SAG3 Toxoplasma gondii cloning reveals unexpected fivefold infection in the blood of feral cats in the Mexican Caribbean

Background Currently, more than 300 genotypes of Toxoplasma gondii (T. gondii) have been described throughout the world, demonstrating its wide genetic diversity. The SAG3 locus is one of the genes included in the genotyping panel of this parasite. It is associated with its virulence since it participates during the invasion process of the host cells. Therefore, cloning, sequencing, and bioinformatic analysis were used to deepen the understanding of the SAG3 locus genetic diversity of T. gondii in blood samples from feral cats. Results Six different SAG3 sequences were detected, five of which were detected in one feline. Three sequences were first reported here; one of them was an intragenic recombinant. In the cladogram, four out of ten SAG3 sequences did not share nodes with others reported worldwide. Conclusions Cloning and sequencing of samples with more than one restriction pattern by PCR-RFLP were very helpful tools to demonstrate the presence of more than three genotypes of T. gondii in the blood of feral cats from southeastern Mexico. This suggests a potential mixed infection of multiple T. gondii strains and high genetic diversity of the parasites in felines in this tropical region of Mexico.

ANALISIS PERILAKU MASYARAKAT PASAR MENGENAI PENGGUNAAN RODENTISIDA DAN DAMPAKNYA TERHADAP KUCING LIAR

Inappropriate use of rodenticides by most market communities has resulted in a reduction in the population of feral cats (Felis catus) found in the market. If this continues, it can lead to the extinction of feral cat species in the area. This study aims to describe the level of knowledge and attitudes of the market community regarding the use of rodenticides and their impact on feral cats (Felis catus). This study used a survey method, namely interviews and observations of 28 respondents who claimed to have used rodenticides. The results showed that 35.7% of respondents had a low level of knowledge, 39.3% had a moderate level of knowledge, and 25% had a high level of knowledge. As for the attitude aspect, the majority of the community obtained quite good criteria with an average of 68.

Two Methods of Monitoring Cats at a Landscape-Scale

Feral cats are difficult to manage and harder to monitor. We analysed the cost and the efficacy of monitoring the pre- and post-bait abundance of feral cats via camera-traps or track counts using four years of data from the Matuwa Indigenous Protected Area. Additionally, we report on the recovery of the feral cat population and the efficacy of subsequent Eradicat® aerial baiting programs following 12 months of intensive feral cat control in 2019. Significantly fewer cats were captured in 2020 (n = 8) compared to 2019 (n = 126). Pre-baiting surveys for 2020 and 2021 suggested that the population of feral cats on Matuwa was very low, at 5.5 and 4.4 cats/100 km, respectively, which is well below our target threshold of 10 cats/100 km. Post-baiting surveys then recorded 3.6 and 3.0 cats/100 km, respectively, which still equates to a 35% and 32% reduction in cat activity. Track counts recorded significantly more feral cats than camera traps and were cheaper to implement. We recommend that at least two methods of monitoring cats be implemented to prevent erroneous conclusions.

“State of the Mewnion”: Practices of Feral Cat Care and Advocacy Organizations in the United States

Over the last several decades, feral cats have moved from the fringes to the mainstream in animal welfare and sheltering. Although many best practice guidelines have been published by national non-profits and veterinary bodies, little is known about how groups “in the trenches” actually operate. Our study sought to address that gap through an online survey of feral cat care and advocacy organizations based in the United States. Advertised as “The State of the Mewnion,” its topics included a range of issues spanning non-profit administration, public health, caretaking and trapping, adoptions of friendly kittens and cats, veterinary medical procedures and policies, data collection and program efficacy metrics, research engagement and interest, and relationships with wildlife advocates and animal control agencies. Respondents from 567 organizations participated, making this the largest and most comprehensive study on this topic to date. Respondents came primarily from grassroots organizations. A majority reported no paid employees (74.6%), served 499 or fewer feral cats per year (75.0%), engaged between 1 and 9 active volunteers (54.9%), and did not operate a brick and mortar facility (63.7%). Some of our findings demonstrate a shared community of practice, including the common use of a minimum weight of 2.0 pounds for spay/neuter eligibility, left side ear tip removals to indicate sterilization, recovery holding times after surgery commonly reported as 1 night for male cats and 1 or 1 nights for females, requiring or recommending to adopters of socialized kittens/cats that they be kept indoor-only, and less than a quarter still engaging in routine testing of cats for FIV and FeLV. Our survey also reveals areas for improvement, such as most organizations lacking a declared goal with a measurable value and a time frame, only sometimes scanning cats for microchips, and about a third not using a standardized injection site for vaccines. This study paints the clearest picture yet available of what constitutes the standard practices of organizations serving feral and community cats in the United States.

Fishing for Feral Cats in a Naturally Fragmented Rocky Landscape Using Movement Data

Feral cats are one of the most damaging predators on Earth. They can be found throughout most of Australia’s mainland and many of its larger islands, where they are adaptable predators responsible for the decline and extinction of many species of native fauna. Managing feral cat populations to mitigate their impacts is a conservation priority. Control strategies can be better informed by knowledge of the locations that cats frequent the most. However, this information is rarely captured at the population level and therefore requires modelling based on observations of a sample of individuals. Here, we use movement data from collared feral cats to estimate home range sizes by gender and create species distribution models in the Pilbara bioregion of Western Australia. Home ranges were estimated using dynamic Brownian bridge movement models and split into 50% and 95% utilisation distribution contours. Species distribution models used points intersecting with the 50% utilisation contours and thinned by spacing points 500 m apart to remove sampling bias. Male cat home ranges were between 5 km2 (50% utilisation) and 34 km2 (95% utilisation), which were approximately twice the size of the female cats studied (2–17 km2). Species distribution modelling revealed a preference for low-lying riparian habitats with highly productive vegetation cover and a tendency to avoid newly burnt areas and topographically complex, rocky landscapes. Conservation management can benefit by targeting control effort in preferential habitat.

Landscape-Scale Effects of Fire, Cats, and Feral Livestock on Threatened Savanna Mammals: Unburnt Habitat Matters More Than Pyrodiversity

Northern Australia has undergone significant declines among threatened small and medium-sized mammals in recent decades. Conceptual models postulate that predation by feral cats is the primary driver, with changed disturbance regimes from fire and feral livestock in recent decades reducing habitat cover and exacerbating declines. However, there is little guidance on what scale habitat and disturbance attributes are most important for threatened mammals, and what elements and scale of fire mosaics actually support mammals. In this study, we test a series of hypotheses regarding the influence of site-scale (50 × 50 m) habitat and disturbance attributes, as well as local-scale (1 km radius), meta-local scale (3 km), landscape-scale (5 km) and meta-landscape scale (10 km) fire mosaic attributes on mammal abundance and richness. We found that habitat cover (rock, perennial grass, and shrub cover) at the site-scale had a positive effect, and disturbance factors (feral cats, fire, feral livestock) had a negative influence on mammal abundance and richness. Models supported site-scale habitat and disturbance factors as more important for mammals than broader-scale (local up to meta-landscape scale) fire mosaic attributes. Finally, we found that increasing the extent of ≥ 4 year unburnt habitat, and having an intermediate percentage (ca. 25%) of recently burnt (1-year burnt) habitat within the mosaic, were the most important functional elements of the fire mosaic at broad scales for mammals. Contrary to expectations, diversity of post-fire ages (‘pyrodiversity’) was negatively associated with mammal abundance and richness. These results highlight the need for management to promote retention of longer unburnt vegetation in sufficient patches across savanna landscapes (particularly of shrub and fruiting trees), maintain low-intensity patchy fire regimes, reduce the extent of intense late dry season wildfires, and to reduce the impact of feral livestock. This study provides further evidence for the role of feral cats in northern Australian mammal declines, and highlights the need for increased research into the efficacy of cat control methodologies in reducing biodiversity impacts in these extensive landscapes.

Multiple Methods of Monitoring Cats at a Landscape-scale

Feral cats are both difficult to manage and harder to monitor. We analysed the cost-efficacy of monitoring the pre- and post-bait abundance of feral cats via camera-traps or track counts using four years of data from the Matuwa Indigenous protected Area. Additionally, we report on the recovery of the feral cat population and the efficacy of subsequent Eradicat® aerial baiting programs following 12 months of intensive feral cat control in 2019 that consisted of aerial baiting and leg-hold trapping. Significantly fewer cats were captured in 2020 (n = 8) compared to 2019 (n = 126). Pre-baiting surveys for 2020 and 2021 suggested that the population of feral cats on Matuwa was very low, at 5.5 and 4.4 cats/100 km respectively, which is well below our target threshold of 10 cats/100 km. Post-baiting surveys then recorded 3.6 and 3.0 cats/100 km respectively, which still equates to a 35% and 32% reduction in cat activity. Track counts recorded significantly more feral cats than camera traps and were cheaper to implement. We recommend that at least two methods of monitoring cats be implemented to prevent erroneous conclusions.

Long-Distance Movements of Feral Cats in Semi-Arid South Australia and Implications for Conservation Management

Movements that extend beyond the usual space use of an animal have been documented in a range of species and are particularly prevalent in arid areas. We present long-distance movement data on five feral cats (Felis catus) GPS/VHF-collared during two different research projects in arid and semi-arid Australia. We compare these movements with data from other feral cat studies. Over a study period of three months in the Ikara-Flinders Ranges National Park, 4 out of 19 collared cats moved to sites that were 31, 41, 53 and 86 km away. Three of the cats were males, one female; their weight was between 2.1 and 4.1 kg. Two of the cats returned to the area of capture after three and six weeks. During the other study at Arid Recovery, one collared male cat (2.5 kg) was relocated after two years at a distance of 369 km from the area of collar deployment to the relocation area. The movements occurred following three years of record low rainfall. Our results build on the knowledge base of long-distance movements of feral cats reported at arid study sites and support the assertion that landscape-scale cat control programs in arid and semi-arid areas need to be of a sufficiently large scale to avoid rapid reinvasion and to effectively reduce cat density. Locally, cat control strategies need to be adjusted to improve coverage of areas highly used by cats to increase the efficiency of control operations.