Identification and Partial Purification of a Stage-Specific 33 kDa Mitochondrial Protein as the Alternative Oxidase of the Trypanosoma brucei brucei Bloodstream Trypomastigotes

1995 ◽  
Vol 42 (5) ◽  
pp. 467-472 ◽  
Author(s):  
MINU CHAUDHURI ◽  
WILFRED AJAYI ◽  
STARLETT TEMPLE ◽  
GEORGE C. HILL
Keyword(s):  
Trypanosoma Brucei ◽  
Alternative Oxidase ◽  
Mitochondrial Protein ◽  
Partial Purification ◽  
Trypanosoma Brucei Brucei

Purification of sn-glycerol-3-phosphate dehydrogenase from Trypanosoma brucei brucei

1992 ◽  
Vol 70 (2) ◽  
pp. 136-141 ◽  
Author(s):  
Jack A. Kornblatt ◽  
Joseph Nthale ◽  
Francis McOdimba
Keyword(s):  
Electron Transfer ◽  
Molecular Mass ◽  
Trypanosoma Brucei ◽  
Alternative Oxidase ◽  
Polyacrylamide Gel Electrophoresis ◽  
Sodium Dodecyl ◽  
Trypanosoma Brucei Brucei ◽  
Subsequent Formation ◽  
Glycerol 3 Phosphate Dehydrogenase ◽  
Trypanosome Alternative Oxidase

A protein has been purified from the membranes of bloodstream forms of Trypanosoma brucei brucei. The purified material contained a single polypeptide chain of molecular mass 67 kilodaltons as judged by sodium dodecyl sulfate –polyacrylamide gel electrophoresis; under "native" conditions it migrated through a Sephacryl S-300 column with a similar molecular mass. The purified protein catalysed electron transfer from sn-glycerol 3-phosphate to oxygen with the subsequent formation of water. Electron transfer by the purified enzyme to O2 was dependent on the presence of low concentrations of the mediator phenazine methosulfate. This protein is clearly the major membrane-bound sn-glycerol-3-phosphate dehydrogenase, but it also has some characteristics suggestive of the trypanosome alternative oxidase activities.Key words: trypanosomes, glycerophosphate dehydrogenase, trypanosome alternative oxidase.

scholarly journals Discovery of trypanocidal coumarins with dual inhibition of both the glycerol kinase and alternative oxidase of Trypanosoma brucei brucei

2019 ◽  
Vol 33 (11) ◽  
pp. 13002-13013 ◽  
Author(s):  
Emmanuel Oluwadare Balogun ◽  
Daniel Ken Inaoka ◽  
Tomoo Shiba ◽  
Chiaki Tsuge ◽  
Benjamin May ◽  
...  
Keyword(s):  
Trypanosoma Brucei ◽  
Alternative Oxidase ◽  
Glycerol Kinase ◽  
Trypanosoma Brucei Brucei ◽  
Dual Inhibition

scholarly journals Purification and kinetic characterization of recombinant alternative oxidase from Trypanosoma brucei brucei

2010 ◽  
Vol 1797 (4) ◽  
pp. 443-450 ◽  
Author(s):  
Yasutoshi Kido ◽  
Kimitoshi Sakamoto ◽  
Kosuke Nakamura ◽  
Michiyo Harada ◽  
Takashi Suzuki ◽  
...  
Keyword(s):  
Trypanosoma Brucei ◽  
Alternative Oxidase ◽  
Kinetic Characterization ◽  
Trypanosoma Brucei Brucei

Novel inhibitors of the trypanosome alternative oxidase inhibit Trypanosoma brucei brucei growth and respiration

Acta Tropica ◽  
2006 ◽  
Vol 100 (3) ◽  
pp. 172-184 ◽  
Author(s):  
Robert Ott ◽  
Kelly Chibale ◽  
Sedrick Anderson ◽  
Alex Chipeleme ◽  
Minu Chaudhuri ◽  
...  
Keyword(s):  
Trypanosoma Brucei ◽  
Alternative Oxidase ◽  
Trypanosoma Brucei Brucei ◽  
Trypanosome Alternative Oxidase
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