and centrifugation of the stool specimens were used. E. intestinalis spores stained with 10 μg/ml of Microspor-FA at 25°C overnight provided the best results. The detection limit was 5 × 104 spores/ml, and good specificity was demonstrated. Simultaneous staining with Microspor-FA and PI ensured that the E. intestinalis spores were dead and therefore noninfectious. With the stool specimens, better spore recovery was observed with a saturated solution of NaCl and centrifugation at 1,500 × g for 15 min. A new approach for the detection of E. intestinalis from tap water or human feces that ensures that the spores are not viable is now available and represents an important step for the prevention of this threat to public health.

Competitive erythroimmunoassay for detecting Clostridium perfringens type a enterotoxin in stool specimens

1990 ◽  
Vol 141 (5) ◽  
pp. 563-571 ◽  
Author(s):  
Y Germani ◽  
M.R Popoff ◽  
E Bégaud ◽  
J.L Guesdon
Keyword(s):  
Clostridium Perfringens ◽  
Type A ◽  
Stool Specimens

Foodborne Illness Associated with a Pig Roast

1987 ◽  
Vol 50 (11) ◽  
pp. 945-947
Author(s):  
THOMAS NOVOTNY ◽  
JAMES D. DINGMAN ◽  
ANDREW PAVIA
Keyword(s):  
Clostridium Perfringens ◽  
Foodborne Illness ◽  
Fisher Exact Test ◽  
Exact Test ◽  
Environmental Investigation ◽  
Foodborne Outbreaks ◽  
Stool Specimens

Roasting of whole pigs for summer picnics is popular, but technically difficult. We report an outbreak of gastroenteritis which followed a pig roast in Colorado. Twenty (35%) of 57 guests who had attended the roast had nausea, diarrhea, abdominal cramps, vomiting, or fever. An investigation implicated pork as the vehicle of transmission (p = 0.003, Fisher exact test). The 11 stool specimens tested were not cultured anaerobically, but the illness was strongly suggestive of Clostridium perfringens gastroenteritis. An environmental investigation revealed deficiencies in both storage and cooking of the commercially prepared pig. To prevent foodborne outbreaks of illness resulting from whole pig roasts, suppliers should caution customers about adequate cooking processes, customers should be aware of refrigeration requirements if the animal is to be stored before cooking, meat thermometers should be used to monitor internal cooking temperatures, other food should not be cooked inside the pig carcass during roasting, and leftover meat should be promptly cooled for later consumption.

scholarly journals The potential of bacteriocin typing in the study of Clostridium perfringens food poisoning.

1982 ◽  
Vol 35 (12) ◽  
pp. 1361-1365 ◽  
Author(s):  
G N Watson ◽  
M F Stringer ◽  
R J Gilbert ◽  
D E Mahony
Keyword(s):  
Clostridium Perfringens ◽  
Food Poisoning ◽  
Bacteriocin Typing
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