Added diagnostic value of broad‐range 16SPCRon periprosthetic tissue and clinical specimens from other normally sterile body sites

Diagnostic value of PCR for detection of borrelia burgdorferi DNA in clinical specimens from patients with erythema migrans and lyme neuroborreliosis

Molecular Diagnosis ◽

10.1054/xd.2000.7179 ◽

2000 ◽

Vol 5 (2) ◽

pp. 139-150 ◽

Cited By ~ 9

Author(s):

A LEBECH ◽

K HANSEN ◽

F BRANDRUP ◽

O CLEMMENSEN ◽

L HALKIERSORENSEN

Keyword(s):

Borrelia Burgdorferi ◽

Erythema Migrans ◽

Diagnostic Value ◽

Lyme Neuroborreliosis ◽

Clinical Specimens

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Diagnostic value of human cytomegalovirus DNA PCR regarding different clinical specimens

Cellular and Molecular Life Sciences ◽

10.1007/bf01919521 ◽

1996 ◽

Vol 52 (4) ◽

pp. 304-305

Author(s):

S. Prösch ◽

H. Meisel ◽

E. Schielke ◽

K. M. Einhäupl ◽

D. H. Krüger

Keyword(s):

Human Cytomegalovirus ◽

Diagnostic Value ◽

Clinical Specimens ◽

Dna Pcr

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Diagnostic Value of PCR for Detection of Borrelia burgdorferi DNA in Clinical Specimens From Patients With Erythema Migrans and Lyme Neuroborreliosis

Molecular Diagnosis ◽

10.2165/00066982-200005020-00007 ◽

2000 ◽

Vol 5 (2) ◽

pp. 139-150 ◽

Cited By ~ 63

Author(s):

ANNE-METTE LEBECH ◽

KLAUS HANSEN ◽

FLEMMING BRANDRUP ◽

OLE CLEMMENSEN ◽

LARS HALKIER-SØRENSEN

Keyword(s):

Borrelia Burgdorferi ◽

Erythema Migrans ◽

Diagnostic Value ◽

Lyme Neuroborreliosis ◽

Clinical Specimens

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Diagnostic Value of Electron Microscopy in Soft Tissue Tumors

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100068096 ◽

1970 ◽

Vol 28 ◽

pp. 220-221

Author(s):

Gerald Fine ◽

Azorides R. Morales

Keyword(s):

Cardiac Myxoma ◽

Osteogenic Sarcoma ◽

Diagnostic Value ◽

Soft Tissue Tumors ◽

Amelanotic Melanoma ◽

Growth Patterns ◽

Light Microscopic Level ◽

Mesenchymal Tumors ◽

Good Agreement

For years the separation of carcinoma and sarcoma and the subclassification of sarcomas has been based on the appearance of the tumor cells and their microscopic growth pattern and information derived from certain histochemical and special stains. Although this method of study has produced good agreement among pathologists in the separation of carcinoma from sarcoma, it has given less uniform results in the subclassification of sarcomas. There remain examples of neoplasms of different histogenesis, the classification of which is questionable because of similar cytologic and growth patterns at the light microscopic level; i.e. amelanotic melanoma versus carcinoma and occasionally sarcoma, sarcomas with an epithelial pattern of growth simulating carcinoma, histologically similar mesenchymal tumors of different histogenesis (histiocytoma versus rhabdomyosarcoma, lytic osteogenic sarcoma versus rhabdomyosarcoma), and myxomatous mesenchymal tumors of diverse histogenesis (myxoid rhabdo and liposarcomas, cardiac myxoma, myxoid neurofibroma, etc.)

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A combined pseudoreplica-immunocytochemical technique for research and diagnostic virology

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100162065 ◽

1990 ◽

Vol 48 (3) ◽

pp. 900-901

Author(s):

Blayne Fritz ◽

Stanley J. Naides ◽

Kenneth Moore

Keyword(s):

Phosphotungstic Acid ◽

Immunogold Labelling ◽

Uranyl Acetate ◽

Distilled Water ◽

Clinical Specimens ◽

Tissue Sections ◽

Specimen Retrieval ◽

Transmission Electron ◽

Viral Particles ◽

Diagnostic Virology

The pseudoreplica method of staining viral particles for visualization by transmission electron microscopy is a very popular technique. The ability to concentrate clinical specimens while semi-embedding viral particles makes it especially well suited for morphologic and diagnostic virology. Immunolabelling viral particles with colloidal gold is a technique frequently employed by both research and diagnostic virologists. We have characterized a procedure which provides the advantage of both by modifying and combining pseudoreplica staining and immunogold labelling.Modification of specimen retrieval and delay of staining allows us to utilize pseudoreplica processed specimens within our standard immunogold labelling protocol. In brief, we absorbed samples onto 2% agarose, added.25% Formvar and wicked dry. We then floated the Formvar-virus film onto double distilled water, added grids and retrieved with parafilm. The Formvar-virus specimens were then treated as thin tissue sections within our standard two stage immunolabelling protocol. Following completion of immunogold labelling; each grid was negatively stained with phosphotungstic acid or uranyl acetate contrast stains.

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