scholarly journals Oxygen-dependent K+fluxes in sheep red cells

1998 ◽  
Vol 506 (3) ◽  
pp. 679-688 ◽  
Author(s):  
E. H. Campbell ◽  
J. S. Gibson
Keyword(s):  
Red Cells ◽  
Sheep Red Cells

Receptors for IgG molecules on human lymphocytes forming spontaneous rosettes with sheep red cells

1975 ◽  
Vol 5 (1) ◽  
pp. 70-72 ◽  
Author(s):  
M. Ferrarini ◽  
L. Moretta ◽  
Roberte Abrile ◽  
Maria Laura Durnte
Keyword(s):  
Human Lymphocytes ◽  
Red Cells ◽  
Sheep Red Cells

scholarly journals CELLS INVOLVED IN THE IMMUNE RESPONSE

1969 ◽  
Vol 129 (4) ◽  
pp. 757-774 ◽  
Author(s):  
Nabih I. Abdou ◽  
Maxwell Richter
Keyword(s):  
Bone Marrow ◽  
Immune Response ◽  
Red Blood Cells ◽  
Blood Cells ◽  
Bone Marrow Cells ◽  
Allogeneic Bone Marrow ◽  
Red Cells ◽  
Allogeneic Bone ◽  
Sheep Red Blood Cells ◽  
Sheep Red Cells

Irradiated rabbits given allogeneic bone marrow cells from normal adult donors responded to an injection of sheep red blood cells by forming circulating antibodies. Their spleen cells were also capable of forming many plaques using the hemolysis in gel technique, and were also capable of undergoing blastogenesis and mitosis and of incorporating tritiated thymidine upon exposure to the specific antigen in vitro. However, irradiated rabbits injected with allogeneic bone marrow obtained from rabbits injected with sheep red blood cells 24 hr prior to sacrifice (primed donors) were incapable of mounting an immune response after stimulation with sheep red cells. This loss of reactivity by the bone marrow from primed donors is specific for the antigen injected, since the immune response of the irradiated recipients to a non-cross-reacting antigen, the horse red blood cell, is unimpaired. Treatment of the bone marrow donors with high-titered specific antiserum to sheep red cells for 24 hr prior to sacrifice did not result in any diminished ability of their bone marrow cells to transfer antibody-forming capacity to sheep red blood cells. The significance of these results, with respect to the origin of the antigen-reactive and antibody-forming cells in the rabbit, is discussed.

scholarly journals STUDIES ON THE ANTIGENIC PROPERTIES OF COMPLEMENT

1960 ◽  
Vol 111 (1) ◽  
pp. 93-106 ◽  
Author(s):  
Paul G. Klein ◽  
Peter M. Burkholder
Keyword(s):  
Guinea Pig ◽  
Red Cells ◽  
Serum Globulin ◽  
Sheep Red Cells ◽  
Antigenic Properties ◽  
Pig Serum

Evidence is presented to show that guinea pig complement fixed on sensitized sheep red cells acts as a specific agglutinogen. Agglutinating antibodies that react with cell-fixed complement can be produced by immunizing rabbits with a complex of stromata-amboceptor-complement or with guinea pig serum globulin. These agglutinins can be removed by precipitation with guinea pig serum. They are, therefore, distinct from immunoconglutinins.

A NON-SPECIFIC DEPRESSANT EFFECT ON THE IMMUNOLOGICAL RESPONSE

1962 ◽  
Vol 40 (10) ◽  
pp. 1353-1358
Author(s):  
Robert Bruce ◽  
Bernhard Cinader ◽  
John Percy
Keyword(s):  
Amino Acids ◽  
Antibody Response ◽  
Immunological Response ◽  
Red Cells ◽  
Depressant Effect ◽  
Intraperitoneal Route ◽  
Sheep Red Cells

The antibody response of mice to intraperitoneally injected sheep red cells was found to be markedly depressed by the injection of isogenic mouse red cells (1,2) given 1–3 days before the antigen. If the isogenic cells were administered at the same time or 1 day after the antigen, the antibody response was not affected. Other non-antigenic substances such as a 5% solution of amino acids exercised a similar depressant effect. The depression of the antibody response did not occur when the isogenic red cells were given intraperitoneally and the antigen by another route. When the effect of specific cells or agents on the antibody response is to be examined it is frequently necessary to make two injections. Only one of the two should be made by the intraperitoneal route.

Swelling, NEM, and A23187 activate Cl(-)-dependent K+ transport in high-K+ sheep red cells

1987 ◽  
Vol 252 (2) ◽  
pp. C197-C204 ◽  
Author(s):  
H. Fujise ◽  
P. K. Lauf
Keyword(s):  
Metal Ion ◽  
Red Cells ◽  
Common Mechanism ◽  
Ionophore A23187 ◽  
Sheep Red Cells ◽  
High K ◽  
Sh Group ◽  
Low K ◽  
K Transport ◽  
Significant Activation

In low K+ (LK) sheep red cells a significant fraction of the total ouabain-resistant (OR) K+ flux is inhibited when Cl- is replaced by other anions of the Hofmeister series except Br- (Cl(-)-dependent K+ flux). In contrast, high K+ (HK) sheep red cells in isosmotic media did not possess any significant OR Cl(-)-dependent K+ flux when Cl- was replaced by NO3- or I-. However, exposure to hyposmotic solutions, treatment with the sulfhydryl (SH) group reagent N-ethylmaleimide (NEM) or with the bivalent metal ion (Me2+) ionophore A23187 in absence of external Me2+ caused a significant activation of Cl(-)-dependent K+ transport as measured with Rb+ as K+ congener. There was no Cl(-)-dependent Rb+ flux in A23187-treated cells when Mn2+, Mg2+, and Ca2+ were present at 1 mM concentrations, suggesting that cellular accumulation of these Me2+ is inhibitory. Similar to LK red cells, HK red cells failed to respond to A23187 when pretreated with NEM supporting the hypothesis proposed recently (Lauf, P. K. J. Membr. Biol. 88: 1-13, 1985) of a common mechanism of Cl(-)-dependent K+ transport activation. The magnitudes of the Cl(-)-dependent Rb+ fluxes in HK cells were much smaller than those elicited by identical treatments in LK red cells, and the effect of all interventions was not due to the presence of reticulocytes known to possess Cl(-)-dependent K+ transport.(ABSTRACT TRUNCATED AT 250 WORDS)

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