Antibody Detection and Molecular Characterization of Toxoplasma gondii from Bobcats (Lynx rufus ), Domestic Cats (Felis catus ), and Wildlife from Minnesota, USA

2016 ◽  
Vol 63 (5) ◽  
pp. 567-571 ◽  
Author(s):  
Shiv K. Verma ◽  
Larissa Minicucci ◽  
Darby Murphy ◽  
Michelle Carstensen ◽  
Carolin Humpal ◽  
...  
Keyword(s):  
Toxoplasma Gondii ◽  
Molecular Characterization ◽  
Felis Catus ◽  
Lynx Rufus ◽  
Antibody Detection ◽  
Domestic Cats

Isolation and Genetic Characterization of Toxoplasma gondii from Black Bears (Ursus americanus ), Bobcats (Lynx rufus ), and Feral Cats (Felis catus ) from Pennsylvania

2015 ◽  
Vol 62 (3) ◽  
pp. 410-415 ◽  
Author(s):  
Jitender P. Dubey ◽  
Shiv K. Verma ◽  
Rafael Calero-Bernal ◽  
Ana B. Cassinelli ◽  
Oliver C. H. Kwok ◽  
...  
Keyword(s):  
Toxoplasma Gondii ◽  
Ursus Americanus ◽  
Genetic Characterization ◽  
Felis Catus ◽  
Lynx Rufus ◽  
Black Bears ◽  
Feral Cats

Molecular characterization of Toxoplasma gondii in pork meat from different production systems in the Czech Republic

2016 ◽  
Vol 238 ◽  
pp. 252-255 ◽  
Author(s):  
Michal Slany ◽  
Nikol Reslova ◽  
Vladimir Babak ◽  
Alena Lorencova
Keyword(s):  
Czech Republic ◽  
Toxoplasma Gondii ◽  
Molecular Characterization ◽  
Production Systems ◽  
Pork Meat ◽  
The Czech Republic

First isolation and molecular characterization of Toxoplasma gondii from a human placenta in Argentina

2014 ◽  
Vol 63 (2) ◽  
pp. 470-472 ◽  
Author(s):  
Lais Pardini ◽  
Liliana A. Carral ◽  
Mariana Bernstein ◽  
María L. Gos ◽  
Patricia Olejnik ◽  
...  
Keyword(s):  
Toxoplasma Gondii ◽  
Molecular Characterization ◽  
Human Placenta

scholarly journals Coinfection by Toxoplasma gondii and Leishmania spp. in domestic cats (Felis catus) in State of Mato Grosso do Sul

2014 ◽  
Vol 47 (6) ◽  
pp. 796-797 ◽  
Author(s):  
Audrey Rennó Campos Braga ◽  
Ana Paula Ferreira Lopes Corrêa ◽  
Lucilene Granuzzio Camossi ◽  
Rodrigo Costa da Silva ◽  
Helio Langoni ◽  
...  
Keyword(s):  
Toxoplasma Gondii ◽  
Felis Catus ◽  
Domestic Cats ◽  
Mato Grosso ◽  
Leishmania Spp ◽  
Mato Grosso Do Sul

scholarly journals Prevalence ofToxoplasma gondiiand Other Gastrointestinal Parasites in Domestic Cats from Households in Thika Region, Kenya

2017 ◽  
Vol 2017 ◽  
pp. 1-6 ◽  
Author(s):  
Adele Nyambura Njuguna ◽  
John Maina Kagira ◽  
Simon Muturi Karanja ◽  
Maina Ngotho ◽  
Lucy Mutharia ◽  
...  
Keyword(s):  
Toxoplasma Gondii ◽  
Strongyloides Stercoralis ◽  
Felis Catus ◽  
Gastrointestinal Parasites ◽  
Domestic Cats ◽  
Toxocara Cati ◽  
Fecal Samples ◽  
Dipylidium Caninum

Gastrointestinal (GIT) parasites of domestic cats (Felis catus) not only cause morbidity but are also potential zoonotic agents. The current study aimed at establishing the prevalence of GIT parasites in cats kept by households in Thika region, Kenya. Fecal samples were collected randomly from 103 cats and analyzed for presence of parasites using standard parasitological methods. In descending order, the prevalence of the detected protozoa parasites wasIsosporaspp. 43.7% (95% CI: 40.4–47%),Cryptosporidiumspp. 40.8% (95% CI: 37.5–44.1%),Toxoplasma gondii7.8% (95% CI: 4.5–11.1%), andEntamoebaspp. 2.9% (95% CI: 1.6–6.2%). The prevalence of the observed helminths wasStrongyloides stercoralis43.7% (95% CI: 40.4–47%),Toxocara cati23.3% (95% CI: 20–26.6%),Ancylostomaspp. 9.7% (95% CI: 6.4–13%),Dipylidium caninum8.7% (95% CI: 5.4–12.0%), andAcanthocephalaspp. 1.9% (95% CI: 1–4.2%). The percentage of cats excreting at least one species of parasite was 73.2% (95% CI = 69.9–76.5%). The study shows that the cats have high spectrum (9) of parasites which are known to affect the cat’s health and some are of zoonotic significance.

scholarly journals Prevalence and genetic characterization of Toxoplasma gondii in naturally infected backyard pigs intended for familial consumption in Romania

2019 ◽  
Vol 12 (1) ◽  
Author(s):  
Anamaria Ioana Paştiu ◽  
Anamaria Cozma-Petruț ◽  
Aurélien Mercier ◽  
Anamaria Balea ◽  
Lokman Galal ◽  
...  
Keyword(s):  
Toxoplasma Gondii ◽  
Rural Areas ◽  
Antibody Test ◽  
Antibody Detection ◽  
Serum Samples ◽  
Potential Threat ◽  
Polymerase Chain ◽  
Pcr Targeting ◽  
Backyard Pigs

Abstract Background Foodborne toxoplasmosis in humans can be due to the exposure to tissue cysts of Toxoplasma gondii through the consumption of meat, including pork, of infected animals. Traditional Romanian food habits include pork as the preferred meat, while backyard pig rearing remains a common practice in many rural areas of Romania. The aims of the present study were to estimate the prevalence of T. gondii infection in naturally infected backyard pigs slaughtered for familial consumption and to genetically characterize the T. gondii strains obtained. Methods Paired blood and heart samples were collected from 94 backyard pigs, home slaughtered for private consumption. Serum samples were analyzed using the immunofluorescence antibody test (IFAT) for anti-T. gondii antibody detection. Heart samples were screened by polymerase chain reaction (PCR) targeting the 529-bp repeat region (REP529) for T. gondii detection. In addition, heart samples from IFAT positive animals were bioassayed in mice. The T. gondii isolates were genotyped by the analysis of 15 microsatellite markers. Results The results showed that almost half of the pigs investigated were T. gondii seropositive (46.8%, 95% confidence interval (CI): 36.4–57.4%) and in more than a quarter of the pigs (26.6%, 95% CI: 18.0–36.7%), the parasite was detected by PCR. Three (3/44) T. gondii strains were isolated from hearts of seropositive pigs and they all belonged to genotype II. Conclusions The present study showed the presence of T. gondii infection in backyard pigs in Romania, which suggests that consumption of pork from animals reared and slaughtered at home may pose a potential threat to human health and should be given attention. In addition, to our knowledge, this is the first study to provide data concerning T. gondii strains circulating in pigs from Romania.

scholarly journals Prevalence and molecular characterization of Toxoplasma gondii DNA in retail fresh meats in Canada

2018 ◽  
Vol 13 ◽  
pp. e00031 ◽  
Author(s):  
Asma Iqbal ◽  
Nicol Janecko ◽  
Frank Pollari ◽  
Brent Dixon
Keyword(s):  
Toxoplasma Gondii ◽  
Molecular Characterization

Molecular characterization of TgMIC5, a proteolytically processed antigen secreted from the micronemes of Toxoplasma gondii

2000 ◽  
Vol 111 (1) ◽  
pp. 51-66 ◽  
Author(s):  
Susannah D Brydges ◽  
Gale D Sherman ◽  
Susanne Nockemann ◽  
Anne Loyens ◽  
Walter Däubener ◽  
...  
Keyword(s):  
Toxoplasma Gondii ◽  
Molecular Characterization

scholarly journals Isolation and Biological and Molecular Characterization of Toxoplasma gondii from Canine Cutaneous Toxoplasmosis in Brazil

2014 ◽  
Vol 52 (12) ◽  
pp. 4419-4420 ◽  
Author(s):  
H. F. J. Pena ◽  
L. R. Moroz ◽  
R. K. B. Sozigan ◽  
D. Ajzenberg ◽  
F. R. Carvalho ◽  
...  
Keyword(s):  
Toxoplasma Gondii ◽  
Molecular Characterization

Identification and molecular characterization of GRA8, a novel, proline-rich, dense granule protein of Toxoplasma gondii☆

2000 ◽  
Vol 105 (1) ◽  
pp. 25-37 ◽  
Author(s):  
Kimberly L. Carey ◽  
Carolyn G. Donahue ◽  
Gary E. Ward
Keyword(s):  
Toxoplasma Gondii ◽  
Molecular Characterization ◽  
Dense Granule ◽  
Granule Protein
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