Leptin induces tissue factor expression in human peripheral blood mononuclear cells: a possible link between obesity and cardiovascular risk?

2007 ◽  
Vol 5 (7) ◽  
pp. 1462-1468 ◽  
Author(s):  
E. NAPOLEONE ◽  
A. DI SANTO ◽  
C. AMORE ◽  
G. BACCANTE ◽  
C. DI FEBBO ◽  
...  
Keyword(s):  
Cardiovascular Risk ◽  
Tissue Factor ◽  
Peripheral Blood Mononuclear Cells ◽  
Peripheral Blood ◽  
Mononuclear Cells ◽  
Human Peripheral Blood ◽  
Tissue Factor Expression ◽  
Peripheral Blood Mononuclear ◽  
Blood Mononuclear Cells ◽  
Factor Expression

Echinomycin Suppresses the Pyrogenic Effects of Endotoxin and Interleukin- lβ in Human Endothelial Cells and Peripheral Blood Mononuclear Cells

1996 ◽  
Vol 75 (02) ◽  
pp. 359-362 ◽  
Author(s):  
Jean-Marc Herbert ◽  
Alain Lalé ◽  
Jean-Marie Pereillo ◽  
Jean-Marie Derocq ◽  
Pierre Casellas
Keyword(s):  
Endothelial Cells ◽  
Tissue Factor ◽  
Peripheral Blood Mononuclear Cells ◽  
Peripheral Blood ◽  
Mononuclear Cells ◽  
Human Peripheral Blood ◽  
Intercellular Adhesion Molecule ◽  
Dependent Manner ◽  
Peripheral Blood Mononuclear ◽  
Blood Mononuclear Cells

SummaryEndotoxin (LPS) and interleukin-1(3 (IL-iβ) increased in a dose-dependent manner the expression of tissue factor, an ubiquitous membrane-anchored glycoprotein that initiates blood coagulation at the surface of human umbilical vein endothelial cells (HUVEC) and human peripheral blood mononuclear cells (PBMC). Echinomycin, a cyclic octapeptide of microbial origin strongly inhibited LPS- and IL-1β-induced tissue factor expression in HUVEC and PBMC with IC50 values in the subnanomolar range at the same time it reduced LPS and IL-iβ-induced expression of intercellular adhesion molecule-1 (ICAM-1, CD54) on HUVEC (IC50 = 0.4 ± 0.1 and 0.3 ± 0.2 nM respectively). Echinomycin also reduced LPS-induced secretion of IL-iβ and IL-6 by human PBMC (IC50 = 10 ± 2 and 3 ± 0.5 nM respectively). These observations demonstrate that echinomycin protects endothelial cells and PBMC from the pyrogenic effect of LPS and 1β.

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