THE RELATIONSHIP BETWEEN AMINO ACID INCORPORATION INTO PROTEIN IN ISOLATED NEOCORTEX SLICES AND THE TISSUE CONTENT OF FREE AMINO ACID

1972 ◽  
Vol 19 (3) ◽  
pp. 779-790 ◽  
Author(s):  
D. A. Jones
1975 ◽  
Vol 146 (2) ◽  
pp. 395-400 ◽  
Author(s):  
M Nwagwu

The efficiencies of two chromatographic species of [3-H]seryl-tRNA, namely peaks I and II, in cell-free amino acid incorporation were investigated. The maximum yield of polypeptide seems to be the same for the reaction mixtures containing either peak I or peak II, suggesting that the efficiency of both peaks in total protein synthesis is the same. The efficiency of transfer of serine into myosin heavy subunit (myosin H) by peaks I and II was also investigated. Peak II of [3-H]seryl-tRNA transfers three times as much serine into myosin H as peak I.


Biochemistry ◽  
1971 ◽  
Vol 10 (13) ◽  
pp. 2581-2587 ◽  
Author(s):  
Fritz Rottman ◽  
Brian E. Dunlap ◽  
Karen H. Friderici

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