scholarly journals Rapid detection and quantification of plasmid‐mediated colistin resistance genes ( mcr‐1 to mcr‐5 ) by real‐time PCR in bacterial and environmental samples

2020 ◽  
Vol 129 (6) ◽  
pp. 1523-1529
Author(s):  
R. Tolosi ◽  
I. Apostolakos ◽  
A. Laconi ◽  
L. Carraro ◽  
G. Grilli ◽  
...  
Keyword(s):  
Real Time ◽  
Resistance Genes ◽  
Real Time Pcr ◽  
Rapid Detection ◽  
Environmental Samples ◽  
Colistin Resistance ◽  
Detection And Quantification

scholarly journals Development of a Multiplex Real-Time PCR Assay for Rapid Detection of Tigecycline Resistance Gene tet(X) Variants from Bacterial, Fecal, and Environmental Samples

2020 ◽  
Vol 64 (4) ◽  
Author(s):  
Yulin Fu ◽  
Dejun Liu ◽  
Huangwei Song ◽  
Zhihai Liu ◽  
Haiyang Jiang ◽  
...  
Keyword(s):  
Detection Limit ◽  
Real Time ◽  
Resistance Gene ◽  
Resistance Genes ◽  
Real Time Pcr ◽  
Rapid Detection ◽  
Environmental Samples ◽  
Target Gene ◽  
Pcr Assay ◽  
High Level

ABSTRACT We developed a multiplex real-time SYBR green-based PCR assay for rapid detection of tet(X) and its variants, including tet(X1) and tet(X2) and high-level tigecycline resistance genes tet(X3), tet(X4), and tet(X5). We showed that the real-time PCR assay developed had high linearity (R2 ≥ 0.996), sensitivity (low detection limit), and specificity (only the target gene could be amplified significantly) and further evaluated it using bacterial, fecal, and environmental samples.

scholarly journals Detection of Peanut Allergen Traces with a Real Time PCR Assay - The Challenge to Protect Food-Allergic Consumers

2017 ◽  
Vol 7 (1) ◽  
pp. 32 ◽  
Author(s):  
Dimitra Houhoula ◽  
Stamatios Koussissis ◽  
Vladimiros Lougovois ◽  
John Tsaknis ◽  
Dimitra Kassavita ◽  
...  
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Rapid Detection ◽  
Food Products ◽  
Molecular Techniques ◽  
Food Allergens ◽  
Pcr Assay ◽  
Peanut Allergen ◽  
Pcr Method ◽  
Detection And Quantification

The aim of the present study was the implementation of molecular techniques in the detection and quantification of allergic substances of peanut in various kinds of food products, e.g., breakfast cereals, chocolates and biscuits that are frequently related to allergies. In some cases, the presence of peanuts can be due to contamination during production and are not declared on the label. A total of 152 samples were collected from supermarkets and were analysed by a Real Time PCR method. The results indicated that 125 samples (83,3%) were found positive in peanut traces but the most important finding is that from the 84 samples that had no allergen declaration for peanuts, 48 (57,1%) of them were found positive. In conclusion, Real Time PCR can be a very important tool for the rapid detection and quantification of food allergens.

Real-time PCR assay for rapid detection and quantification of Campylobacter jejuni on chicken rinses from poultry processing plant

2007 ◽  
Vol 21 (3) ◽  
pp. 177-181 ◽  
Author(s):  
Aradom Debretsion ◽  
Tsegaye Habtemariam ◽  
Saul Wilson ◽  
David Nganwa ◽  
Teshome Yehualaeshet
Keyword(s):  
Real Time ◽  
Campylobacter Jejuni ◽  
Real Time Pcr ◽  
Rapid Detection ◽  
Processing Plant ◽  
Pcr Assay ◽  
Poultry Processing ◽  
Detection And Quantification
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