scholarly journals Molecular characterization of a flagellar/chemotaxis operon in the spirochete Borrelia burgdorferi

2006 ◽  
Vol 153 (2) ◽  
pp. 425-431 ◽  
Author(s):  
Yigong Ge ◽  
Nyles W Charon
1999 ◽  
Vol 181 (23) ◽  
pp. 7308-7313 ◽  
Author(s):  
Christian H. Eggers ◽  
D. Scott Samuels

ABSTRACT We have recovered a DNase-protected, chloroform-resistant molecule of DNA from the cell-free supernatant of a Borrelia burgdorferi culture. The DNA is a 32-kb double-stranded linear molecule that is derived from the 32-kb circular plasmids (cp32s) of the B. burgdorferi genome. Electron microscopy of samples from which the 32-kb DNA molecule was purified revealed bacteriophage particles. The bacteriophage has a polyhedral head with a diameter of 55 nm and appears to have a simple 100-nm-long tail. The phage is produced constitutively at low levels from growing cultures of someB. burgdorferi strains and is inducible to higher levels with 10 μg of 1-methyl-3-nitroso-nitroguanidine (MNNG) ml−1. In addition, the prophage can be induced with MNNG from some Borrelia isolates that do not naturally produce phage. We have isolated and partially characterized the phage associated with B. burgdorferi CA-11.2A. To our knowledge, this is the first molecular characterization of a bacteriophage ofB. burgdorferi.


2007 ◽  
Vol 38 (1) ◽  
pp. 14-16 ◽  
Author(s):  
Elizabeta S. Ristanovic ◽  
Kayoko Kitamura ◽  
Toshiyuki Masuzawa ◽  
Marija J. Milutinovic ◽  
Radovan M. Cekanac ◽  
...  

1992 ◽  
Vol 24 (2) ◽  
pp. 181-188 ◽  
Author(s):  
Sven Bergström ◽  
BjÖRn Olsén ◽  
Nils Burman ◽  
Leif Gothefors ◽  
Thomas G.T. Jaenson ◽  
...  

2003 ◽  
Vol 41 (4) ◽  
pp. 1569-1573 ◽  
Author(s):  
J. W. Courtney ◽  
R. L. Dryden ◽  
J. Montgomery ◽  
B. S. Schneider ◽  
G. Smith ◽  
...  

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