Activation and Impairment of Platelet Function In Vitro by Fatty Acid Ethyl Ester, a Nonoxidative Ethanol Metabolite: Effect of Fatty Acid Ethyl Esters on Human Platelets

Fatty acid ethyl ester (FAEE) merupakan salah satu biomarker konsumsi etanol. Penelitian ini bertujuan untuk menganalisis senyawa FAEE dalam sampel rambut secara kualitatif dengan GC-MS. Pada penelitian ini dibuat campuran standar FAEE (Etil Pamlitat dan Etil Oleat) 100 ppm dengan perbandingan 1:1 kemudian dibuat variasi konsentrasi. Metode validasi standar FAEE diukur menggunakan GC-MS. Sampel rambut dipreparasi dengan cara didekontaminasi kemudian diekstraksi dan diinjeksikan ke sistem GC-MS. Hasil validasi diperoleh persamaan regresi linier yaitu, y = 240713,507x-147471,2353 untuk etil palmitat dan y = 58361,45x+242,5 untuk etil oleat dengan nilai koefisien korelasi (r2) berturut-turut sebesar 0,9996 dan 0,9999. Batas deteksi diperoleh sebesar 2,5522 ppm dan 0,0189 ppm, dengan batas kuantitasi sebesar 8,5073 ppm dan 0,0630 ppm. Nilai koefisien variansi diperoleh sebesar 3,59% dan 8,65 x 10-4%, dengan persen perolehan kembali sebesar 100,05% dan 100,08%. Hasil analisis menunjukkan, sampel rambut semua sukarelawan mengandung etil palmitat dan etil oleat pada waktu retensi 22,03 menit dan 23,83 menit. Kata kunci: Fatty acid ethyl esters, Etil Palmitat, Etil Oleat, Alkohol, Etanol, Biomarker, GC-MS

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Synthesis, Purification, and Quantification of Fatty Acid Ethyl Esters After trans-Esterification of Large Batches of Tobacco Seed Oil

Beiträge zur Tabakforschung International/Contributions to Tobacco Research ◽

10.1515/cttr-2015-0008 ◽

2015 ◽

Vol 26 (5) ◽

Cited By ~ 1

Author(s):

Mehdi Ashraf-Khorassani ◽

William Monroe Coleman ◽

Michael Francis Dube ◽

Giorgis Isaac ◽

Larry Thomas Taylor

Keyword(s):

Fatty Acid ◽

Ethyl Ester ◽

Mobile Phase ◽

Large Scale ◽

Seed Oil ◽

Acid Ethyl Ester ◽

Fatty Acid Ethyl Esters ◽

Ethyl Esters ◽

Reaction Products ◽

Tobacco Seed

SummaryThe goal of the study was to quantify fatty acid ethyl esters (FAEE’s) produced from two large batches of tobacco seed oil after trans-esterification by heating in ethanol with sulfuric acid catalyst. Purification of the combined ethyl ester reaction products by removing as much of the color and odor from the final product as possible was achieved via conventional column chromatography with amorphous silica and tandem elution of first hexane and then ethyl alcohol as the mobile phase. Gas chromatography was used to quantify specific FAEE’s in the purified material. Recovery of pure FAEE's in batch #1 was near 87%; while, recovery of FAEE’s in batch #2 was greater than 89% with mass yields greater than 400 g of ethyl esters per esterification trial. The FAEE’s possessed no detectable aroma and only a slight yellow color after this chromatographic treatment. Supercritical fluid chromatography with a mobile phase of methanol/acetonitrile modified carbon dioxide and an octadecyl bonded silica stationary phase were used to characterize the purity of each batch of fatty acid ethyl ester product. No free fatty acids nor glycerolrelated impurities were detected in the purified transesterified product.This is the first report describing the optimized trans-esterification of tobacco seed oil on a relatively large scale coupled with subsequent purification and isolation of the resultant ethyl esters. [Beitr. Tabakforsch. Int. 26 (2015) 205-213]

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