In this study, the oxygen contents of the indoor air quality in different climatic conditions in a chamber were real-time monitored. At the same time, ten young volunteers sit in the three different environment conditions to fill subject feeling questionnaires. The climatic conditions referred to three groups of hot and humid environment (30°C, 35°C, 40°Cdry bulb temperature and 90% relative humidity). The results reveal the oxygen content of indoor air quality slightly decreased in different thermal environments, the values within normal variations. Questionnaire statistic results indicate that the temperature and humidity control in air conditioning significantly influence the human feelings. Thirsty, head heavy, tired, irritability, distraction, chest tightness and cold sweating are the main emerging symptoms in such indoor environments. Reasonable control of indoor temperature and humidity has vital importance to indoor environment quality.