Lack of intraspecific variation in the second Internal Transcribed Spacer (ITS-2) of Trichostrongylus colubriformis ribosomal DNA

1993 ◽  
Vol 23 (8) ◽  
pp. 1069-1071 ◽  
Author(s):  
Hervé Hoste ◽  
Robin B. Gasser ◽  
Neil B. Chilton ◽  
Serge Mallet ◽  
Ian Beveridge
Keyword(s):  
Intraspecific Variation ◽  
Ribosomal Dna ◽  
Internal Transcribed Spacer ◽  
Trichostrongylus Colubriformis ◽  
Second Internal Transcribed Spacer

scholarly journals Sequences of the second internal transcribed spacer of ribosomal DNA for three species of Trichostrongylus (Nematoda: Trichostrongylidae) from sheep in Russia

2007 ◽  
Vol 44 (2) ◽  
pp. 43-46 ◽  
Author(s):  
D. Kuznetsov ◽  
N. Kuznetsova
Keyword(s):  
Dna Sequence ◽  
Intraspecific Variation ◽  
Ribosomal Dna ◽  
Internal Transcribed Spacer ◽  
Sequence Data ◽  
Moscow Region ◽  
Dna Sequence Data ◽  
Interspecific Differences ◽  
Second Internal Transcribed Spacer ◽  
First Time

AbstractFor the first time, DNA sequence data were obtained for three species of Trichostrongylus from Russia. Internal transcribed spacer (ITS-2) of ribosomal DNA was sequenced for T. axei, T. colubriformis and T. probolurus from sheep from the Moscow region. ITS-2 rDNA length was estimated as 238 nucleotides for T. colubriformis and T. probolurus and 237 nucleotides for T. axei. The G+C content of the ITS-2 sequences of T. colubriformis, T. axei and T. probolurus were 31 %, 32 % and 34 % respectively. The level of interspecific differences in ITS-2 of rDNA of T. axei, T. probolurus and T. colubriformis ranged from 3 to 4 %. The ITS-2 sequences from the Russian specimens were compared with those of T. axei, T. probolurus and T. colubriformis from Australia and Germany. Intraspecific variation ranged from 0 % in T. colubriformis to 3.0 % in T. axei.

scholarly journals Intragenomic variation in the second internal transcribed spacer of the ribosomal DNA of species of the genera Culex and Lutzia (Diptera: Culicidae)

2011 ◽  
Vol 106 (1) ◽  
pp. 01-08 ◽  
Author(s):  
Fabiana Tavares Vesgueiro ◽  
Bruna Demari-Silva ◽  
Rosely dos Santos Malafronte ◽  
Maria Anice Mureb Sallum ◽  
Mauro Toledo Marrelli
Keyword(s):  
Ribosomal Dna ◽  
Internal Transcribed Spacer ◽  
Intragenomic Variation ◽  
Second Internal Transcribed Spacer

scholarly journals Intraspecific variation in the first internal transcribed spacer (ITS1) of the nuclear ribosomal DNA inMelipona subnitida(Hymenoptera, Apidae), an endemic stingless bee from northeastern Brazil

Apidologie ◽  
2006 ◽  
Vol 37 (3) ◽  
pp. 376-386 ◽  
Author(s):  
Darci de Oliveira Cruz ◽  
Daniel Macedo de Melo Jorge ◽  
Júlio Otávio Portela Pereira ◽  
Davi Coe Torres ◽  
Carlos Eduardo Alves Soares ◽  
...  
Keyword(s):  
Intraspecific Variation ◽  
Ribosomal Dna ◽  
Internal Transcribed Spacer ◽  
Stingless Bee ◽  
Northeastern Brazil ◽  
Nuclear Ribosomal Dna

Minimal intraspecific variation in the sequence of the transcribed spacer regions of the ribosomal DNA of lake trout (Salvelinus namaycush)

Genome ◽  
1994 ◽  
Vol 37 (4) ◽  
pp. 664-671 ◽  
Author(s):  
Lang Zhuo ◽  
S. L. Sajdak ◽  
R. B. Phillips
Keyword(s):  
Polymerase Chain Reaction ◽  
Intraspecific Variation ◽  
Ribosomal Dna ◽  
Internal Transcribed Spacer ◽  
Lake Trout ◽  
Intergenic Spacer ◽  
Coding Region ◽  
Chain Reaction ◽  
Internal Transcribed Spacer Region ◽  
Polymerase Chain

Intraspecific variation in the sequence of the transcribed spacer regions of the ribosomal DNA (rDNA) in lake trout was examined by restriction mapping and sequencing of these regions amplified by the polymerase chain reaction. The length of the first internal transcribed spacer region (ITS-1) was 566 bases and the second internal transcribed spacer region (ITS-2) was 368 bases in lake trout. When the 1.4-kb region including the ITS-1, the 5.8S coding region, and the ITS-2 was amplified from 12 individuals from four populations and digested with eight different enzymes only one intraindividual polymorphism was found that occurred in each population. When the amplified ITS-1 region was sequenced from an additional 10 individuals from five populations, no interindividual variation was found in the sequence. A 6-kb portion of the rDNA repeat unit including 1.6 kb of the 18S coding region, the 5′ external spacer region (5′ ETS), and part of the adjacent intergenic spacer was cloned and a restriction map was prepared for these regions in lake trout. No intraspecific variation was found in the region adjacent to the 18S rDNA, which includes the 5′ ETS, although intraspecific and intraindividual length variation was found in the intergenic spacer region 3–6 kb from the 18S. Sequencing of a 609-b segment of the 5′ ETS adjacent to the 18S coding region revealed the presence of two 41-b repeats. The 198-b sequence between the repeats had some similarity to the 18S coding region of other fishes. Primers were designed for amplification of 559 b of the 5′ ETS using the polymerase chain reaction. No intraspecific variation in this region in lake trout was found when the DNA amplified from this region in 12 individuals from four populations was digested with eight restriction enzymes.Key words: ribosomal DNA, internal transcribed spacer regions, 5′ external spacer region, transcribed spacer, lake trout.

Identification of Closely Related Hydrobaenus Species (Diptera: Chironomidae) Using the Second Internal Transcribed Spacer (ITS2) Region of Ribosomal DNA

2004 ◽  
Vol 26 (3-4) ◽  
pp. 207-213 ◽  
Author(s):  
Hidetake Asari ◽  
Shiro Kasuya ◽  
Tadashi Kobayashi ◽  
Shigeo Kondo ◽  
Isao Nagano ◽  
...  
Keyword(s):  
Ribosomal Dna ◽  
Internal Transcribed Spacer ◽  
Its2 Region ◽  
Second Internal Transcribed Spacer

scholarly journals A new subfamily for a clade of opecoelids (Trematoda: Digenea) exploiting marine fishes as second-intermediate hosts, with the first report of opecoelid metacercariae from an elasmobranch

2019 ◽  
Author(s):  
Storm Blas Martin ◽  
Abigail Jayne Downie ◽  
Thomas Herbert Cribb
Keyword(s):  
Ribosomal Dna ◽  
Internal Transcribed Spacer ◽  
Marine Fishes ◽  
Barrier Reef ◽  
Intermediate Hosts ◽  
Internal Transcribed Spacer Region ◽  
Epaulette Shark ◽  
The Family ◽  
Unidentified Species ◽  
Second Internal Transcribed Spacer

Abstract Metacercariae of trematodes belonging to the family Opecoelidae were collected from small fishes of the Great Barrier Reef: a blenniid, two gobiids, two labrids, three pomacentrids, a monacanthid, an ostraciid and the epaulette shark, Hemiscyllium ocellatum. Sequences of the second internal transcribed spacer region (ITS2) of ribosomal DNA were generated from these metacercariae in an attempt to match them with adult worms. Three species of Allopodocotyle (Allopodocotyle epinepheli, Allopodocotyle heronensis and an unidentified species), two unidentified species of Hamacreadium and Pacificreadium serrani were detected. Among the Opecoelidae, these species all resolve to a single, phylogenetically and somewhat morphologically distinct clade. Species of this clade are the only known marine opecoelids to exploit fishes as second-intermediate hosts. The clade is proposed to warrant a new subfamily, the Hamacreadiinae subfam. nov. It includes Allopodocotyle, Bentholebouria, Cainocreadium, Choanotrema, Hamacreadium, Pacificreadium, Paraplagioporus, Pedunculacetabulum and Podocotyloides.

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