scholarly journals Modulation of calcium channel currents by arachidonic acid in single smooth muscle cells from vas deferens of the guinea-pig

1995 ◽  
Vol 116 (2) ◽  
pp. 1887-1893 ◽  
Author(s):  
Norihiro Nagano ◽  
Minora Watanabe
Keyword(s):  
Arachidonic Acid ◽  
Smooth Muscle ◽  
Guinea Pig ◽  
Smooth Muscle Cells ◽  
Calcium Channel ◽  
Vas Deferens ◽  
Muscle Cells ◽  
Channel Currents

Effects of arachidonic acid on A-type potassium currents in smooth muscle cells of the guinea pig

1997 ◽  
Vol 272 (3) ◽  
pp. C860-C869 ◽  
Author(s):  
N. Nagano ◽  
Y. Imaizumi ◽  
M. Watanabe
Keyword(s):  
Fatty Acids ◽  
Arachidonic Acid ◽  
Smooth Muscle ◽  
Guinea Pig ◽  
Smooth Muscle Cells ◽  
Vas Deferens ◽  
Muscle Cells ◽  
Atrial Myocytes ◽  
K Channels ◽  
Type K

Effects of arachidonic acid (AA) and related fatty acids on Ca2+ -independent transient (A-type) K+ current (I(A)) were examined in single myocytes of guinea pig vas deferens, ureter, and proximal colon as well as in rabbit vas deferens. The peak amplitude of I(A) was reduced by external application of AA (half-maximal inhibitory concentration = approximately 1 microM). The blocking effect was not changed significantly by indomethacin, nordihydroguaiaretic acid, guanosine 5'-O-(2-thiodiphosphate), or guanosine 5'-O-(3-thiotriphosphate). Pharmacological studies suggested that the effect of AA was not mediated by activation of protein kinases A or C or tyrosine kinase. AA (20:4) was the most potent of the four types of cis-eicosanoic acids with two to five double bonds (20:2 to 20:5) that were tested. I(A)-like current in cardiac atrial myocytes of the rabbit was not affected significantly by 30 microM AA. These results indicate that AA itself directly blocks A-type K+ channels. A relationship between stereospecific chemical structure of fatty acids and their blockade of A-type K+ channels is suggested. A-type K+ channels in smooth muscle cells can be clearly resolved from those in atrial myocytes by the responses to AA.

scholarly journals Local Ca2+transients and distribution of BK channels and ryanodine receptors in smooth muscle cells of guinea-pig vas deferens and urinary bladder

2001 ◽  
Vol 534 (2) ◽  
pp. 313-326 ◽  
Author(s):  
Yoshiaki Ohi ◽  
Hisao Yamamura ◽  
Norihiro Nagano ◽  
Susumu Ohya ◽  
Katsuhiko Muraki ◽  
...  
Keyword(s):  
Smooth Muscle ◽  
Urinary Bladder ◽  
Guinea Pig ◽  
Smooth Muscle Cells ◽  
Vas Deferens ◽  
Ryanodine Receptors ◽  
Muscle Cells ◽  
Bk Channels
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