ALKALINE PHOSPHOMONOESTERASE ACTIVITY OF GOAT BRAIN CORTEX RIBOSOMES

1. Ribosomes isolated from the cortex tissue of goat brain contain very small amounts of spermidine and spermine. Ribosomes isolated from spermidine-treated slices have a higher spermidine content. 2. The polyamines partially prevent the temperature-dependent breakdown of ribosomes into acid-soluble nucleotides. 3. The ‘melting’ temperature of ribosomes rises slightly when the ribosomes are heated slowly in the presence of polyamines. 4. The pH-dependent breakdown of ribosomes into protein, RNA and acid-soluble nucleotide is markedly decreased by polyamines present in media in which ribosomes are suspended. 5. The breakdown of ribosomes in the presence of high concentrations of salts and EDTA is partially checked by the concurrent presence of polyamines. 6. Spermidine and spermine make ribosomes less susceptible to enzymic digestion by crystalline trypsin and ribonuclease.

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ALKALINE PHOSPHODIESTERASE ACTIVITY OF GOAT BRAIN CORTEX RIBOSOMES

Journal of Neurochemistry ◽

10.1111/j.1471-4159.1969.tb08976.x ◽

1969 ◽

Vol 16 (6) ◽

pp. 875-887 ◽

Cited By ~ 2

Author(s):

R. K. Datta ◽

J. J. Ghosh ◽

K. C. Bhattacharyya

Keyword(s):

Brain Cortex ◽

Phosphodiesterase Activity ◽

Goat Brain

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Mescaline-induced changes of brain-cortex ribosomes. Effect of mescaline on the stability of brain-cortex ribosomes

Biochemical Journal ◽

10.1042/bj1170961 ◽

1970 ◽

Vol 117 (5) ◽

pp. 961-968 ◽

Cited By ~ 9

Author(s):

R. K. Datta ◽

J. J. Ghosh

Keyword(s):

Enzyme Activities ◽

Brain Cortex ◽

Rapid Degradation ◽

Brain Cortex Slices ◽

Cortex Slices ◽

The Stability ◽

Induced Changes ◽

Goat Brain ◽

Absorption Characteristics ◽

Positive Materials

1. During the action of mescaline sulphate on goat brain-cortex slices the ribosomal particles become susceptible to breakdown, releasing protein, RNA, acidsoluble nucleotides and ninhydrin-positive materials, resulting in loss of ribosomal enzyme activities. 2. Ribosomes of the mescaline-treated cortex slices undergo rapid degradation in the presence of trypsin and ribonuclease. 3. Mescaline does not alter the chemical and nucleotide compositions or the u.v.-absorption characteristics of ribosomal particles, however.

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Alkaline phosphomonoesterase activity in insects—I. Activity in various enzyme preparations from the blowflies, Phormia regina and Sarcophaga bullata

Journal of Insect Physiology ◽

10.1016/0022-1910(63)90114-8 ◽

1963 ◽

Vol 9 (3) ◽

pp. 383-389 ◽

Cited By ~ 1

Author(s):

Ernest Hodgson

Keyword(s):

Phormia Regina ◽

Enzyme Preparations ◽

Sarcophaga Bullata ◽

Alkaline Phosphomonoesterase ◽

Phosphomonoesterase Activity

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Mescaline-induced changes of brain-cortex ribosomes. Effect of mescaline on the hydrogen-bonded structure of ribonucleic acid of brain-cortex ribosomes

Biochemical Journal ◽

10.1042/bj1170969 ◽

1970 ◽

Vol 117 (5) ◽

pp. 969-980 ◽

Cited By ~ 5

Author(s):

R. K. Datta ◽

J. J. Ghosh

Keyword(s):

Ribosomal Rna ◽

Ribonucleic Acid ◽

Brain Cortex ◽

Total Rna ◽

Hyperchromic Effect ◽

Brain Cortex Slices ◽

Cortex Slices ◽

Induced Changes ◽

Goat Brain ◽

Hydrogen Bonded

1. The action of mescaline sulphate on the hydrogen-bonded structure of the RNA constituent of ribosomes of goat brain-cortex slices was studied by using the hyperchromic effect of heating and formaldehyde reaction. 2. The ribosomal total RNA species of the mescaline-treated brain-cortex slices have a smaller proportion of hydrogen-bonded structure than the ribosomal RNA species of the untreated brain-cortex slices. 3. Mescaline also appears to have affected this lowering of hydrogen-bonded structure of the ribosomal 28S RNA of brain-cortex tissue.

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Mescaline-induced changes of brain-cortex ribosomes. Role of spermidine in counteracting the destabilizing effect of mescaline on brain-cortex ribosomes

Biochemical Journal ◽

10.1042/bj1250213 ◽

1971 ◽

Vol 125 (1) ◽

pp. 213-219 ◽

Cited By ~ 6

Author(s):

R. K. Datta ◽

W. Antopol ◽

J. J. Ghosh

Keyword(s):

Brain Cortex ◽

Heat Denaturation ◽

Brain Cortex Slices ◽

Cortex Slices ◽

Induced Changes ◽

Goat Brain

1. The effect of spermidine on the mescaline-induced changes of brain-cortex ribosomes was studied by adding spermidine during the treatment of goat brain-cortex slices with mescaline. 2. Mescaline treatment of brain-cortex slices removed a portion of the endogenous spermidine from ribosomes and this removal was significantly prevented when spermidine was present during mescaline treatment. 3. Spermidine present during mescaline treatment of brain-cortex slices counteracted, to some extent, the destabilizing effect of mescaline on ribosomes with respect to heat denaturation. 4. Mescaline treatment of brain-cortex slices made ribosomes more susceptible to breakdown, releasing protein and RNA, and resulting in loss of ribosomal enzymic activities. However, spermidine present during mescaline treatment counteracted moderately the mescaline-induced ribosomal susceptibility to breakdown and ribosomal loss of enzymic activities. 5. Ribosomes of mescaline-treated cortex slices were rapidly degraded by ribonuclease and trypsin. However, if spermidine was present during mescaline treatment of brain-cortex slices the rates of degradation diminished.

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