DECREASED AMPA GLuR2, BUT NOT GLuR3, mRNA EXPRESSION IN RAT AMYGDALA AND DORSAL HIPPOCAMPUS FOLLOWING MORPHINE-INDUCED BEHAVIOURAL SENSITIZATION

The contribution of Interleukin-1β (IL-1β) to neuronal injury induced by status epilepticus (SE) in the immature brain remains unclear. The goal of this study was to determine the hippocampal expression of IL-1β and its type 1 receptor (IL-1RI) following SE induced by the lithium-pilocarpine model in fourteen-days-old rat pups; control animals were given an equal volume of saline instead of the convulsant. IL-1β and IL-1RI mRNA hippocampal levels were assessed by qRT-PCR 6 and 24 h after SE or control conditions. IL-1β and IL-1RI expression was detected in the dorsal hippocampus by immunohistochemical procedures; Fluoro-Jade B staining was carried out in parallel sections in order to detect neuronal cell death. IL-1β mRNA expression was increased 6 h following SE, but not at 24 h; however IL-1RI mRNA expression was unaffected when comparing with the control group. IL-1β and IL-1RI immunoreactivity was not detected in control animals. IL-1β and IL-1RI were expressed in the CA1 pyramidal layer, the dentate gyrus granular layer and the hilus 6 h after SE, whereas injured cells were detected 24 h following seizures. Early expression of IL-1β and IL-1RI in the hippocampus could be associated with SE-induced neuronal cell death mechanisms in the developing rat.

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B67 INCREASE IN BASAL GAD67 mRNA EXPRESSION IN THE CENTRAL NUCLEUS OF THE AMYGDALA: A MARKER OF STRESS AND DRUG-INDUCED BEHAVIOURAL SENSITIZATION

Behavioural Pharmacology ◽

10.1097/00008877-200509001-00272 ◽

2005 ◽

Vol 16 (Supplement 1) ◽

pp. S87

Author(s):

C. Casal ◽

A.R. Carta ◽

C. Cadoni ◽

F. Pirlo ◽

G. Di Chiara

Keyword(s):

Mrna Expression ◽

Central Nucleus ◽

Drug Induced ◽

Behavioural Sensitization

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Changes in Proenkephalin mRNA expression in forebrain areas after amphetamine-induced behavioural sensitization

Pharmacology Biochemistry and Behavior ◽

10.1016/j.pbb.2007.04.019 ◽

2007 ◽

Vol 87 (2) ◽

pp. 232-240 ◽

Cited By ~ 5

Author(s):

Marcela Morales-Mulia ◽

Fany Panayi ◽

Laura Lambás-Señas ◽

Hélène Scarna ◽

Milagros Méndez

Keyword(s):

Mrna Expression ◽

Behavioural Sensitization ◽

Proenkephalin Mrna

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Endocannabinoid Brain System Involvement in dopamine mechanisms of behavioural sensitization to psychostimulants

Acta Veterinaria Brno ◽

10.2754/avb200978030491 ◽

2009 ◽

Vol 78 (3) ◽

pp. 491-496

Author(s):

Dagmar Vršková

Keyword(s):

Mrna Expression ◽

Endocannabinoid System ◽

Intravenous Drug ◽

Self Administration ◽

System Involvement ◽

Brain System ◽

Behavioural Sensitization

The paper deals with the involvement of endocannabionid brain system in dopamine mechanisms of behavioural sensitization to psychostimulants. The development and manifestation of behavioural sensitization to methamphetamine are described. Results obtained in our previous studies, showing an interaction between the endocannabinoid system and methamphetamine brain mechanisms, in the rat intravenous drug self-administration model and with qPCR CB1 mRNA expression, are discussed.

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Reference Gene Validation Within the Rat Brain Under Mild Intermittent Ketosis Induced by Supplementation with Medium-Chain Triglycerides

10.21203/rs.3.rs-851204/v1 ◽

2021 ◽

Author(s):

Alexander Pavlovich Schwarz ◽

Veronika Alexandrovna Nikitina ◽

Darya Uladzimirawna Krytskaya ◽

Ksenia Pavlovna Shcherbakova ◽

Alexander Nikolaevich Trofimov

Keyword(s):

Prefrontal Cortex ◽

Mrna Expression ◽

Medial Prefrontal Cortex ◽

Reference Gene ◽

Reference Genes ◽

Dorsal Hippocampus ◽

Housekeeping Genes ◽

Ventral Hippocampus ◽

Medium Chain Triglycerides ◽

Medium Chain

Abstract RT-qPCR has become the gold standard in mRNA expression analysis. However, it requires an accurate choice of reference genes for adequate normalization. This work aims to validate the reference genes for qPCR experiments in the brain of rats in the model of mild ketosis established through supplementation with medium-chain triglycerides (MCT) and intermittent fasting. The standard chow of adult Wistar rats was supplemented with MCT oil (2 ml/kg, i.g., during 6 h of fasting) or water (equivolume) for 1 month. The mRNA expression of 9 housekeeping genes (Actb, B2m, Gapdh, Hprt1, Pgk1, Ppia, Rpl13a, Sdha, Ywhaz) in the medial prefrontal cortex, dorsal and ventral hippocampus was measured by RT-qPCR. Using the RefFinder® online tool, we have found that the reference gene stability ranking strongly depended on the analyzed brain region. The most stably expressed reference genes were found to be Ppia, Actb, and Rpl13a in the medial prefrontal cortex; Rpl13a, Ywhaz, and Pgk1 in the dorsal hippocampus; Ywhaz, Sdha, and Ppia in the ventral hippocampus. The B2m was identified as an invalid reference gene in the ventral hippocampus, while Sdha, Actb, and Gapdh were unstable in the dorsal hippocampus. The stabilities of the examined housekeeping genes were lower in the dorsal hippocampus compared to the ventral hippocampus and the medial prefrontal cortex. Thus, the expression stability of reference genes strongly depends on the examined brain regions. The dorsal and ventral hippocampal areas differ in reference genes stability rankings, which should be taken into account in the RT-qPCR experimental design.

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