DIFFERENCES IN SEQUENCES OF RIBOSOMAL DNA SECOND INTERNAL TRANSCRIBED SPACER AMONG THREE MEMBERS OF ANOPHELES HYRCANUS COMPLEX FROM THE REPUBLIC OF KOREA

AbstractFor the first time, DNA sequence data were obtained for three species of Trichostrongylus from Russia. Internal transcribed spacer (ITS-2) of ribosomal DNA was sequenced for T. axei, T. colubriformis and T. probolurus from sheep from the Moscow region. ITS-2 rDNA length was estimated as 238 nucleotides for T. colubriformis and T. probolurus and 237 nucleotides for T. axei. The G+C content of the ITS-2 sequences of T. colubriformis, T. axei and T. probolurus were 31 %, 32 % and 34 % respectively. The level of interspecific differences in ITS-2 of rDNA of T. axei, T. probolurus and T. colubriformis ranged from 3 to 4 %. The ITS-2 sequences from the Russian specimens were compared with those of T. axei, T. probolurus and T. colubriformis from Australia and Germany. Intraspecific variation ranged from 0 % in T. colubriformis to 3.0 % in T. axei.

Download Full-text

Intraspecific Variation of Second Internal Transcribed Spacer of Nuclear Ribosomal DNA Among Populations of Anopheles (Kerteszia) cruzii (Diptera: Culicidae)

Journal of Medical Entomology ◽

10.1603/0022-2585(2007)44[538:ivosit]2.0.co;2 ◽

2007 ◽

Vol 44 (3) ◽

pp. 538-542 ◽

Cited By ~ 7

Author(s):

Rosely Dos S. Malafronte ◽

Mauro T. Marrelli ◽

Corália C. L. Ramirez ◽

Maíra N. Nassar ◽

Osvaldo Marinotti

Keyword(s):

Intraspecific Variation ◽

Ribosomal Dna ◽

Internal Transcribed Spacer ◽

Nuclear Ribosomal Dna ◽

Second Internal Transcribed Spacer

Download Full-text

Identification of Closely Related Hydrobaenus Species (Diptera: Chironomidae) Using the Second Internal Transcribed Spacer (ITS2) Region of Ribosomal DNA

Aquatic Insects ◽

10.1080/01650420412331327295 ◽

2004 ◽

Vol 26 (3-4) ◽

pp. 207-213 ◽

Cited By ~ 1

Author(s):

Hidetake Asari ◽

Shiro Kasuya ◽

Tadashi Kobayashi ◽

Shigeo Kondo ◽

Isao Nagano ◽

...

Keyword(s):

Ribosomal Dna ◽

Internal Transcribed Spacer ◽

Its2 Region ◽

Second Internal Transcribed Spacer

Download Full-text

A new subfamily for a clade of opecoelids (Trematoda: Digenea) exploiting marine fishes as second-intermediate hosts, with the first report of opecoelid metacercariae from an elasmobranch

Zoological Journal of the Linnean Society ◽

10.1093/zoolinnean/zlz084 ◽

2019 ◽

Author(s):

Storm Blas Martin ◽

Abigail Jayne Downie ◽

Thomas Herbert Cribb

Keyword(s):

Ribosomal Dna ◽

Internal Transcribed Spacer ◽

Marine Fishes ◽

Barrier Reef ◽

Intermediate Hosts ◽

Internal Transcribed Spacer Region ◽

Epaulette Shark ◽

The Family ◽

Unidentified Species ◽

Second Internal Transcribed Spacer

Abstract Metacercariae of trematodes belonging to the family Opecoelidae were collected from small fishes of the Great Barrier Reef: a blenniid, two gobiids, two labrids, three pomacentrids, a monacanthid, an ostraciid and the epaulette shark, Hemiscyllium ocellatum. Sequences of the second internal transcribed spacer region (ITS2) of ribosomal DNA were generated from these metacercariae in an attempt to match them with adult worms. Three species of Allopodocotyle (Allopodocotyle epinepheli, Allopodocotyle heronensis and an unidentified species), two unidentified species of Hamacreadium and Pacificreadium serrani were detected. Among the Opecoelidae, these species all resolve to a single, phylogenetically and somewhat morphologically distinct clade. Species of this clade are the only known marine opecoelids to exploit fishes as second-intermediate hosts. The clade is proposed to warrant a new subfamily, the Hamacreadiinae subfam. nov. It includes Allopodocotyle, Bentholebouria, Cainocreadium, Choanotrema, Hamacreadium, Pacificreadium, Paraplagioporus, Pedunculacetabulum and Podocotyloides.

Download Full-text