Epithelial-mesenchymal interactions induce enamel matrix proteins and proteases in the epithelial cells of the rests of Malassez in vitro

2012 ◽  
Vol 120 (6) ◽  
pp. 475-483 ◽  
Author(s):  
Ken Takahashi ◽  
Mitsuru Shimonishi ◽  
Rui Wang ◽  
Hiroatsu Watanabe ◽  
Masahiko Kikuchi
Keyword(s):  
Epithelial Cells ◽  
Matrix Proteins ◽  
Enamel Matrix ◽  
Enamel Matrix Proteins

scholarly journals Effects of enamel matrix proteins on adherence, proliferation and migration of epithelial cells: A real-time in vitro study

2016 ◽  
Vol 13 (1) ◽  
pp. 160-168 ◽  
Author(s):  
Marzena Wyganowska-Swiatkowska ◽  
Paulina Urbaniak ◽  
Daniel Lipinski ◽  
Marlena Szalata ◽  
Karolina Borysiak ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Real Time ◽  
In Vitro Study ◽  
Vitro Study ◽  
Matrix Proteins ◽  
Enamel Matrix ◽  
Enamel Matrix Proteins ◽  
And Migration ◽  
Proliferation And Migration

Effects of enamel matrix proteins on multi-lineage differentiation of periodontal ligament cells in vitro

2013 ◽  
Vol 9 (1) ◽  
pp. 4796-4805 ◽  
Author(s):  
Harsh D. Amin ◽  
Irwin Olsen ◽  
Jonathan C. Knowles ◽  
Michel Dard ◽  
Nikolaos Donos
Keyword(s):  
Periodontal Ligament ◽  
Matrix Proteins ◽  
Periodontal Ligament Cells ◽  
Enamel Matrix ◽  
Enamel Matrix Proteins ◽  
Lineage Differentiation

scholarly journals Protein nanoribbons template enamel mineralization

2020 ◽  
Vol 117 (32) ◽  
pp. 19201-19208 ◽  
Author(s):  
Yushi Bai ◽  
Zanlin Yu ◽  
Larry Ackerman ◽  
Yan Zhang ◽  
Johan Bonde ◽  
...  
Keyword(s):  
Supramolecular Assembly ◽  
Organic Content ◽  
Matrix Proteins ◽  
Maturation Stage ◽  
Enamel Matrix ◽  
Structural Protein ◽  
Enamel Matrix Proteins ◽  
Tissue Hardness ◽  
Enamel Mineralization

As the hardest tissue formed by vertebrates, enamel represents nature’s engineering masterpiece with complex organizations of fibrous apatite crystals at the nanometer scale. Supramolecular assemblies of enamel matrix proteins (EMPs) play a key role as the structural scaffolds for regulating mineral morphology during enamel development. However, to achieve maximum tissue hardness, most organic content in enamel is digested and removed at the maturation stage, and thus knowledge of a structural protein template that could guide enamel mineralization is limited at this date. Herein, by examining a gene-modified mouse that lacked enzymatic degradation of EMPs, we demonstrate the presence of protein nanoribbons as the structural scaffolds in developing enamel matrix. Using in vitro mineralization assays we showed that both recombinant and enamel-tissue–based amelogenin nanoribbons are capable of guiding fibrous apatite nanocrystal formation. In accordance with our understanding of the natural process of enamel formation, templated crystal growth was achieved by interaction of amelogenin scaffolds with acidic macromolecules that facilitate the formation of an amorphous calcium phosphate precursor which gradually transforms into oriented apatite fibers along the protein nanoribbons. Furthermore, this study elucidated that matrix metalloproteinase-20 is a critical regulator of the enamel mineralization as only a recombinant analog of a MMP20-cleavage product of amelogenin was capable of guiding apatite mineralization. This study highlights that supramolecular assembly of the scaffold protein, its enzymatic processing, and its ability to interact with acidic carrier proteins are critical steps for proper enamel development.

In vitro Effects of Enamel Matrix Proteins on Rat Bone Marrow Cells and Gingival Fibroblasts

2004 ◽  
Vol 83 (2) ◽  
pp. 134-138 ◽  
Author(s):  
S. Keila ◽  
C.E. Nemcovsky ◽  
O. Moses ◽  
Z. Artzi ◽  
M. Weinreb
Keyword(s):  
Bone Marrow ◽  
Fold Increase ◽  
Matrix Proteins ◽  
Enamel Matrix ◽  
Gingival Fibroblasts ◽  
Enamel Matrix Proteins ◽  
Alp Activity ◽  
In Vitro Effects ◽  
Rat Bone

Emdogain® (EMD), a formulation of Enamel Matrix Proteins (EMP), is used clinically for periodontal regeneration, where it stimulates cementum formation and promotes gingival healing. In this study, we investigated the in vitro effects of EMD on rat bone marrow stromal cells (BMSC) and gingival fibroblasts (GF). EMD (at 25 μg/mL) increased the osteogenic capacity of bone marrow, as evidenced by ~ three-fold increase in BMSC cell number and ~ two-fold increase in alkaline phosphatase (ALP) activity and mineralized nodule formation. The presence of EMD in the initial stages (first 48 hrs) of the culture was crucial for this effect. In contrast, EMD did not induce osteoblastic differentiation of GF (evidenced by lack of mineralization or ALP activity) but increased up to two-fold both their number and the amount of matrix produced. These in vitro data on BMSC and GF could explain the promotive effect of EMD on bone formation and connective tissue regeneration, respectively.

Expression Profiling of Periodontal Ligament Cells Stimulated with Enamel Matrix Proteins in vitro: A Model for Tissue Regeneration

2002 ◽  
Vol 81 (11) ◽  
pp. 776-783 ◽  
Author(s):  
P.M. Brett ◽  
M. Parkar ◽  
I. Olsen ◽  
M. Tonetti
Keyword(s):  
Periodontal Ligament ◽  
Gene Array ◽  
Periodontal Regeneration ◽  
Matrix Proteins ◽  
Periodontal Ligament Cells ◽  
Enamel Matrix ◽  
Enamel Matrix Proteins ◽  
Genetic Responses

Several studies have examined the role of enamel matrix proteins in root formation and periodontal regeneration, although most of these have focused on a few specific genes which had previously been implicated. However, recent advances in expressional profiling have made it possible to examine the range of genetic responses involved in these processes. In the present experiments, we have therefore utilized this technique to determine the effects of enamel matrix proteins on the gene activities of periodontal ligament cells in vitro. Such cells were found to have an elevated level of RNA synthesis compared with control cells. Moreover, hybridization of the cDNA prepared from this RNA to gene array filters showed that there was differential expression of 121 genes, most of which had not previously been associated with periodontal regeneration. Some of these selective changes in gene activity might thus reflect the fundamental events that underlie periodontal development.

Modulation of enamel matrix proteins on the formation and nano-assembly of hydroxyapatite in vitro

2012 ◽  
Vol 32 (4) ◽  
pp. 858-861 ◽  
Author(s):  
Hong Li ◽  
Weiya Huang ◽  
Yuanming Zhang ◽  
Bo Xue ◽  
Xuejun Wen
Keyword(s):  
Matrix Proteins ◽  
Enamel Matrix ◽  
Enamel Matrix Proteins
Sign in / Sign up

Export Citation Format

Share Document