A Versatile AIE Fluorogen with Selective Reactivity to Primary Amines for Monitoring Amination, Protein Labeling and Mitochondrial Staining

Specific bioconjugation for native primary amines is highly valuable for both chemistry and biomedical research. Despite all the efforts, scientists lack a proper strategy to achieve high selectivity for primary amines, not to mention the requirement of fast response for real applications. Herein, in this work, we report a chromone-based aggregation-induced emission (AIE) fluorogen called CMVMN as a self-reporting bioconjugation reagent for selective primary amine identification, and its applications for monitoring bioprocesses of amination and protein labeling. CMVMN is AIE-active and is capable of solid-state sensing. Thus, its electrospun films are manufactured for visualization of amine diffusion and leakage process. CMVMN also shows good biocompatibility and potential mitochondria-staining ability, which provides new insight for organelle-staining probe design. Combined with its facile synthesis and good reversibility, CMVMN not only shows wide potential applications in biology, but also offers new possibilities for molecular engineering.

Rh(III)-catalysed synthesis of cinnolinium and fluoranthenium salts by using C-H activation/annulation reactions: Organelle specific mitochondrial staining application

Construction of new class indazolo[2,1-a]cinnolin-7-ium, and diazabenzofluoranthenium salts were developed by using Rh(III)-catalyzed C-H activation/annulation reactions with 2-phenyl-2H-indazole, and internal alkynes, which resulted in a structurally important polycyclic heteroaromatic compounds...

The application of amide units in the construction of neutral functional dyes for mitochondrial staining

To develop a new class of neutral fluorescent dyes with mitochondrial staining capacity, a series of functional dyes were obtained from Nile red (2a–e) and coumarin (3a–e) with different amide compounds via Suzuki coupling reactions.

When the strategies for cellular selectivity fail. Challenges and surprises in the design and application of fluorescent benzothiadiazole derivatives for mitochondrial staining

Design, synthesis, molecular architecture and the unexpected behavior of fluorescent benzothiadiazole for selective mitochondrial and plasma membrane staining are investigated.

‘Aggregation induced emission’ active iridium(iii) complexes with applications in mitochondrial staining

Synthesized two new bis-cyclometalated iridium(iii) complexes exhibiting strong AIE, studied their luminescence by spectroscopy and quantum chemical calculations and applied as a non-toxic bio-imaging probe for mitochondrial staining.