Effect of Shelter, Broodstock Number, and Sex-Ratio on Juvenile Production in the Swordtail Xiphophorus helleri under Intensive Culture Conditions

1998 ◽  
Vol 29 (1) ◽  
pp. 92-96 ◽  
Author(s):  
C. L. W. Jones ◽  
H. Kaiser ◽  
T Hecht
2017 ◽  
Vol 8 (1) ◽  
pp. 249-254 ◽  
Author(s):  
Paige A.C. Maskill ◽  
Isaac R. Miller ◽  
Leif J. Halvorson ◽  
Hilary B. Treanor ◽  
Cal W. Fraser ◽  
...  

Abstract Woundfin Plagopterus argentissimus are a small, endangered cyprinid found solely in the Virgin River across Utah, Arizona, and Nevada. As of now, only three facilities culture Woundfin for conservation propagation purposes, but there is growing concern that current extensive culture protocols are not meeting the conservation recovery goals outlined in the Virgin River Resource Management and Recovery Program. The development of alternative rearing methodology, like effective intensive culture conditions, could help increase production to more effectively meet the conservation propagation needs for this species. Our goal was to assess the effect of different sex ratios and fish densities on reproductive success at indoor facilities. We measured fertilization success at three different sex ratios (1:1, 1:3, 1:5; female:male) and three different fish densities (0.05, 0.21, and 0.47 fish/L) in a laboratory setting. Sex ratio did not significantly affect fertilization success (P = 0.73). Fertilization was completely inhibited at a density of 0.05 fish/L, but there was no difference in fertilization success between the two other density treatments (P = 0.92). Our findings suggest that a density greater than 0.05 fish/L and a sex ratio of 1:1, 1:3, or 1:5 will likely enhance fertilization success. The results and conclusions of this paper provide valuable rearing information for the Virgin River Resource Management and Recovery Program and hatchery managers attempting to increase production of Woundfin in an intensive culture setting.


2004 ◽  
Vol 66 (2) ◽  
pp. 239-245 ◽  
Author(s):  
Christian E. W. Steinberg ◽  
Thomas Meinelt ◽  
Kurt Schreckenbach ◽  
Klaus Knopf ◽  
Andreas Wienke ◽  
...  

Author(s):  
R. Rodríguez ◽  
J. D. Celada ◽  
M. Sáez-Royuela ◽  
J. M. Carral ◽  
A. Aguilera ◽  
...  

2019 ◽  
Vol 31 (1) ◽  
pp. 198
Author(s):  
F. Kotarski ◽  
B. Zimmer ◽  
C. Wrenzycki

The sex ratio of newborn calves and embryos produced in vivo is ~1:1. However, numerous studies on bovine in vitro-produced embryos suggest that the sex ratio may differ from 1:1 and that the rate of development may be influenced by the sex of the embryo under certain culture conditions. The duration of sperm-oocyte interaction and sperm pre-incubation also affect the sex ratio of bovine embryos produced in vitro. It is well documented that in vitro male embryos reach the more advanced stages earlier than do their female counterparts. Selection of developmentally more advanced embryos in anticipation that they have a greater developmental capacity may be one of the underlying causes of the disproportionate number of males among offspring born after transfer of in vitro-produced embryos. The aim of the present study is to test whether a pre-incubation of sperm before IVF might improve the developmental rates and also influence the sex ratio of the resulting embryos. Bovine cumulus-oocyte complexes were recovered from abattoir-derived ovaries by the slicing method. After 24h of maturation, fertilization was realised using a standard protocol. Prior to IVF, sperm cells from 2 different bulls were treated as follows: sperm within group A were pre-incubated in IVF medium for one hour. This step was omitted for sperm in group B (control). After 19h of co-culture of COC and sperm, presumptive zygotes were cultured in SOFaa for a period of 7 days. Cleavage and developmental rates were recorded at Day 3 and 7 (Day 0=IVF). Day 7 blastocysts from all groups were sexed using bovine and Y chromosome-specific primers. Data were analysed by ANOVA. As shown in Table 1, sperm pre-incubation did not affect the cleavage and developmental rates for the individual bull (P>0.05). On average, at Day 7 of development a higher number of blastocysts was determined when embryos had been produced from pre-incubated sperm (P ≤ 0.05). This held true for both bulls. The shift in favour of male embryos was detectable in all groups of embryos, with a drastic one for bull 1 after sperm pre-incubation. In conclusion, sperm pre-incubation accelerated embryo development and possibly enhanced the proportion of male embryos, which was already shifted toward males. Table 1.Developmental rates, developmental kinetics and sex ratio of embryos after sperm pre-incubation before IVF (mean±standard deviation)


1972 ◽  
Vol 34 (2) ◽  
pp. 100-102 ◽  
Author(s):  
Robert R. Stickney ◽  
Takeshi Murai ◽  
George O. Gibbons

1999 ◽  
Vol 15 (4-5) ◽  
pp. 345-345
Author(s):  
A. Sanz ◽  
M. García-Gallego ◽  
A. Domezain ◽  
M. Higuera

Sign in / Sign up

Export Citation Format

Share Document