Molecular Breeding of Polysaccharide-Utilizing Yeast Cells by Cell Surface Engineering

AbstractHalloysite clay nanotubes are safe and biocompatible nanomaterials and their application in biomaterials is very promising. The microencapsulation of yeast cells in the shell of clay nanotubes modifying their properties was demonstrated here. Each cell was coated with a 200–300 nm-thick tube shell and this coating was not harmful for these cells’ reproduction. Synthesis of magnetic nanoparticles on the surfaces of the nanotubes allowed for magnetic-field manipulation of the coated cells, including their separation. Providing nano-designed shells for biological cells is a step forward in development of ‘cyborg’ microorganisms combining their intrinsic properties with functions added through nano-engineering.

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Patterning of Growth Factor Gradients in Embryoid Bodies via Extracellular Matrix-Assisted Glycocalyx Engineering

10.26434/chemrxiv.12755849.v1 ◽

2020 ◽

Author(s):

Matthew R. Naticchia ◽

Logan K. Laubach ◽

honigfort Daniel J. ◽

purcell Sean C. ◽

Kamil Godula

Keyword(s):

Stem Cells ◽

Extracellular Matrix ◽

Growth Factor ◽

Cell Surface ◽

Surface Engineering ◽

Embryoid Bodies ◽

Cell Surface Engineering

Cell surface engineering with synthetic glycomimetic co-receptors for FGF2 was used to establish gradients of stem cells with enhanced FGF2 affinity in embryoid bodies (EBs). Gradient shape was controlled by pre-assembly of glycomimetics into nanoscale vesicles with tunable dimensions and EB penetrance. <br>

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Cell Surface Engineering of a β-Galactosidase for Galactooligosaccharide Synthesis

Applied and Environmental Microbiology ◽

10.1128/aem.00326-09 ◽

2009 ◽

Vol 75 (18) ◽

pp. 5938-5942 ◽

Cited By ~ 15

Author(s):

Yumei Li ◽

Lili Lu ◽

Hongmei Wang ◽

Xiaodong Xu ◽

Min Xiao

Keyword(s):

Cell Surface ◽

Surface Engineering ◽

Yeast Cells ◽

Penicillium Expansum ◽

Yeast Nitrogen Base ◽

Gene Encoding ◽

Nitrogen Base ◽

Reading Frame ◽

Acid Protein ◽

Casamino Acids

ABSTRACT A novel gene encoding transglycosylating β-galactosidase (BGase) was cloned from Penicillium expansum F3. The sequence contained a 3,036-bp open reading frame encoding a 1,011-amino-acid protein. This gene was subsequently expressed on the cell surface of Saccharomyces cerevisiae EBY-100 by galactose induction. The BGase-anchored yeast could directly utilize lactose to produce galactooligosaccharide (GOS), as well as the by-products glucose and a small quantity of galactose. The glucose was consumed by the yeast, and the galactose was used for BGase expression, thus greatly facilitating GOS synthesis. The GOS yield reached 43.64% when the recombinant yeast was cultivated in yeast nitrogen base-Casamino Acids medium containing 100 g/liter initial lactose at 25°C for 5 days. The yeast cells were harvested and recycled for the next batch of GOS synthesis. During sequential operations, both oligosaccharide synthesis and BGase expression were maintained at high levels with GOS yields of over 40%, and approximately 8 U/ml of BGase was detected in each batch.

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