scholarly journals Cloning, nucleotide sequence and expression of the gene encoding the cellulose-binding protein A (CBPA) ofEubacterium cellulosolvens5

2002 ◽  
Vol 207 (2) ◽  
pp. 141-146 ◽  
Author(s):  
Atsushi Toyoda ◽  
Hajime Minato
Keyword(s):  
Nucleotide Sequence ◽  
Binding Protein ◽  
Protein A ◽  
Gene Encoding ◽  
Cellulose Binding

Cloning, nucleotide sequence and expression of the gene encoding the cellulose-binding protein 1 (CBP1) ofFibrobacter succinogenesS85

1996 ◽  
Vol 139 (1) ◽  
pp. 43-50 ◽  
Author(s):  
Makoto Mitsumori ◽  
Hajime Minato ◽  
Tsutomu Sekizaki ◽  
Ikuo Uchida ◽  
Hiroya Ito
Keyword(s):  
Nucleotide Sequence ◽  
Binding Protein ◽  
Gene Encoding ◽  
Cellulose Binding

Nucleotide sequence ofCab-8, a new type I gene encoding a chlorophylla/b-binding protein of LHC II inPisum

1991 ◽  
Vol 17 (3) ◽  
pp. 523-526 ◽  
Author(s):  
Laura Alexander ◽  
Denis Falconet ◽  
Brian W. Fristensky ◽  
Michael J. White ◽  
John C. Watson ◽  
...  
Keyword(s):  
Nucleotide Sequence ◽  
Binding Protein ◽  
Type I ◽  
Gene Encoding ◽  
Lhc Ii ◽  
New Type ◽  
I Gene

scholarly journals Characterization of GTPase Activity of TrmE, a Member of a Novel GTPase Superfamily, from Thermotoga maritima

2000 ◽  
Vol 182 (24) ◽  
pp. 7078-7082 ◽  
Author(s):  
Kunitoshi Yamanaka ◽  
Jihwan Hwang ◽  
Masayori Inouye
Keyword(s):  
Thermotoga Maritima ◽  
Binding Protein ◽  
Protein A ◽  
Hydrolysis Rate ◽  
Gtpase Activity ◽  
Gtp Binding Protein ◽  
Gene Encoding ◽  
Gtp Hydrolysis ◽  
Gtp Binding

ABSTRACT A gene encoding a putative GTP-binding protein, a TrmE homologue that is highly conserved in both prokaryotes and eukaryotes, was cloned from Thermotoga maritima, a hyperthermophilic bacterium.T. maritima TrmE was overexpressed in Escherichia coli and purified. TrmE has a GTPase activity but no ATPase activity. The GTPase activity can be competed with GTP, GDP, and dGTP but not with GMP, ATP, CTP, or UTP. Km andk cat at 70°C were 833 μM and 9.3 min−1, respectively. Our results indicate that TrmE is a GTP-binding protein with a very high intrinsic GTP hydrolysis rate. We also propose that TrmE homologues constitute a novel subfamily of the GTPase superfamily.

scholarly journals Characterization of the cellulose-binding domain of the Clostridium cellulovorans cellulose-binding protein A.

1993 ◽  
Vol 175 (18) ◽  
pp. 5762-5768 ◽  
Author(s):  
M A Goldstein ◽  
M Takagi ◽  
S Hashida ◽  
O Shoseyov ◽  
R H Doi ◽  
...  
Keyword(s):  
Binding Protein ◽  
Protein A ◽  
Binding Domain ◽  
Cellulose Binding Domain ◽  
Clostridium Cellulovorans ◽  
Cellulose Binding
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