Mechanisms of Toxicity and Cellular Resistance to 1-Methyl-4-Phenyl-1,2,3,6-Tetrahydropyridine and 1-Methyl-4-Phenylpyridinium in Adrenomedullary Chromaffin Cell Cultures

1990 ◽  
Vol 55 (1) ◽  
pp. 311-320 ◽  
Author(s):  
John F. Reinhard ◽  
Stephen W. Carmichael ◽  
Alejandro J. Daniels
Keyword(s):  
Cell Cultures ◽  
Chromaffin Cell ◽  
Cellular Resistance ◽  
Mechanisms Of Toxicity

2′,3′-Cyclic nucleotide 3′-phosphodiesterase is associated with mitochondria in diverse adrenal cell types

1997 ◽  
Vol 110 (23) ◽  
pp. 2979-2985
Author(s):  
B. McFerran ◽  
R. Burgoyne
Keyword(s):  
Cell Cultures ◽  
Cyclic Nucleotide ◽  
Chromaffin Cells ◽  
Chromaffin Cell ◽  
Pcr Amplification ◽  
Cell Types ◽  
Adrenal Cell ◽  
Immunofluorescence Studies ◽  
Mitochondrial Staining ◽  
Intracellular Localisation

In this study, we have examined the expression and intracellular localisation of the myelin protein 2′,3′-cyclic nucleotide 3′-phosphodiesterase (CNP) in bovine adrenal medullary chromaffin cell cultures. By immunoblotting, using two distinct anti-CNP monoclonal antibodies, CNP was detected in medullary cell cultures and expression of CNP was confirmed by reverse transcription and PCR amplification. CNP did not leak from digitonin-permeabilised chromaffin cells, suggesting that there is no cytosolic pool of this protein. Immunofluorescence studies with both antibodies showed that all cells in the medullary chromaffin cell culture were stained with a punctate appearance consistent with an intracellular localisation for CNP. More specifically it was demonstrated that CNP is co-localised with mitochondria. Various cell types in chromaffin cell cultures were stained with a mitochondrial pattern and CNP staining was co-localised with mitochondrial staining. These results show that CNP is a widely expressed protein that is associated with mitochondria and provides new clues as to its cellular function outside of myelin structures.

Production of “ectopic” vasoactive intestinal peptide-like immunoreactivity in normal human chromaffin cell cultures

Life Sciences ◽  
1985 ◽  
Vol 37 (20) ◽  
pp. 1881-1886 ◽  
Author(s):  
Arthur S. Tischler ◽  
Ying C. Lee ◽  
Robert L. Perlman ◽  
Donna Costopoulos ◽  
Stephen R. Bloom
Keyword(s):  
Vasoactive Intestinal Peptide ◽  
Cell Cultures ◽  
Chromaffin Cell ◽  
Normal Human

Potentiation of mitogenesis in adult rat chromaffin cell cultures by immunosuppressive agent FK506

2004 ◽  
Vol 356 (1) ◽  
pp. 5-8 ◽  
Author(s):  
J.F. Powers ◽  
J.M. Brachold ◽  
K. Schelling ◽  
A.S. Tischler
Keyword(s):  
Cell Cultures ◽  
Chromaffin Cell ◽  
Immunosuppressive Agent ◽  
Adult Rat
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