Development of a modified selective medium to enhance the recovery rate of Brachyspira hyodysenteriae and other porcine intestinal spirochaetes from faeces

Burkholderia cepacia colonizes cystic fibrosis (CF) patients. We evaluated the impact of the use of a selective medium in the rate of B. cepacia recovery from respiratory samples of CF patients. During a 6-month period, respiratory samples were collected from 106 CF patients and cultivated on selective media including a B. cepacia selective medium. Confirmation of the identity of B. cepacia isolates was carried out by species specific PCR and determination of genomovar status performed by a sequential PCR approach. Results of B. cepacia isolation during this period were compared to the preceding two years, when the sample processing was identical except for the lack of the B. cepacia selective medium. B. cepacia was isolated in 11/257 (4.2%) of the samples using the selective medium, in contrast with the preceding two years, when it was isolated in 6/1029 samples (0.58%), p < 0.0001. Identity of all 11 isolates was confirmed by PCR and genomovar determination was accomplished in all but one isolate. These results suggest that the use of a selective medium increases recovery rate of B. cepacia from respiratory samples.

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Comparison of BCYE and BMPA media on recovery rate of Legionella pneumophila

Health Science Journal of Indonesia ◽

10.22435/hsji.v11i1.3127 ◽

2020 ◽

Vol 11 (1) ◽

pp. 32-37

Author(s):

Lucky Moehario ◽

Enty Tjoa ◽

Mutiara J. Taslim ◽

Yohanna Angelina

Keyword(s):

Yeast Extract ◽

Legionella Pneumophila ◽

Recovery Rate ◽

Culture Method ◽

Colony Growth ◽

Selective Medium ◽

Plate Count ◽

Surveillance Program ◽

Specific Medium ◽

Routine Surveillance

Latar belakang: Legionella pneumophila (L. pneumophila) telah banyak diketahui sebagai penyebab legionellosis; habitat nya di berbagai sumber air, lingkungan lembab dan hangat. Metode kultur menggunakan media spesifik masih menjadi baku emas untuk identifikasi L. pneumophila. Penelitian ini bertujuan membandingkan recovery rate L. pneumophila ATCC®33823 pada medium spesifik BCYE, dan medium selektif BMPA. Metode: Dilakukan dilusi serial pada suspensi 0,5 McFarland L. pneumophila ATCC®33823; 100 ul dari tiap tingkat dilusi diinokulasi pada medium Buffered Charcoal Yeast Extract (BCYE) dan medium BMPA (medium BCYE yang ditambahkan suplemen BMPA-α), secara duplo. Konsentrasi suspensi dihitung menggunakan metode Angka Lempeng Total (ALT) dari Standar Nasional Indonesia No. 01-2332.3-2006. Persentase recovery rate dihitung dan dianalisis secara statistik menggunakan SPSS versi 23,0. Hasil: Jumlah koloni L. pneumophila yang tumbuh pada medium BMPA jauh lebih tinggi daripada yang tumbuh di medium BCYE; konsentrasi tertinggi yang diperoleh adalah pada medium BMPA sebesar 1,45 x 107 CFU/ml. Persentase recovery rate pada medium BMPA adalah 96,67%, dan 60,67% pada medium BCYE. Kesimpulan: Recovery rate medium BMPA untuk pertumbuhan koloni L. pneumophila ATCC®33823 jauh lebih tinggi daripada media BCYE, karena itu medium BMPA dapat direkomendasikan untuk kultivasi L. pneumophila, khususnya pada program surveilans berbagai sumber air dengan biaya lebih terjangkau. Kata kunci: Legionella pneumophila, medium spesifik, BCYE , BMPA, recovery rate Abstract Background: Legionella pneumophila (L. pneumophila) has been known as the etiology of legionellosis; they live in aquatic environment, warm and moist. Culture method using specific medium remains as the gold standard in the identification of L. pneumophila. This study aimed to compare the recovery rate of L. pneumophila ATCC® 33823 on the specific medium BCYE for the cultivation of Legionella, and BMPA, the selective medium. Methods: Suspension of L. pneumophila ATCC® 33823 of 0.5 McFarland was diluted to 10 fold serial dilution; 100 ul of each dilution was inoculated on Buffered Charcoal Yeast Extract (BCYE) medium, and BMPA (BCYE supplemented with BMPA-α) in duplicate manner. The concentration was calculated using Total Plate Count standard as of Indonesian Nasional Standard number 01-2332.3-2006. The percentage of recovery rate was calculated, and the statistical analysis was performed using SPSS version 23.0. Results: Numbers of colonies of L. pneumophila grew on BMPA was much higher than on BCYE medium; the highest concentration was yielded on BMPA medium i.e. 1.45x107 CFU/ml. The recovery rates were 96.67% and 60.67% on BMPA medium and BCYE subsequently. Conclusion: The recovery rate of the BMPA medium on the colony growth of L. pneumophila ATCC®33823 was markedly higher than the BCYE, therefore BMPA medium can be suggested to be used in the cultivation of L. pneumophila especially in the routine surveillance program for water sources with less cost. Keywords: Legionella pneumophila, specific medium, BCYE , BMPA, recovery rate

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Comparison of Preston Agar and a Blood-Free Selective Medium for Detection of Campylobacter jejuni in Food

Journal of AOAC INTERNATIONAL ◽

10.1093/jaoac/74.4.651 ◽

1991 ◽

Vol 74 (4) ◽

pp. 651-654

Author(s):

Mats Peterz

Keyword(s):

Campylobacter Jejuni ◽

Recovery Rate ◽

Collaborative Study ◽

Selective Medium ◽

Chicken Liver ◽

Agar Media ◽

Better Than

Abstract The present collaborative study compares recovery of Campylobacter Jejuni from food in 2 agar media. Six laboratories analyzed 8 samples each of chicken liver Inoculated with Campylobacter Jejuni. Samples were enriched in Preston broth and isolation was carried out on Preston agar (PA) and Campylobacter blood-free selective medium (CBFS), a charcoal- based medium with cefoperazone and amphoteracin as antibiotic supplements. There was no difference In the recovery rate between the 2 agar media; however, the specificity of CBFS was better than that of PA. There was a slightly better growth of Campylobacters, and competing organisms were more inhibited on CBFS than on PA.

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Selective media for detection and quantification of Brazilian Ralstonia solanacearum isolates in soil

Horticultura Brasileira ◽

10.1590/s0102-053620170107 ◽

2017 ◽

Vol 35 (1) ◽

pp. 41-47 ◽

Cited By ~ 1

Author(s):

Nadson C Pontes ◽

Miriam F Fujinawa ◽

José R Oliveira

Keyword(s):

South Africa ◽

Bacterial Growth ◽

Ralstonia Solanacearum ◽

Recovery Rate ◽

Antimicrobial Agents ◽

Selective Medium ◽

Selective Media ◽

Detection And Quantification

ABSTRACT The efficacy of five selective media described in the literature for the detection of Ralstonia solanacearum in soil was evaluated using isolates from different regions of Brazil. The selective media were evaluated regarding their sensitivity, target-pathogen repression index, capacity for suppressing contaminating microorganisms and recovery rate of the pathogen in soil. No bacterial growth was observed at the concentrations tested for FSM and SM-1 media. Some isolates grew on the PCCG medium with the addition of antimicrobial agents, but only at concentrations of 106 cfu/mL. For the SM and SMSA-E media, adequate sensitivity for the detection of R. solanacearum in soil was observed, enabling detection at a concentration of approximately 103 cfu/gram of soil. SMSA-E (Selective Medium South Africa - Elphinstone) was found to be the most effective in suppressing the growth of contaminating microorganisms, resulting in the lowest rate of repression of R. solanacearum and the highest recovery rate of the bacterium in the soil.

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