Characterization of T lymphocyte clones derived from Porphyromonas gingivalis infected subjects

1996 ◽  
Vol 31 (1) ◽  
pp. 47-56 ◽  
Author(s):  
E. Gemmell ◽  
V. Woodford ◽  
G.J. Seymour
Keyword(s):  
Porphyromonas Gingivalis ◽  
T Lymphocyte

Flow cytometric quantitation and characterization of the T-lymphocyte memory response to CMV in healthy donors

Cytotherapy ◽  
2002 ◽  
Vol 4 (1) ◽  
pp. 29-40 ◽  
Author(s):  
N. Hensel ◽  
J.J. Melenhorst ◽  
K. Bradstock ◽  
A.P. Schwarer ◽  
R. Eniafe ◽  
...  
Keyword(s):  
T Lymphocyte ◽  
Memory Response ◽  
Flow Cytometric ◽  
Healthy Donors

scholarly journals Antigen-specific T lymphocyte clones. I. Characterization of a T lymphocyte clone expressing antigen-specific suppressive activity.

1981 ◽  
Vol 153 (5) ◽  
pp. 1246-1259 ◽  
Author(s):  
M Fresno ◽  
G Nabel ◽  
L McVay-Boudreau ◽  
H Furthmayer ◽  
H Cantor
Keyword(s):  
T Cell ◽  
T Lymphocyte ◽  
Antigen Binding ◽  
Suppressive Activity ◽  
Cell Functions ◽  
Cell Clones ◽  
Helper Activity ◽  
Binding Peptides ◽  
Lymphocyte Clone

We have generated continuously propagatable T lymphocyte clones to study antigen-specific T cell functions. All Ly-2+ clones mediate suppressive activity and secrete a characteristic pattern of polypeptides that differs from Ly-2- T cell clones. Cells of one clone, Cl.Ly23/4, specifically bind glycophorin from sheep erythrocytes (SRBC). After incubation with [35S]methionine, supernate material from this clone also contains biosynthetically labeled 70,000-mol wt proteins that specifically bind to SRBC and this binding is inhibited by glycophorin from sheep but not other erythrocytes. These antigen-binding 70,000-mol wt peptides specifically and completely suppress primary anti-SRBC responses generated by mixtures of primed Ly-1+2- cells and B cells. Suppression by these antigen-binding peptides reflects direct inhibition of T-helper activity.

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