scholarly journals The effects of P O2 upon transepithelial ion transport in fetal rat distal lung epithelial cells

2000 ◽  
Vol 524 (2) ◽  
pp. 539-547 ◽  
Author(s):  
S. J. Ramminger ◽  
D. L. Baines ◽  
R. E. Olver ◽  
S. M. Wilson
Keyword(s):  
Epithelial Cells ◽  
Ion Transport ◽  
Lung Epithelial Cells ◽  
Fetal Rat ◽  
Lung Epithelial ◽  
Distal Lung

H2O2 mediates O2 toxicity in cultured fetal rat distal lung epithelial cells

1999 ◽  
Vol 26 (11-12) ◽  
pp. 1357-1368 ◽  
Author(s):  
Xiaoping Luo ◽  
Neil A Christie ◽  
Michael A McLaughlin ◽  
Rose Belcastro ◽  
Larisa Sedlackova ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Lung Epithelial Cells ◽  
Fetal Rat ◽  
Lung Epithelial ◽  
Distal Lung

scholarly journals P2Y2 receptor-mediated inhibition of ion transport in distal lung epithelial cells

1999 ◽  
Vol 128 (2) ◽  
pp. 293-300 ◽  
Author(s):  
S J Ramminger ◽  
A Collett ◽  
D L Baines ◽  
H Murphie ◽  
H L McAlroy ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Ion Transport ◽  
Lung Epithelial Cells ◽  
P2y2 Receptor ◽  
Lung Epithelial ◽  
Distal Lung

Fetal lung cell-derived matrix alters distal lung epithelial ion transport

1995 ◽  
Vol 268 (5) ◽  
pp. L762-L771 ◽  
Author(s):  
O. M. Pitkanen ◽  
A. K. Tanswell ◽  
H. M. O'Brodovich
Keyword(s):  
Epithelial Cells ◽  
Ion Transport ◽  
Short Circuit ◽  
Alveolar Epithelial Cells ◽  
Lung Epithelial Cells ◽  
Bathing Solution ◽  
Type I ◽  
Ussing Chambers ◽  
Lung Epithelial ◽  
Distal Lung

Extracellular matrix (ECM) synthesized by the fetal mesenchymal cells provides a supporting structure for the growing airways and is important for airway branching and in the differentiation of the primitive epithelium. We studied whether ECM, in addition to its structural role in lung interstitium, influences the ion transport of rat fetal distal lung epithelial cells (FDLE). FDLE monolayers were cultured on two different fetal mixed lung cell (MLC)-derived matrix preparations and studied in Ussing chambers. FDLE on MLC matrix had an increased resting equivalent short-circuit current (Ieq). Amiloride (10(-4) M apically) decreased the Ieq significantly in all the FDLE monolayers. The residual Ieq was significantly larger in FDLE grown on MLC matrix (increased by 150 and 80% under baseline and beta 2-agonist-stimulated conditions, respectively) than on control filters and filters coated with type I collagen, and type IV collagen, laminin, or fibronectin. The matrix produced by MLC isolated at an earlier gestational stage decreased the FDLE's sensitivity to amiloride. The increased amiloride-insensitive Ieq was only modestly affected by the Na+/K+/Cl- cotransport inhibitor bumetanide (10(-4) M basally) but was abolished when the [Cl-] of the bathing solution was reduced to 10 mM. These observations demonstrated that MLC elaborated ECM is able to change the nature of the ion transport of FDLE. ECM may be an important factor governing the ion transporting phenotype of fetal type II alveolar epithelial cells.

Keratinocyte Growth Factor Stimulates CLC-2 Expression in Primary Fetal Rat Distal Lung Epithelial Cells

1999 ◽  
Vol 20 (4) ◽  
pp. 842-847 ◽  
Author(s):  
Carol J. Blaisdell ◽  
Jason P. Pellettieri ◽  
Ceila E. Loughlin ◽  
Shijian Chu ◽  
Pamela L. Zeitlin
Keyword(s):  
Growth Factor ◽  
Epithelial Cells ◽  
Keratinocyte Growth Factor ◽  
Lung Epithelial Cells ◽  
Fetal Rat ◽  
Lung Epithelial ◽  
Distal Lung

Growth of distal fetal rat lung epithelial cells in a defined serum-free medium

1991 ◽  
Vol 27 (8) ◽  
pp. 625-632 ◽  
Author(s):  
D. Jassal ◽  
R. N. N. Han ◽  
I. Caniggia ◽  
M. Post ◽  
A. K. Tanswell
Keyword(s):  
Epithelial Cells ◽  
Lung Epithelial Cells ◽  
Rat Lung ◽  
Serum Free Medium ◽  
Free Medium ◽  
Serum Free ◽  
Fetal Rat ◽  
Lung Epithelial ◽  
Fetal Rat Lung

Inhibition of amiloride-sensitive sodium-channel activity in distal lung epithelial cells by nitric oxide

1998 ◽  
Vol 274 (3) ◽  
pp. L378-L387 ◽  
Author(s):  
Jin Wen Ding ◽  
John Dickie ◽  
Hugh O’Brodovich ◽  
Yutaka Shintani ◽  
Bijan Rafii ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Epithelial Cells ◽  
Cell Function ◽  
Short Circuit ◽  
Short Circuit Current ◽  
Lung Epithelial Cells ◽  
Flufenamic Acid ◽  
Activated Macrophages ◽  
Lung Epithelial ◽  
Distal Lung

Distal lung epithelial cells (DLECs) play an active role in fluid clearance from the alveolus by virtue of their ability to actively transport Na+ from the alveolus to the interstitial space. The present study evaluated the ability of activated macrophages to modulate the bioelectric properties of DLECs. Low numbers of lipopolysaccharide (LPS)-treated macrophages were able to significantly reduce amiloride-sensitive short-circuit current ( I sc) without affecting total I sc or monolayer resistance. This was associated with a rise in the flufenamic acid-sensitive component of the I sc. The effect was reversed by the addition of N-monomethyl-l-arginine to the medium, implying a role for nitric oxide. We hypothesized that macrophages exerted their effect by expressing inducible nitric oxide synthase (iNOS) in DLECs. The products of LPS-treated macrophages increased the levels of iNOS protein and mRNA transcripts in DLECs as well as causing a rise in iNOS activity. Immunofluorescence microscopy of LPS-stimulated macrophage-DLEC cocultures with anti-nitrotyrosine antibodies provided evidence for the generation of peroxynitrite in macrophages but not in DLECs. These data indicate that activated macrophages in the lung may contribute to impaired resolution of acute respiratory distress syndrome and suggest a novel mechanism whereby nitric oxide might alter cell function by altering its ion-transporting phenotype.

scholarly journals Mechanotransduction via TRPV4 regulates inflammation and differentiation in fetal mouse distal lung epithelial cells

2015 ◽  
Vol 16 (1) ◽  
Author(s):  
Pritha S. Nayak ◽  
Yulian Wang ◽  
Tanbir Najrana ◽  
Lauren M. Priolo ◽  
Mayra Rios ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Lung Epithelial Cells ◽  
Fetal Mouse ◽  
Lung Epithelial ◽  
Distal Lung
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