scholarly journals G-protein involvement in muscarinic receptor-stimulation of inositol phosphates in longitudinal smooth muscle from the small intestine of the guinea-pig

1995 ◽  
Vol 114 (1) ◽  
pp. 119-126 ◽  
Author(s):  
S.A. Prestwich ◽  
T.B. Bolton
Keyword(s):  
Smooth Muscle ◽  
Small Intestine ◽  
Guinea Pig ◽  
G Protein ◽  
Muscarinic Receptor ◽  
Inositol Phosphates ◽  
Receptor Stimulation ◽  
Longitudinal Smooth Muscle ◽  
Stimulation Of

scholarly journals Relationship between stimulated phosphatidic acid production and inositol lipid hydrolysis in intestinal longitudinal smooth muscle from guinea pig

1987 ◽  
Vol 244 (3) ◽  
pp. 763-768 ◽  
Author(s):  
R S E Mallows ◽  
T B Bolton
Keyword(s):  
Smooth Muscle ◽  
Small Intestine ◽  
Substance P ◽  
Guinea Pig ◽  
Phosphatidic Acid ◽  
Inositol Phosphates ◽  
Muscle Layer ◽  
Phospholipid Hydrolysis ◽  
Longitudinal Smooth Muscle ◽  
Inositol Phospholipid

Accumulation of [32P]phosphatidic acid (PA) and total [3H]inositol phosphates (IPs) was measured in the longitudinal smooth-muscle layer from guinea-pig small intestine. Stimulation with carbachol, histamine and substance P produced increases in accumulation of both [3H]IPs and [32P]PA over the same concentration range. The increase in [32P]PA accumulation in response to carbachol (1 microM-0.1 mM) was inhibited in the presence of atropine (0.5 microM). Buffering the external free [Ca2+] to 10 nM did not prevent the carbachol-stimulated increase in [32P]PA accumulation. Carbachol and Ca2+ appear to act synergistically to increase accumulation of [32P]PA. In contrast, although incubation with noradrenaline also increased accumulation of [3H]IPs, no increase in accumulation of [32P]PA could be detected. These results suggest that an increase in formation of IPs is not necessarily accompanied by an increase in PA formation, and imply the existence of receptor-modulated pathways regulating PA concentrations other than by phospholipase-C-catalysed inositol phospholipid hydrolysis.

scholarly journals Muscarinic-receptor stimulation enhances polyphosphoinositide breakdown in guinea-pig ileum smooth muscle

1984 ◽  
Vol 223 (2) ◽  
pp. 527-531 ◽  
Author(s):  
M C Sekar ◽  
B D Roufogalis
Keyword(s):  
Guinea Pig ◽  
Muscarinic Receptor ◽  
Longitudinal Muscle ◽  
Inositol Phosphates ◽  
Inositol Phosphate ◽  
Exchange Chromatography ◽  
Guinea Pig Ileum ◽  
Receptor Stimulation ◽  
Inositol 1 ◽  
Stimulation Of

Muscarinic-receptor stimulation by 0.1 mM-carbachol in longitudinal muscle of the guinea-pig ileum increases the incorporation of [3H]inositol into inositol-containing phospholipid. This effect was blocked by 16 microM-atropine. After 60 min incubation, carbachol increased the accumulation of total inositol phosphates 20-fold in the presence of 10 mM-Li+. Less than 20% of the total inositol phosphate corresponded to inositol 1-phosphate by ion-exchange chromatography, whereas of the remainder about two-thirds corresponded to inositol bisphosphate and one third to inositol trisphosphate. It is concluded that stimulation of muscarinic receptors in guinea-pig ileum enhances breakdown of polyphosphoinositides, suggesting that this may be a primary event associated with Ca2+ mobilization in the guinea-pig ileum.

The actions of praziquantel and 1-methyladenine in guinea pig ileal longitudinal muscle

1979 ◽  
Vol 57 (12) ◽  
pp. 1460-1462 ◽  
Author(s):  
K. Jim ◽  
D. J. Triggle
Keyword(s):  
Smooth Muscle ◽  
Guinea Pig ◽  
Muscarinic Receptor ◽  
Longitudinal Muscle ◽  
Receptor Stimulation ◽  
Extracellular Concentration ◽  
Mechanical Responses ◽  
Channel Function ◽  
Longitudinal Smooth Muscle

Praziquantel (10−4 M) and 1-methyladenine (5 × 10−4 M) produced mechanical responses in guinea pig ileal longitudinal smooth muscle that were approximately 20% of those produced by muscarinic receptor stimulation. These responses were insensitive to tetrodotoxin and atropine but were dependent upon the extracellular concentration of Ca2+ and were abolished by D 600 at a concentration (10−6 M) which abolished the Ca2+-dependent muscarinic responses. Thus, praziquantel and 1-methyladenine may act as modulators of Ca2+ channel function in guinea pig ileal muscle.

scholarly journals Muscarinic cholinergic stimulation of phosphatidylinositol turnover in the longitudinal smooth muscle of guinea-pig ileum

1976 ◽  
Vol 154 (3) ◽  
pp. 653-657 ◽  
Author(s):  
S S. Jafferji ◽  
R H. Michell
Keyword(s):  
Smooth Muscle ◽  
Guinea Pig ◽  
Phosphatidyl Inositol ◽  
Anticholinergic Drugs ◽  
Muscarinic Agonists ◽  
Guinea Pig Ileum ◽  
High Concentration ◽  
Longitudinal Smooth Muscle ◽  
Muscarinic Cholinergic ◽  
Stimulation Of

1. The metabolism of phosphatidylinositol and phosphatidate was investigated in fragments of longitudinal smooth muscle from guinea-pig ileum incubated with cholinergic and anticholinergic drugs. 2. Incorporation of Pi into these lipids was enhanced by acetylcholine and carbamoylcholine. 3. The receptor responsible for triggering this response was of the muscarinic type, since (a) the response was also produced by the muscarinic agonists acetyl-β-methylcholine, carbamoyl-β-methylcholine and pilocarpine, and (b) the response was prevented by atropine and prophylbenzilylcholine mustard, but not by tubocurarine. 4. Increased phosphatidylinositol labellin was clearly observed within 5 min in tissue treated with a high concentration of carbamoylcholine. 5. Halfmaximal stimulation of phosphatidylinositol labelling occurred at approx. 10 muM-muM-carbamoylcholine. 6. Incubation of muscle fragments with carbamoylcholine provoked a decrease in phosphatidylinositol concentration, as would be expected if phosphatidyl-inositol breakdown is the reaction controlled by agonists. 7. This information all appears consistent with the proposal that phosphatidylinositol breakdown may be a reaction intrinsic to the mechanisms of muscarinic cholinergic receptor systems.

EndothelinB receptor on guinea pig small intestinal smooth muscle cells

1992 ◽  
Vol 262 (2) ◽  
pp. G308-G311 ◽  
Author(s):  
M. Yoshinaga ◽  
Y. Chijiiwa ◽  
T. Misawa ◽  
N. Harada ◽  
H. Nawata
Keyword(s):  
Smooth Muscle ◽  
Small Intestine ◽  
Guinea Pig ◽  
Smooth Muscle Cells ◽  
Muscle Cells ◽  
Binding Characteristics ◽  
Longitudinal Smooth Muscle ◽  
Etb Receptor ◽  
Small Intestinal ◽  
Functional Receptor

We investigated the binding characteristics of the endothelin (ET) receptor and the mechanism by which ET induces contraction of longitudinal smooth muscle cells of the guinea pig small intestine by using vasoactive intestinal contractor (VIC), a mouse variant of ET-2. A functional receptor for VIC was found to exist on longitudinal smooth muscle cells. These cells showed a similar binding of and contractile response to ET-1, ET-2, and ET-3. Inhibitors of both intracellular and extracellular Ca2+ movement attenuated the VIC-induced contraction of longitudinal smooth muscle cells. These results suggest that smooth muscle cells of the guinea pig small intestine express the ETB receptor that primarily mediates the contractile effect on smooth muscle cells. In addition, ET-induced contraction depends on intracellular as well as extracellular Ca2+.

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