Immunogenicity of recombinant outer membrane porin protein and protective efficacy against lethal challenge with Bordetella bronchiseptica in rabbits

2019 ◽  
Vol 127 (6) ◽  
pp. 1646-1655 ◽  
Author(s):  
H. Zhang ◽  
H. Zhang ◽  
B. Xiong ◽  
G. Fan ◽  
Z. Cao
Keyword(s):  
Outer Membrane ◽  
Protective Efficacy ◽  
Bordetella Bronchiseptica ◽  
Lethal Challenge ◽  
Porin Protein ◽  
Outer Membrane Porin

scholarly journals Sialylation of Outer Membrane Porin Protein D: A Mechanistic Basis of Antibiotic Uptake inPseudomonas aeruginosa

2014 ◽  
Vol 13 (6) ◽  
pp. 1412-1428 ◽  
Author(s):  
Biswajit Khatua ◽  
Jeremy Van Vleet ◽  
Biswa Pronab Choudhury ◽  
Rama Chaudhry ◽  
Chitra Mandal
Keyword(s):  
Outer Membrane ◽  
Porin Protein ◽  
Outer Membrane Porin ◽  
Mechanistic Basis

Immunobiology of purified recombinant outer membrane porin protein I of Neisseria gonorrhoeae

1994 ◽  
Vol 14 (5) ◽  
pp. 1059-1075 ◽  
Author(s):  
Christopher Elkins ◽  
Katherine B. Barkley ◽  
Nicholas H. Carbonetti ◽  
Alex J. Coimbre ◽  
P. Frederick Sparling
Keyword(s):  
Neisseria Gonorrhoeae ◽  
Outer Membrane ◽  
Porin Protein ◽  
Outer Membrane Porin

scholarly journals Protection Elicited by Native Outer Membrane Protein Oms66 (p66) against Host-Adapted Borrelia burgdorferi: Conformational Nature of Bactericidal Epitopes

2000 ◽  
Vol 68 (5) ◽  
pp. 2647-2654 ◽  
Author(s):  
Maurice M. Exner ◽  
Xiaoyang Wu ◽  
David R. Blanco ◽  
James N. Miller ◽  
Michael A. Lovett
Keyword(s):  
Borrelia Burgdorferi ◽  
Outer Membrane ◽  
Bactericidal Activity ◽  
High Titer ◽  
Membrane System ◽  
Killing Activity ◽  
Porin Protein ◽  
Outer Membrane Porin ◽  
New Zealand White Rabbits ◽  
Mouse Ear

ABSTRACT Oms66 is a Borrelia burgdorferi outer membrane porin protein whose role in Lyme disease pathogenesis and immunity has not been well established. Oms66 was solubilized from whole-cell lysates of strain B313 (which is derived from B31 but lacks OspA, -B, -C, and -D) and purified to homogeneity by fast-protein liquid chromatography. Purified native Oms66 (nOms66), which retained the ability to form large channels in a planar lipid bilayer model membrane system, and denatured Oms66 (hOms66) were used to immunize New Zealand White rabbits. The resulting Oms66 antisera were tested in a complement-dependent borreliacidal assay in parallel with basal serum and with serum from rabbits immune to reinfection with B. burgdorferi (IRS). IRS showed high-titer complement-dependent killing of both strains B31 and B313. Sera from animals immunized with nOms66 showed high-titer complement-dependent killing activity against strain B313 but exhibited no killing of B31. By comparison, serum generated from immunizations with hOms66 showed no killing activity against either strain. Following adsorption of antiserum to nOms66 with recombinant Oms66 (rOms66), the serum antibodies no longer bound to rOms66 or to nOms66 that had been denatured with 8 M urea. However, the antibodies still bound to nOms66 and killing activity against B313 was retained, thus suggesting that native, conformational epitopes are targets of this bactericidal activity. Six C3H HeJ mice were immunized with nOms66 and were challenged using “host-adapted” B. burgdorferi B31 by skin implantation of infected mouse ear tissue. Four of the six mice were protected against both localized and disseminated infection. These findings indicate that native Oms66 can elicit potent bactericidal activity and significant protective immunity against host-adapted organisms.

scholarly journals Outer membrane porin protein ofCampylobacter jejuni

1986 ◽  
Vol 37 (3) ◽  
pp. 247-250 ◽  
Author(s):  
M. Huyer ◽  
T.R. Parr ◽  
R.E.W. Hancock ◽  
W.J. Page
Keyword(s):  
Outer Membrane ◽  
Porin Protein ◽  
Outer Membrane Porin ◽  
Ofcampylobacter Jejuni

scholarly journals Cloning of the Pseudomonas aeruginosa outer membrane porin protein P gene: evidence for a linked region of DNA homology.

1988 ◽  
Vol 170 (5) ◽  
pp. 2312-2318 ◽  
Author(s):  
R J Siehnel ◽  
E A Worobec ◽  
R E Hancock
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Outer Membrane ◽  
Dna Homology ◽  
P Gene ◽  
Porin Protein ◽  
Outer Membrane Porin

scholarly journals Each mammalian mitochondrial outer membrane porin protein is dispensable: effects on cellular respiration

1999 ◽  
Vol 1452 (1) ◽  
pp. 68-78 ◽  
Author(s):  
Shaolong Wu ◽  
Margaret J. Sampson ◽  
William K. Decker ◽  
William J. Craigen
Keyword(s):  
Outer Membrane ◽  
Cellular Respiration ◽  
Mitochondrial Outer Membrane ◽  
Porin Protein ◽  
Outer Membrane Porin

Channel forming outer membrane porin protein in halophile: Expressed as a soluble form in Escherichia coli

2013 ◽  
Vol 54 ◽  
pp. 44-50 ◽  
Author(s):  
Hiroko Tokunaga ◽  
Masafumi Furukawa ◽  
Tsutomu Arakawa ◽  
Masao Tokunaga
Keyword(s):  
Escherichia Coli ◽  
Outer Membrane ◽  
Soluble Form ◽  
Porin Protein ◽  
Outer Membrane Porin

scholarly journals Structure of the lc and nmpC outer membrane porin protein genes of lambdoid bacteriophage.

1986 ◽  
Vol 261 (27) ◽  
pp. 12723-12732
Author(s):  
A J Blasband ◽  
W R Marcotte ◽  
C A Schnaitman
Keyword(s):  
Outer Membrane ◽  
Porin Protein ◽  
Outer Membrane Porin

scholarly journals Stabilising and destabilising modifications of cysteines in the E. coli outer membrane porin protein OmpC

FEBS Letters ◽  
1997 ◽  
Vol 411 (2-3) ◽  
pp. 201-205 ◽  
Author(s):  
Isa Gokce ◽  
Graeme Bainbridge ◽  
Jeremy H Lakey
Keyword(s):  
Outer Membrane ◽  
E Coli ◽  
Porin Protein ◽  
Outer Membrane Porin
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