Electron microscope 3D reconstruction of branched collagen fibrilsin vivo

2009 ◽  
Vol 19 (4) ◽  
pp. 547-552 ◽  
Author(s):  
T. Starborg ◽  
Y. Lu ◽  
A. Huffman ◽  
D. F. Holmes ◽  
K. E. Kadler
Author(s):  
R. Hegerl ◽  
Z. Cejka ◽  
W. Baumeister

Two major questions concerning the results of 3D reconstruction are: 1) The effects of the interaction between specimen and specimen support and 2) the effects of stain level and stain distribution. These questions were approached using the S-layer of the bacterium Clostridium thermohydrosulphuricum which in the electron microscope is usually doubled upon itself. From these specimens independent 3D reconstructions of the upper and lower layers were made and compared with each other.


Author(s):  
O.H. Kapp ◽  
D.A. Crewe ◽  
A.V. Crewe

A serious limitation in the 3D reconstruction of aperiodic objects in the electron microscope is imposed by the difficulty in assigning the correct relative orientation to a population of molecules which may present themselves in any number of positions on the substrate. Additionally, some orientations may be found far more frequently than others. The hemoglobin of Lumbricus terrestris presents itself in only three or four easily recognizable positions in the EM. We have recently examined the possibility of inexact 3D reconstruction from a limited number of 2D projections and would like to apply this technique to this molecule using only the three most frequently found orientations (a ‘top’, ‘side’, and 45° diagonal) whose positions relative to one another can be interpreted with reasonable confidence.


2000 ◽  
Vol 40 (supplement) ◽  
pp. S168
Author(s):  
C. Sato ◽  
Y. Ueno ◽  
K. Takahashi ◽  
K. Asai ◽  
M. Sato ◽  
...  

2013 ◽  
Vol 23 (1) ◽  
pp. 168-174 ◽  
Author(s):  
A. A. Zolotukhin ◽  
I. V. Safonov ◽  
K. A. Kryzhanovskii

2005 ◽  
Author(s):  
Junghoon Lee ◽  
Yili Zheng ◽  
Peter C. Doerschuk ◽  
Jinghua Tang ◽  
John E. Johnson

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