scholarly journals Deletion of the signalling molecule synthase ScbA has pleiotropic effects on secondary metabolite biosynthesis, morphological differentiation and primary metabolism in Streptomyces coelicolor A3(2)

2010 ◽  
Vol 4 (2) ◽  
pp. 239-251 ◽  
Author(s):  
Davide D'Alia ◽  
Daniela Eggle ◽  
Kay Nieselt ◽  
Wei-Shou Hu ◽  
Rainer Breitling ◽  
...  
Keyword(s):  
Secondary Metabolite ◽  
Streptomyces Coelicolor ◽  
Morphological Differentiation ◽  
Pleiotropic Effects ◽  
Primary Metabolism ◽  
Signalling Molecule ◽  
Secondary Metabolite Biosynthesis

scholarly journals Mycelium Differentiation and Antibiotic Production in Submerged Cultures of Streptomyces coelicolor

2008 ◽  
Vol 74 (12) ◽  
pp. 3877-3886 ◽  
Author(s):  
Angel Manteca ◽  
Ruben Alvarez ◽  
Nuria Salazar ◽  
Paula Yagüe ◽  
Jesus Sanchez
Keyword(s):  
Secondary Metabolite ◽  
Antibiotic Production ◽  
Streptomyces Coelicolor ◽  
Growth Arrest ◽  
Morphological Differentiation ◽  
Developmental Model ◽  
Secondary Metabolite Production ◽  
Submerged Cultures ◽  
Metabolite Production ◽  
Developmental Features

ABSTRACT Despite the fact that most industrial processes for secondary metabolite production are performed with submerged cultures, a reliable developmental model for Streptomyces under these culture conditions is lacking. With the exception of a few species which sporulate under these conditions, it is assumed that no morphological differentiation processes take place. In this work, we describe new developmental features of Streptomyces coelicolor A3(2) grown in liquid cultures and integrate them into a developmental model analogous to the one previously described for surface cultures. Spores germinate as a compartmentalized mycelium (first mycelium). These young compartmentalized hyphae start to form pellets which grow in a radial pattern. Death processes take place in the center of the pellets, followed by growth arrest. A new multinucleated mycelium with sporadic septa (second mycelium) develops inside the pellets and along the periphery, giving rise to a second growth phase. Undecylprodigiosin and actinorhodin antibiotics are produced by this second mycelium but not by the first one. Cell density dictates how the culture will behave in terms of differentiation processes and antibiotic production. When diluted inocula are used, the growth arrest phase, emergence of a second mycelium, and antibiotic production are delayed. Moreover, pellets are less abundant and have larger diameters than in dense cultures. This work is the first to report on the relationship between differentiation processes and secondary metabolite production in submerged Streptomyces cultures.

Regio- and stereoselective intermolecular phenol coupling enzymes in secondary metabolite biosynthesis

2021 ◽  
Author(s):  
Wolfgang Hüttel ◽  
Michael Müller
Keyword(s):  
Secondary Metabolites ◽  
Secondary Metabolite ◽  
Metabolic Pathways ◽  
Cyp Enzymes ◽  
Secondary Metabolite Biosynthesis

Phenol coupling enzymes, especially laccases and CYP-enzymes create an enormous diversity of biarylic secondary metabolites in fungi, plants, and bacteria. The enzymes and the elucidation of the corresponding metabolic pathways are presented.

scholarly journals Transcriptional Profiles of Secondary Metabolite Biosynthesis Genes and Cytochromes in the Leaves of Four Papaver Species

Data ◽  
2018 ◽  
Vol 3 (4) ◽  
pp. 55 ◽  
Author(s):  
Dowan Kim ◽  
Myunghee Jung ◽  
In Ha ◽  
Min Lee ◽  
Seok-Geun Lee ◽  
...  
Keyword(s):  
Secondary Metabolite ◽  
Developmental Stages ◽  
Expression Profiles ◽  
Bioinformatics Analyses ◽  
Kegg Pathways ◽  
Benzylisoquinoline Alkaloid ◽  
Development Life Cycle ◽  
The Family ◽  
Leaf Transcriptome ◽  
Secondary Metabolite Biosynthesis

Poppies are well-known plants in the family Papaveraceae that are rich in alkaloids. This family contains 61 species, and in this study we sequenced the transcriptomes of four species’ (Papaver rhoeas, Papaver nudicaule, Papaver fauriei, and Papaver somniferum) leaves. These transcripts were systematically assessed for the expression of secondary metabolite biosynthesis (SMB) genes and cytochromes, and their expression profiles were assessed for use in bioinformatics analyses. This study contributed 265 Gb (13 libraries with three biological replicates) of leaf transcriptome data from three Papaver plant developmental stages. Sequenced transcripts were assembled into 815 Mb of contigs, including 226 Mb of full-length transcripts. The transcripts for 53 KEGG pathways, 55 cytochrome superfamilies, and benzylisoquinoline alkaloid biosynthesis (BIA) were identified and compared to four other alkaloid-rich genomes. Additionally, 22 different alkaloids and their relative expression profiles in three developmental stages of Papaver species were assessed by targeted metabolomics using LC-QTOF-MS/MS. Collectively, the results are given in co-occurrence heat-maps to help researchers obtain an overview of the transcripts and their differential expression in the Papaver development life cycle, particularly in leaves. Moreover, this dataset will be a valuable resource to derive hypotheses to mitigate an array of Papaver developmental and secondary metabolite biosynthesis issues in the future.

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