scholarly journals Identification of the genusGeobacillususing genus-specific primers, based on the 16S–23S rRNA gene internal transcribed spacer

2007 ◽  
Vol 277 (2) ◽  
pp. 165-172 ◽  
Author(s):  
Nomeda Kuisiene ◽  
Juozas Raugalas ◽  
Milda Stuknyte ◽  
Donaldas Chitavichius
Keyword(s):  
Internal Transcribed Spacer ◽  
23S Rrna ◽  
Rrna Gene ◽  
Specific Primers ◽  
23S Rrna Gene

scholarly journals 16S–23S rRNA gene internal transcribed spacer sequences for analysis of the phylogenetic relationships among species of the genus Porphyromonas

2005 ◽  
Vol 55 (2) ◽  
pp. 607-613 ◽  
Author(s):  
Georg Conrads ◽  
Diane M. Citron ◽  
Kerin L. Tyrrell ◽  
Hans-Peter Horz ◽  
Ellie J. C. Goldstein
Keyword(s):  
Internal Transcribed Spacer ◽  
Type Strain ◽  
Distinct Species ◽  
Genetic Identification ◽  
23S Rrna ◽  
Rrna Gene ◽  
23S Rrna Gene ◽  
Internal Transcribed Spacer Sequences ◽  
Tannerella Forsythensis ◽  
Reference Strains

The 16S–23S rRNA gene internal transcribed spacer (ITS) regions of 11 reference strains of Porphyromonas species, together with Bacteroides distasonis and Tannerella forsythensis, were analysed to examine interspecies relationships. Compared with the phylogenetic tree generated using 16S rRNA gene sequences, the resolution of the ITS sequence-based tree was higher, but species positioning and clustering were similar with both approaches. The recent separation of Porphyromonas gulae and Porphyromonas gingivalis into distinct species was confirmed by the ITS data. In addition, analysis of the ITS sequences of 24 clinical isolates of Porphyromonas asaccharolytica plus the type strain ATCC 25260T divided the sequences into two clusters, of which one was α-fucosidase-positive (like the type strain) while the other was α-fucosidase-negative. The latter resembled the previously studied unusual extra-oral isolates of ‘Porphyromonas endodontalis-like organisms' (PELOs) which could therefore be called ‘Porphyromonas asaccharolytica-like organisms' (PALOs), based on the genetic identification. Moreover, the proposal of α-fucosidase-negative P. asaccharolytica strains as a new species should also be considered.

scholarly journals Differentiation between Mycobacterium tuberculosis and Mycobacterium avium by Amplification of the 16S-23S Ribosomal DNA Spacer

1998 ◽  
Vol 36 (9) ◽  
pp. 2399-2403 ◽  
Author(s):  
Arunnee Sansila ◽  
Poonpilas Hongmanee ◽  
Charoen Chuchottaworn ◽  
Somsak Rienthong ◽  
Dhanida Rienthong ◽  
...  
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Mycobacterial Infection ◽  
Clinical Isolates ◽  
23S Rrna ◽  
Enzyme Analysis ◽  
Restriction Enzyme Analysis ◽  
Rrna Gene ◽  
Biochemical Tests ◽  
Specific Primers ◽  
23S Rrna Gene

Differentiation between Mycobacterium tuberculosis andM. avium is helpful for the treatment of disseminated mycobacterial infection in AIDS patients. This can traditionally be done by time-consuming biochemical tests or with Accuprobe. Previously, PCR restriction enzyme analysis (PCR-REA) of the 16S-23S rRNA gene spacer was shown to be able to identify a limited number of strains ofMycobacterium. In this study the method was improved by using more specific primers and was tested with 50 clinical isolates ofM. tuberculosis and 65 clinical isolates of M. avium complex. Probes specific to the spacers ofM. tuberculosis and M. avium were also tested. Both M. tuberculosis and M. avium could be reliably identified either by PCR-REA or by PCR-hybridization, with the results completely agreeing with those obtained by biochemical tests and with the Accuprobe, respectively. The method may therefore be useful as an alternative in-house method for identification of the bacteria.

Species‐specific Identification of Vibrio sp. based on 16S‐23S rRNA gene internal transcribed spacer

2020 ◽  
Vol 129 (3) ◽  
pp. 738-752
Author(s):  
J. Yu ◽  
B. Zhu ◽  
T. Zhou ◽  
Y. Wei ◽  
X. Li ◽  
...  
Keyword(s):  
Internal Transcribed Spacer ◽  
23S Rrna ◽  
Rrna Gene ◽  
Specific Identification ◽  
23S Rrna Gene ◽  
Species Specific

scholarly journals Molecular detection and evaluation of resistance of Mycoplasma Pneumoniae to macrolide (Erythromycin) due to mutation in the 23S rRNA gene among Iranian Patients with respiratory infections

2020 ◽  
Author(s):  
Mohammad Niakan ◽  
Susan Rostampur ◽  
Reza Mirnejad ◽  
Mehrdad Halaji ◽  
Iman Pouladi
Keyword(s):  
Mycoplasma Pneumoniae ◽  
Molecular Detection ◽  
Inhibitory Concentration ◽  
Respiratory Infections ◽  
Community Acquired Pneumonia ◽  
23S Rrna ◽  
Rrna Gene ◽  
Specific Primers ◽  
23S Rrna Gene ◽  
Iranian Patients

Abstract Objective: Mycoplasma pneumoniae is a common cause of community-acquired pneumonia. The global increased resistance of M. pneumoniae strains to macrolide (ML) has become a worrisome health problem. The widespread use of these drugs has led to increased rate of reported ML-resistant M. pneumoniae (MRMP) throughout the world. Therefore, this study was aimed to evaluate the resistance of M. pneumoniae against erythromycin due to mutations in the 23S rRNA gene of patients with respiratory infections in Iran.Results: According to the findings of the present study, employing specific primers showed that 17 cases (17%) were positive for mycoplasma genus and 6 cases (6%) positive for M. pneumoniae species. Also, analysis of the sequence of 23S rRNA gene, revealed that one of the samples had mutations at positions A2431G and G2491A. Measuring the minimum inhibitory concentration (MIC), revealed that all samples positive for M. pneumoniae with 23S rRNA gene were sensitive to erythromycin, and no ML resistance was reported.

scholarly journals The Internal Transcribed Spacer Region, a New Tool for Use in Species Differentiation and Delineation of Systematic Relationships within the Campylobacter Genus

2010 ◽  
Vol 76 (10) ◽  
pp. 3071-3081 ◽  
Author(s):  
Si Ming Man ◽  
Nadeem O. Kaakoush ◽  
Sophie Octavia ◽  
Hazel Mitchell
Keyword(s):  
16S Rrna ◽  
Internal Transcribed Spacer ◽  
Its Region ◽  
Rrna Genes ◽  
23S Rrna ◽  
Species Differentiation ◽  
Rrna Gene ◽  
23S Rrna Gene ◽  
Systematic Relationships ◽  
23S Rrna Genes

ABSTRACT The Campylobacter genus consists of a number of important human and animal pathogens. Although the 16S rRNA gene has been used extensively for detection and identification of Campylobacter species, there is currently limited information on the 23S rRNA gene and the internal transcribed spacer (ITS) region that lies between the 16S and 23S rRNA genes. We examined the potential of the 23S rRNA gene and the ITS region to be used in species differentiation and delineation of systematic relationships for 30 taxa within the Campylobacter genus. The ITS region produced the highest mean pairwise percentage difference (35.94%) compared to the 16S (5.34%) and 23S (7.29%) rRNA genes. The discriminatory power for each region was further validated using Simpson's index of diversity (D value). The D values were 0.968, 0.995, and 0.766 for the ITS region and the 23S and 16S rRNA genes, respectively. A closer examination of the ITS region revealed that Campylobacter concisus, Campylobacter showae, and Campylobacter fetus subsp. fetus harbored tRNA configurations not previously reported for other members of the Campylobacter genus. We also observed the presence of strain-dependent intervening sequences in the 23S rRNA genes. Neighbor-joining trees using the ITS region revealed that Campylobacter jejuni and Campylobacter coli strains clustered in subgroups, which was not observed in trees derived from the 16S or 23S rRNA gene. Of the three regions examined, the ITS region is by far the most cost-effective region for the differentiation and delineation of systematic relationships within the Campylobacter genus.

Sign in / Sign up

Export Citation Format

Share Document