Evaluation of a new generation of plastic culture bottles with an automated microbial detection system for nine common contaminating organisms found in PLT components

Transfusion ◽  
2004 ◽  
Vol 44 (3) ◽  
pp. 359-363 ◽  
Author(s):  
M.E. Brecher ◽  
S.N. Hay ◽  
S.J. Rothenberg
Keyword(s):  
Detection System ◽  
Microbial Detection ◽  
New Generation

scholarly journals Detection of Microbial Nitroreductase Activity by Monitoring Exogenous Volatile Organic Compound Production Using HS-SPME-GC-MS

Separations ◽  
2020 ◽  
Vol 7 (4) ◽  
pp. 64
Author(s):  
Ryan Thompson ◽  
John D. Perry ◽  
Stephen P. Stanforth ◽  
John R. Dean
Keyword(s):  
Organic Compound ◽  
Volatile Organic Compound ◽  
Pathogenic Bacteria ◽  
Solid Phase ◽  
Detection System ◽  
Gas Chromatography Mass Spectrometry ◽  
Microbial Detection ◽  
Enzyme Substrates ◽  
Volatile Organic ◽  
Nitroreductase Activity

Development of a rapid approach for universal microbial detection is required in the healthcare, food and environmental sectors to aid with medical intervention, food safety and environmental protection. This research investigates the use of enzymatic hydrolysis of a substrate by a microorganism to generate a volatile organic compound (VOC). One such enzyme activity that can be used in this context is nitroreductase as such activity is prevalent across a range of microorganisms. A study was developed to evaluate a panel of 51 microorganisms of clinical interest for their nitroreductase activity. Two enzyme substrates, nitrobenzene and 1-fluoro-2-nitrobenzene, were evaluated for this purpose with evolution, after incubation, of the VOCs aniline and 2-fluoroaniline, respectively. Detection of the VOCs was done using headspace-solid phase microextraction-gas chromatography-mass spectrometry (HS-SPME-GC-MS) with obtained limits of quantitation (LOQ) of 0.17 and 0.03 µg/mL for aniline and 2-fluoroaniline, respectively. The results indicated that both enzyme substrates were reduced by the same 84.3% of microorganisms producing the corresponding volatile anilines which were detected using HS-SPME-GC-MS. It was found that nitroreductase activity could be detected after 6–8 h of incubation for the selected pathogenic bacteria investigated. This approach shows promise as a rapid universal microbial detection system.

Cybernetic microbial detection system using transfer learning

2018 ◽  
Vol 79 (7-8) ◽  
pp. 5225-5242 ◽  
Author(s):  
Dinesh Jackson Samuel R ◽  
Rajesh Kanna B
Keyword(s):  
Transfer Learning ◽  
Detection System ◽  
Microbial Detection

Validation of a microbial detection system for use with ACD-A platelets with PAS III platelet additive solution

Transfusion ◽  
2011 ◽  
Vol 51 (10) ◽  
pp. 2219-2227 ◽  
Author(s):  
Larry J. Dumont ◽  
Shauna N. Hay ◽  
Louise Herschel ◽  
Barbara Brantigan ◽  
Jaime Houghton ◽  
...  
Keyword(s):  
Detection System ◽  
Microbial Detection ◽  
Additive Solution

Evaluation of the Bactec microbial detection system for culturing miscellaneous sterile body fluids

2006 ◽  
Vol 157 (5) ◽  
pp. 433-436 ◽  
Author(s):  
Fusun Zeynep Akcam ◽  
Guler Yayli ◽  
Ersin Uskun ◽  
Onur Kaya ◽  
Canan Demir
Keyword(s):  
Detection System ◽  
Body Fluids ◽  
Microbial Detection ◽  
Sterile Body Fluids

Two Cases of Recovery of Dimorphic Pathogenic Fungi via Conventional BacT/ALERT® Microbial Detection System Media

2008 ◽  
Vol 167 (4) ◽  
pp. 191-195
Author(s):  
Erik Munson ◽  
Tina Endes ◽  
Katharine Vaughan ◽  
Timothy Block ◽  
Jeanne E. Hryciuk
Keyword(s):  
Detection System ◽  
Pathogenic Fungi ◽  
Microbial Detection

scholarly journals Effect of delayed entry on performance of the BACT/ALERT FAN PLUS bottles in the BACT/ALERT VIRTUO blood culture system

2020 ◽  
Author(s):  
Mary Adamik ◽  
Anne Hutchins ◽  
Jasmin Mangilit ◽  
Betsy Katzin ◽  
Heather Totty ◽  
...  
Keyword(s):  
Blood Culture ◽  
Room Temperature ◽  
Detection System ◽  
Blood Culture System ◽  
Microbial Detection ◽  
Delayed Entry ◽  
Colony Forming Units ◽  
Delay Times ◽  
Time To Detection ◽  
Mean Time

Abstract Delayed entry of patient blood culture samples into a microbial detection system is unavoidable at times, due to off-shift staffing or transporting samples to centralized laboratories. Pre-incubation time and temperature of blood culture bottles are the most critical factors impacting recovery and detection of microorganisms. A total of 1377 BACT/ALERT® (BTA) Fastidious Antimicrobial Neutralization (FAN® PLUS) bottles (FA PLUS, FN PLUS, and PF PLUS) were tested after delayed entry times of 24 and 36 h at 20–25 °C (room temperature, RT) prior to loading into the BACT/ALERT® VIRTUO® microbial detection system (VIRTUO). Clinically relevant organisms were inoculated into bottles with 5–84 colony forming units (CFU) per bottle, and human blood (0 to 10 mL), and then loaded into the VIRTUO. When bottles were loaded without delay, a mean time to detection (TTD) of 9.6 h was observed. For delayed bottles, the TTD reported by the VIRTUO was added to the 24-h and 36-h delay times and resulted in average time to results of 32.5 h and 42.5 h, respectively. The FAN PLUS bottles in conjunction with the VIRTUO produced acceptable results when delays up to 24 h at 20–25 °C occur in loading.

A novel method to enable PCR testing of bacterial isolates in the BacT/ALERT Microbial Detection System

Pathology ◽  
2017 ◽  
Vol 49 (6) ◽  
pp. 656-658 ◽  
Author(s):  
Christopher J. McIver ◽  
Noel Er ◽  
Robert Stevens ◽  
Peter C. Taylor
Keyword(s):  
Detection System ◽  
Bacterial Isolates ◽  
Microbial Detection ◽  
Novel Method

UHV ENFINA - A New High-Performance EELS Spectrometer for the VG STEM

2001 ◽  
Vol 7 (S2) ◽  
pp. 1132-1133
Author(s):  
John A. Hunt ◽  
Frank E. Dickerson ◽  
A. Abbott ◽  
Gabriel Szantai ◽  
Paul E. Mooney
Keyword(s):  
Electron Microscope ◽  
Point Spread Function ◽  
High Performance ◽  
Detection System ◽  
Detector System ◽  
Thermo Electric ◽  
Point Spread ◽  
Spread Function ◽  
Readout Noise ◽  
New Generation

The Gatan Enfina EELS spectrometer, featuring a new generation of detection system, was recently developed to upgrade or replace Gatan PEELS / DigiPEELS systems. Major advantages of the system include significant improvements to the detector point spread function, readout speed, sensitivity, readout noise, and increased number of detection channels. This design was until now not available for VG STEM systems because compatible detectors must not significantly degrade the UHV column vacuum and must survive column baking. in this abstract we report on a new detector system with comparable specifications to the standard Enfina system but designed for new or for upgrading existing Gatan UHV PEELS / DigiPEELS systems on the VG STEM.The standard Enfina design has the scintillator, fibers, CCD, CCD socket board, flex-cables, thermo electric cooler and coupling grease in the shared vacuum of the EELS spectrometer and the electron microscope.

Sign in / Sign up

Export Citation Format

Share Document