Changes in sensitivity of peripheral lymphocytes of autoimmune gld mice to FasL-mediated apoptosis reveal a mechanism for the preferential deletion of CD4-CD8-B220+ T cells

Abstract Background Kawasaki disease is an autoimmune disease characterized by systemic vasculitis of unknown aetiology and most commonly occurs in children under 5 years old. Previous studies have found that the over-activation of lymphocytes is an important mechanism of Kawasaki disease. Activin A, also known as immunosuppressive factor P, is a multifunctional growth and transforming factor. However, whether activin A is involved in the regulation of peripheral lymphocytes activity in Kawasaki disease is unclear. Thus, we aimed to investigate the effect of activin A on the activity of peripheral lymphocytes in acute-phase Kawasaki disease. Methods Seven patients with Kawasaki disease and seven healthy controls were studied. Peripheral blood lymphocytes were isolated by Ficoll density gradient centrifugation. The activation of CD4+ and CD8+ T cells and CD19+ B cells was investigated by flow cytometry. The expression of activin type IIA receptors was investigated by flow cytometry. Results Immune imbalance in CD4 and CD8 lymphocytes were detected in acute-phase Kawasaki disease. The expression of activin type IIA receptors on CD8+ T cells and CD19+ B cells was increased in acute-phase Kawasaki disease and decreased following treatment with activin A. Activin A suppressed the expression of CD25 and CD69 on CD8+ T cells and the expression of CD69 on CD19+ B cells. Conclusions The expression of activin type IIA receptor was increased on CD8+ T cells and CD19+ B cells in Kawasaki disease. Activin A suppressed the expression of CD25, CD69 and activin type IIA receptors on peripheral CD8+ T lymphocyte. Activin A plays different roles in different lymphocyte subsets and suppresses peripheral CD8+ T lymphocyte activity in acute-phase Kawasaki disease.

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Differential Expression of the T cell Inhibitor TIGIT in Glioblastoma and Multiple Sclerosis

10.1101/591131 ◽

2019 ◽

Author(s):

Liliana E. Lucca ◽

Benjamin A. Lerner ◽

Danielle DeBartolo ◽

Calvin Park ◽

Gerald Ponath ◽

...

Keyword(s):

Multiple Sclerosis ◽

T Cells ◽

T Cell ◽

Differential Expression ◽

Peripheral Lymphocytes ◽

Immune Pathways ◽

Novel Targets ◽

The Brain

AbstractTo identify co-inhibitory immune pathways important in the brain, we hypothesized that comparison of T cells in lesions from patients with MS with tumor infiltrating T cells (TILs) from patients with GBM may reveal novel targets for immunotherapy. Focusing on PD-1 and TIGIT, we found that TIGIT and its ligand CD155 were highly expressed on GBM TILs but were near-absent in MS lesions, while lymphocytic expression of PD-1/PDL-1 was comparable. TIGIT was also upregulated in peripheral lymphocytes in GBM, suggesting recirculation of TILs. These data raise the possibility that anti-TIGIT therapy may be beneficial for patients with glioblastoma.

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B- AND T-CELLS AND INTRACELLULAR Ig-SYNTHESIS OF PERIPHERAL LYMPHOCYTES IN CHILDREN WITH ASTHMA AND/OR PREVIOUS ADENO-TONSILLECTOMY

Acta Pathologica Microbiologica Scandinavica Section C Immunology ◽

10.1111/j.1699-0463.1977.tb03668.x ◽

2009 ◽

Vol 85C (6) ◽

pp. 454-462

Author(s):

Poul Aabel ØStergaard

Keyword(s):

T Cells ◽

Peripheral Lymphocytes ◽

Children With Asthma ◽

Ig Synthesis

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Isolation of neoantigen-specific T cells from tumor and peripheral lymphocytes

Journal of Clinical Investigation ◽

10.1172/jci82416 ◽

2015 ◽

Vol 125 (10) ◽

pp. 3981-3991 ◽

Cited By ~ 200

Author(s):

Cyrille J. Cohen ◽

Jared J. Gartner ◽

Miryam Horovitz-Fried ◽

Katerina Shamalov ◽

Kasia Trebska-McGowan ◽

...

Keyword(s):

T Cells ◽

Peripheral Lymphocytes

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Ethyl Acetate Extract fromCelastrus aculeatusMerr. Suppresses Synovial Inflammation in Adjuvant Arthritis Rats through Apoptosis Induction of CD4+CD25+FOXP3+T Cells

Evidence-based Complementary and Alternative Medicine ◽

10.1155/2014/460136 ◽

2014 ◽

Vol 2014 ◽

pp. 1-8 ◽

Cited By ~ 2

Author(s):

Shu-tong Bai ◽

Pei-hong Chen ◽

Yu-yao Chen ◽

Xiao-chun Lin ◽

Jun-shan Liu ◽

...

Keyword(s):

T Cells ◽

Regulatory T Cells ◽

Ethyl Acetate ◽

Adjuvant Arthritis ◽

Ethyl Acetate Extract ◽

Joint Inflammation ◽

Suppressive Effect ◽

Synovial Inflammation ◽

Apoptosis Induction ◽

Peripheral Lymphocytes

Celastrus aculeatusMerr. has been widely used in traditional Chinese medicine to treat rheumatoid arthritis (RA) in clinic. However, the main active fraction of this plant is still unclear. In this study, we attempted to evaluate the suppressive effect of ethyl acetate extract (EAE) fromCelastrus aculeatusMerr. on synovial inflammation in adjuvant arthritis (AA) rats induced byMycobacterium tuberculosisH37Ra (Mtb) and to explore the underlying mechanisms. SD rats immunized with heat-killed Mtb were fed with EAE and observed for erythema, swelling, and induration of each paw. The pathologic changes in joint synovium were tested by hematoxylin-eosin staining. Apoptosis induction of synoviocytes was tested immunohistochemically. Apoptosis of peripheral lymphocytes and the level of regulatory T cells were analyzed by flow cytometry. After treatment with EAE, the joint inflammation in rats with AA was alleviated. Both apoptotic ratios of synoviocytes and peripheral lymphocytes and the ratio of CD4+CD25+FOXP3+to CD4 regulatory T cells were significantly increased. In summary, we first demonstrated that EAE ofCelastrus aculeatusMerr. can inhibit synovial inflammation in AA rats through apoptosis induction of CD4+CD25+FOXP3+T cells. Our study provides a rationale for the application ofCelastrus aculeatusMerr. to treat RA.

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