B- AND T-CELLS AND INTRACELLULAR Ig-SYNTHESIS OF PERIPHERAL LYMPHOCYTES IN CHILDREN WITH ASTHMA AND/OR PREVIOUS ADENO-TONSILLECTOMY

2009 ◽  
Vol 85C (6) ◽  
pp. 454-462
Author(s):  
Poul Aabel ØStergaard
Keyword(s):  
T Cells ◽  
Peripheral Lymphocytes ◽  
Children With Asthma ◽  
Ig Synthesis

scholarly journals Human Germinal Center CD4+CD57+T Cells Act Differently On B Cells Than Do Classical T-Helper Cells

1995 ◽  
Vol 4 (3) ◽  
pp. 189-197 ◽  
Author(s):  
Farida Bouzahzah ◽  
Alain Bosseloir ◽  
Ernst Heinen ◽  
Léon J. Simar
Keyword(s):  
Cell Proliferation ◽  
T Cells ◽  
T Cell ◽  
B Cells ◽  
B Cell ◽  
Germinal Center ◽  
Functional Study ◽  
Target Cells ◽  
Helper Cells ◽  
Ig Synthesis

We have isolated two subtypes of helper T cells from human tonsils: CD4+CD57+cells, mostly located in the germinal center (GC), and CD4+CD57-cells, distributed through the interfollicular areas but also present in the GC. In a functional study, we have compared the capacities of these T-cell subtypes to stimulate B cells in cocultures. In order to block T-cell proliferation while maintaining their activation level, we pretreated isolated T cells with mitomycin C prior to culture in the presence of B cells and added polyclonal activators such as PHA and Con A, combined or not with IL-2. Contrary to CD4+CD57-cells, CD4+CD57+cells did not markedly enhance B-cell proliferation. Even when sIgD-B cells typical of germinal center cells were tested, the CD4 CD57 cells had no significant effect. This is in accordance with the location of these cells: They mainly occupy the light zones of the GC where few B cells divide. Even when added to preactivated, actively proliferating cells, CD4+CD57+cells failed to modulate B-cell multiplication. On the supernatants of B-cell-T-cell cocultures, we examined by the ELISA technique the effect of T cells on Ig synthesis. Contrary to CD57-T cells, whose effect was strong, CD57+T cells weakly stimulated Ig synthesis. More IgM than IgG was generally found. Because CD57 antigen is a typical marker of natural killer cells, we tested the cytolytic activity of tonsillar CD4+CD57+cells on K562 target cells. Unlike NK cells, neither CD4+CD57+nor CD4+CD57-cells exhibit any cytotoxicity. Thus, germinal center CD4+CD57+cells are not cytolytic and do not strongly stimulate either B-cell proliferation or Ig secretion. CD4+CD57-cells, however, enhance B-cell proliferation and differentiation, thus acting like the classical helper cells of the T-dependent areas.

scholarly journals Specificity and function of a human autologous reactive T cell.

1979 ◽  
Vol 149 (6) ◽  
pp. 1537-1542 ◽  
Author(s):  
P B Hausman ◽  
J D Stobo
Keyword(s):  
T Cells ◽  
B Cells ◽  
Human Peripheral Blood ◽  
Hla Dr ◽  
Helper Activity ◽  
Normal Human ◽  
Normal Human Peripheral Blood ◽  
Gradient Fractionation ◽  
And Function ◽  
Ig Synthesis

Normal human peripheral blood contains a population of T cells (autologous reactive cells [ARC]) capable of proliferating in response to signals from autologous B cells and monocytes. Selective suicide of proliferating ARC with 5-bromo-2-deoxyuridine and light demonstrated that this ARC was responsive to signals coded for by genes more closely linked to the HLA-DR, than to the HLA-A, or HLA-B, loci. Density-gradient fractionation of T cells indicated that populations enriched in ARC reactivity were also enriched for helper influences required for Ig synthesis by autologous B cells. In contrast, populations negatively selected for proliferating ARC were deficient in helper activity. These studies indicate that the ARC is responsive, at least in part, to products of genes closely linked to the HLA-DR locus and can function as a helper cell.

Circulating IL-17-producing mucosal-associated invariant T cells (MAIT) are associated with symptoms in children with asthma

2018 ◽  
Vol 188 ◽  
pp. 7-11 ◽  
Author(s):  
Guillaume Lezmi ◽  
Rola Abou Taam ◽  
Céline Dietrich ◽  
Lucienne Chatenoud ◽  
Jacques de Blic ◽  
...  
Keyword(s):  
T Cells ◽  
Children With Asthma ◽  
Invariant T Cells

scholarly journals Targeting of an activated T-cell subset using a bispecific antibody- toxin conjugate directed against CD4 and CD26

Blood ◽  
1993 ◽  
Vol 82 (7) ◽  
pp. 2224-2234
Author(s):  
JS Duke-Cohan ◽  
C Morimoto ◽  
SF Schlossman
Keyword(s):  
T Cells ◽  
T Cell ◽  
B Cell ◽  
Rational Design ◽  
Bispecific Antibody ◽  
Cell Subset ◽  
Activated T Cells ◽  
T Cell Subset ◽  
Tissue Grafts ◽  
Ig Synthesis

We have developed a bispecific antibody that recognizes the CD4 and CD26 antigens simultaneously and that was examined for its ability to target CD4+CD26+T cells. These latter cells constitute the activated component of the CD4+ CD29highCD45RO+ memory T-cell subset that provides help for B-cell Ig synthesis and help for responses against recall antigens. The purified bispecific antibody exhibited an estimated dissociation constant (kd) of 2.4 x 10(-9) mol/L, on comparison with 1.1 x 10(-9) mol/L for anti-CD26, and 1.6 x 10(-10) mol/L for anti-CD4. Surface plasmon resonance was used to show the bifunctional capacity of the antibody. On binding 125I-bispecific antibody to phytohemagglutinin (PHA)-activated T cells, 54.4% of the bound antibody was internalized. This was the result of bispecific binding, because monovalent fragments of anti-CD4 and anti-CD26 were not able to modulate antigen or induce internalization using both a fluorescent assay and an 125I-internalization assay. The ability of the bispecific antibody to be internalized was used to deliver a toxin, blocked ricin, specifically to cells that are CD4+CD26+. The inability of monovalent fragments to be internalized formed the basis for our hypothesis that monovalent binding by the bispecific immunotoxin would not result in internalization. Against resting E+ T cells, the bispecific immunotoxin developed a minimal effect. On preactivating the same cells, using phorbol myristate acetate (PMA)/ionomycin on concanavalin A (ConA) or especially PHA, levels of CD26 were upregulated and the immunotoxin effectively inhibited the ability to provide help for B-cell Ig synthesis while leaving intact the CD4-CD26+ and CD4+CD26- populations; an effect observed both functionally and by phenotype. The bispecific antibody proved to be most effective at inhibiting a heterologous mixed leukocyte reaction. We propose that this reagent may form the basis for the rational design of toxins designed to modulate activated T cells from, or directed against, tissue grafts.

scholarly journals Functional properties of neoplastic T cells in adult T cell lymphoma/leukemia patients from the Caribbean

Blood ◽  
1984 ◽  
Vol 63 (2) ◽  
pp. 477-481 ◽  
Author(s):  
F Miedema ◽  
FG Terpstra ◽  
JW Smit ◽  
S Daenen ◽  
W Gerrits ◽  
...  
Keyword(s):  
T Cells ◽  
T Cell ◽  
Functional Properties ◽  
Cell Lymphoma ◽  
T Cell Lymphoma ◽  
Pokeweed Mitogen ◽  
Neoplastic Cells ◽  
Human T Cell ◽  
The Caribbean ◽  
Ig Synthesis

Abstract The neoplastic T cells from five patients with adult T cell lymphoma/leukemia (ATLL), born in the Caribbean, were studied with respect to immunoregulatory activity on pokeweed mitogen (PWM) driven immunoglobulin (Ig) synthesis as well as surface-marker phenotypes with monoclonal antibodies. The neoplastic T cells in all patients had an OKT1+4+8–11+M1-I1–3A1- phenotype, but differed in the reactivity with OKT3. None of the patients' cells exerted helper activity on PWM- induced Ig synthesis. The neoplastic cells of three patients had suppressor activity on PWM-induced Ig synthesis. All patients were positive for human T cell leukemia/lymphoma virus (HTLV) or had antibodies against HTLV antigens. It has previously been shown that the neoplastic cells in Japanese ATLL patients and in patients from the Caribbean are indistinguishable by morphology and marker phenotype. We now show them to be also similar with respect to their functional properties.

scholarly journals Targeting of an activated T-cell subset using a bispecific antibody- toxin conjugate directed against CD4 and CD26

Blood ◽  
1993 ◽  
Vol 82 (7) ◽  
pp. 2224-2234 ◽  
Author(s):  
JS Duke-Cohan ◽  
C Morimoto ◽  
SF Schlossman
Keyword(s):  
T Cells ◽  
T Cell ◽  
B Cell ◽  
Rational Design ◽  
Bispecific Antibody ◽  
Cell Subset ◽  
Activated T Cells ◽  
T Cell Subset ◽  
Tissue Grafts ◽  
Ig Synthesis

Abstract We have developed a bispecific antibody that recognizes the CD4 and CD26 antigens simultaneously and that was examined for its ability to target CD4+CD26+T cells. These latter cells constitute the activated component of the CD4+ CD29highCD45RO+ memory T-cell subset that provides help for B-cell Ig synthesis and help for responses against recall antigens. The purified bispecific antibody exhibited an estimated dissociation constant (kd) of 2.4 x 10(-9) mol/L, on comparison with 1.1 x 10(-9) mol/L for anti-CD26, and 1.6 x 10(-10) mol/L for anti-CD4. Surface plasmon resonance was used to show the bifunctional capacity of the antibody. On binding 125I-bispecific antibody to phytohemagglutinin (PHA)-activated T cells, 54.4% of the bound antibody was internalized. This was the result of bispecific binding, because monovalent fragments of anti-CD4 and anti-CD26 were not able to modulate antigen or induce internalization using both a fluorescent assay and an 125I-internalization assay. The ability of the bispecific antibody to be internalized was used to deliver a toxin, blocked ricin, specifically to cells that are CD4+CD26+. The inability of monovalent fragments to be internalized formed the basis for our hypothesis that monovalent binding by the bispecific immunotoxin would not result in internalization. Against resting E+ T cells, the bispecific immunotoxin developed a minimal effect. On preactivating the same cells, using phorbol myristate acetate (PMA)/ionomycin on concanavalin A (ConA) or especially PHA, levels of CD26 were upregulated and the immunotoxin effectively inhibited the ability to provide help for B-cell Ig synthesis while leaving intact the CD4-CD26+ and CD4+CD26- populations; an effect observed both functionally and by phenotype. The bispecific antibody proved to be most effective at inhibiting a heterologous mixed leukocyte reaction. We propose that this reagent may form the basis for the rational design of toxins designed to modulate activated T cells from, or directed against, tissue grafts.

Sign in / Sign up

Export Citation Format

Share Document