Isolation, Identification and Characterization of Group A Rotavirus from a Chicken: the Inner Capsid Protein Sequence shows only a Distant Phylogenetic Relationship to Most Other Avian Group A Rotaviruses

2005 ◽  
Vol 52 (5) ◽  
pp. 211-213 ◽  
Author(s):  
M. Elschner ◽  
H. Hotzel ◽  
J. Reetz ◽  
R. Diller ◽  
P. Otto
Keyword(s):  
Capsid Protein ◽  
Phylogenetic Relationship ◽  
Protein Sequence ◽  
Capsid Protein Sequence ◽  
Group A Rotavirus ◽  
Group A Rotaviruses ◽  
Group A ◽  
Avian Group ◽  
Identification And Characterization

scholarly journals Molecular Characterization of a Subgroup Specificity Associated with the Rotavirus Inner Capsid Protein VP2

2008 ◽  
Vol 82 (6) ◽  
pp. 2752-2764 ◽  
Author(s):  
Sarah M. McDonald ◽  
John T. Patton
Keyword(s):  
Monoclonal Antibody ◽  
Capsid Protein ◽  
Neutralizing Antibodies ◽  
Bovine Rotavirus ◽  
Single Region ◽  
Molecular Determinants ◽  
High Resolution Structure ◽  
Group A Rotaviruses ◽  
Group A

ABSTRACT Group A rotaviruses are classified into serotypes, based on the reactivity pattern of neutralizing antibodies to VP4 and VP7, as well as into subgroups (SGs), based on non-neutralizing antibodies directed against VP6. The inner capsid protein (VP2) has also been described as a SG antigen; however, little is known regarding the molecular determinants of VP2 SG specificity. In this study, we characterize VP2 SGs by correlating genetic markers with the immunoreactivity of the SG-specific monoclonal antibody (YO-60). Our results show that VP2 proteins similar in sequence to that of the prototypic human strain Wa are recognized by YO-60, classifying them as VP2 SG-II. In contrast, proteins not bound by YO-60 are similar to those of human strains DS-1 or AU-1 and represent VP2 SG-I. Using a mutagenesis approach, we identified residues that determine recognition by either YO-60 or the group A-specific VP2 monoclonal antibody (6E8). We found that YO-60 binds to a conformationally dependent epitope that includes Wa VP2 residue M328. The epitope for 6E8 is also contingent upon VP2 conformation and resides within a single region of the protein (Wa VP2 residues A440 to T530). Using a high-resolution structure of bovine rotavirus double-layered particles, we predicted these epitopes to be spatially distinct from each other and located on opposite surfaces of VP2. This study reveals the extent of genetic variation among group A rotavirus VP2 proteins and illuminates the molecular basis for a previously described SG specificity associated with the rotavirus inner capsid protein.

scholarly journals Molecular characterization of the major capsid protein VP6 of bovine group B rotavirus and its use in seroepidemiology

2005 ◽  
Vol 86 (9) ◽  
pp. 2569-2575 ◽  
Author(s):  
Hiroshi Tsunemitsu ◽  
Mariko Kamiyama ◽  
Kenji Kawashima ◽  
Ken Katsuda ◽  
Mariko Kohmoto ◽  
...  
Keyword(s):  
Capsid Protein ◽  
Expression System ◽  
Baculovirus Expression System ◽  
Reading Frame ◽  
High Prevalence ◽  
Group A Rotaviruses ◽  
Group A ◽  
Vp6 Gene ◽  
Group B

The major inner capsid protein (VP6) gene of the bovine group B rotavirus (GBR) Nemuro strain is 1269 nt in length and contains one open reading frame encoding 391 aa. Nucleotide and amino acid sequence identities of the Nemuro VP6 gene compared with the published corresponding human and rodent GBR genes were respectively 66–67 and 70–72 %, which are notably lower than those between human and rodent viruses (72–73 and 83–84 %, respectively). Overall identities of VP6 genes among GBRs were substantially lower than those among both group A rotaviruses (GARs) and group C rotaviruses (GCRs) derived from different species of mammals. These results demonstrate that bovine GBR is remarkably distinct from other GBRs and that GBRs from different species may have had a longer period of divergence than GARs and GCRs. Recombinant VP6 was generated with a baculovirus expression system and used for an ELISA to detect GBR antibodies. All 13 paired sera from adult cows with GBR-induced diarrhoea in the field showed antibody responses in the ELISA. In serological surveys of GBR infection using the ELISA, 47 % of cattle sera were positive for GBR antibodies, with a higher antibody prevalence in adults than in young cattle. In pigs, a high prevalence of GBR antibodies (97 %) was detected in sera from sows. These results suggest that GBR infection is common in cattle and pigs, notwithstanding the scarcity of reports of GBR detection in these species to date.

Characterization of a Porcine Enterocyte Receptor for Group a Rotavirus

1997 ◽  
pp. 135-143 ◽  
Author(s):  
Mark S. Kuhlenschmidt ◽  
Mark D. Rolsma ◽  
Theresa B. Kuhlenschmidt ◽  
Howard B. Gelberg
Keyword(s):  
Group A Rotavirus ◽  
Group A

scholarly journals Characterization of novel VP7, VP4, and VP6 genotypes of a previously untypeable group A rotavirus

Virology ◽  
2009 ◽  
Vol 385 (1) ◽  
pp. 58-67 ◽  
Author(s):  
Owen D. Solberg ◽  
Maria Eloisa Hasing ◽  
Gabriel Trueba ◽  
Joseph N.S. Eisenberg
Keyword(s):  
Group A Rotavirus ◽  
Group A

Identification and characterization of a novel capsid protein encoded by Singapore grouper iridovirus ORF038L

2010 ◽  
Vol 155 (3) ◽  
pp. 351-359 ◽  
Author(s):  
Qing Jiao Wan ◽  
Jie Gong ◽  
Xiao Hong Huang ◽  
You Hua Huang ◽  
Sheng Zhou ◽  
...  
Keyword(s):  
Capsid Protein ◽  
Identification And Characterization

scholarly journals Revised Mimivirus major capsid protein sequence reveals intron-containing gene structure and extra domain

2009 ◽  
Vol 10 (1) ◽  
pp. 39 ◽  
Author(s):  
Saïd Azza ◽  
Christian Cambillau ◽  
Didier Raoult ◽  
Marie Suzan-Monti
Keyword(s):  
Capsid Protein ◽  
Gene Structure ◽  
Protein Sequence ◽  
Major Capsid Protein ◽  
Capsid Protein Sequence

Molecular characterization of the first human G15 rotavirus strain of zoonotic origin from the bovine species

2021 ◽  
Vol 102 (4) ◽  
Author(s):  
Takeshi Tsugawa ◽  
Yoshiki Fujii ◽  
Yusuke Akane ◽  
Saho Honjo ◽  
Kenji Kondo ◽  
...  
Keyword(s):  
Phylogenetic Analysis ◽  
Molecular Characterization ◽  
Human Infection ◽  
Potential Reservoir ◽  
Group A Rotaviruses ◽  
Paediatric Outpatient ◽  
Group A ◽  
Human Infections ◽  
Bovine Species

Group A rotaviruses (RVAs) infect a wide variety of mammalian and avian species. Animals act as a potential reservoir to RVA human infections by direct virion transmission or by contributing genes to reassortants. Here, we report the molecular characterization of a rare human RVA strain Ni17-46 with a genotype G15P[14], isolated in Japan in 2017 during rotavirus surveillance in a paediatric outpatient clinic. The genome constellation of this strain was G15-P[14]-I2-R2-C2-M2-A13-N2-T9-E2-H3. This is the first report of an RVA with G15 genotype in humans, and sequencing and phylogenetic analysis results suggest that human infection with this strain has zoonotic origin from the bovine species. Given the fact that this strain was isolated from a patient with gastroenteritis and dehydration symptoms, we must take into account the virulence of this strain in humans.

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