In vitro characterization of three-dimensional scaffolds seeded with human bone marrow stromal cells for tissue engineered growth of bone: mission impossible? A methodological approach

2008 ◽  
Vol 19 (4) ◽  
pp. 379-386 ◽  
Author(s):  
Thomas Materna ◽  
Hans J. Rolf ◽  
Johanna Napp ◽  
Jutta Schulz ◽  
Michael Gelinsky ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Stromal Cells ◽  
Bone Marrow Stromal Cells ◽  
Methodological Approach ◽  
Three Dimensional ◽  
Marrow Stromal Cells ◽  
Human Bone ◽  
In Vitro Characterization

scholarly journals In vitro characterization of bone marrow stromal cells from osteoarthritic donors

2016 ◽  
Vol 16 (3) ◽  
pp. 782-789 ◽  
Author(s):  
Maik Stiehler ◽  
Juliane Rauh ◽  
Cody Bünger ◽  
Angela Jacobi ◽  
Corina Vater ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Stromal Cells ◽  
Bone Marrow Stromal Cells ◽  
Marrow Stromal Cells ◽  
In Vitro Characterization

scholarly journals Human Bone Marrow Stromal Cells: A Reliable, Challenging Tool forIn VitroOsteogenesis and Bone Tissue Engineering Approaches

2016 ◽  
Vol 2016 ◽  
pp. 1-14 ◽  
Author(s):  
Ute Hempel ◽  
Katrin Müller ◽  
Carolin Preissler ◽  
Carolin Noack ◽  
Sabine Boxberger ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Osteogenic Differentiation ◽  
Stromal Cells ◽  
Bone Marrow Stromal Cells ◽  
Marrow Stromal Cells ◽  
Human Bone ◽  
Human Bone Marrow ◽  
Peroxisome Proliferator ◽  
Differentiation Potential

Adult human bone marrow stromal cells (hBMSC) are important for many scientific purposes because of their multipotency, availability, and relatively easy handling. They are frequently used to study osteogenesisin vitro. Most commonly, hBMSC are isolated from bone marrow aspirates collected in clinical routine and cultured under the “aspect plastic adherence” without any further selection. Owing to the random donor population, they show a broad heterogeneity. Here, the osteogenic differentiation potential of 531 hBMSC was analyzed. The data were supplied to correlation analysis involving donor age, gender, and body mass index. hBMSC preparations were characterized as follows: (a) how many passages the osteogenic characteristics are stable in and (b) the influence of supplements and culture duration on osteogenic parameters (tissue nonspecific alkaline phosphatase (TNAP), octamer binding transcription factor 4, core-binding factor alpha-1, parathyroid hormone receptor, bone gla protein, and peroxisome proliferator-activated proteinγ). The results show that no strong prediction could be made from donor data to the osteogenic differentiation potential; only the ratio of induced TNAP to endogenous TNAP could be a reliable criterion. The results give evidence that hBMSC cultures are stable until passage 7 without substantial loss of differentiation potential and that established differentiation protocols lead to osteoblast-like cells but not to fully authentic osteoblasts.

Potential of Human Bone Marrow Stromal Cells to Accelerate Wound Healing in Vitro

2005 ◽  
Vol 55 (4) ◽  
pp. 414-419 ◽  
Author(s):  
Seung-Kyu Han ◽  
Tae-Hwan Yoon ◽  
Dong-Geun Lee ◽  
Min-Ah Lee ◽  
Woo-Kyung Kim
Keyword(s):  
Bone Marrow ◽  
Wound Healing ◽  
Stromal Cells ◽  
Bone Marrow Stromal Cells ◽  
Marrow Stromal Cells ◽  
Human Bone ◽  
Human Bone Marrow ◽  
Accelerate Wound Healing
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