scholarly journals U niqueness of the Gossypium mustelinum Genome Revealed by GISH and 45 S rDNA FISH

2013 ◽  
Vol 55 (7) ◽  
pp. 654-662 ◽  
Author(s):  
Qiong Wu ◽  
Fang Liu ◽  
Shaohui Li ◽  
Guoli Song ◽  
Chunying Wang ◽  
...  
Keyword(s):  
Gossypium Mustelinum ◽  
Rdna Fish

Karyotype, banding and rDNA FISH in the scarab beetle Anoplotrupes stercorosus (Coleoptera Scarabaeoidea: Geotrupidae). Description and comparative analysis

Micron ◽  
2004 ◽  
Vol 35 (8) ◽  
pp. 717-720 ◽  
Author(s):  
Mariastella Colomba ◽  
Roberto Vitturi ◽  
Nicola Volpe ◽  
Antonella Lannino ◽  
Mario Zunino
Keyword(s):  
Comparative Analysis ◽  
Scarab Beetle ◽  
Rdna Fish

scholarly journals Identification and Characterization of Satellite DNAs in Poa L.

2020 ◽  
Author(s):  
Linna Wei ◽  
Bo Liu ◽  
Chunping Zhang ◽  
Yang Yu ◽  
Xiaoxia Yang ◽  
...  
Keyword(s):  
5S Rdna ◽  
Plant Genome ◽  
Satellite Dnas ◽  
Hybridization Intensity ◽  
Large Genus ◽  
Multiple Regions ◽  
Rdna Fish ◽  
Main Components ◽  
Definition Of ◽  
Relationship Of

Abstract BackgroundPoa L is a large genus of grass in Gramineae, among which P. pratensis is widely cultivated as turf and forage. Satellite DNA is the main components of the plant genome. Information of satellites will helpful for dissection the genome composition and definition of the phylogeny relationship of these species. However, the knowledge about the satellites in genus Poa is still limited.ResultsFour satellite DNAs were identified using the Repeat Explorer pipeline in HiSeq Illumina reads from diploid plants in P. malaca (2n = 26). Two satellites showed high similarity with the previously identified PpTr-1 and PpTr-3, whereas two others are newly identified with the monomer of 326bp (Poa-326) and 353bp (Poa-353) respectively. The clone DNAs of PpTr-1 and PpTr-3, and oligonucleotides designed representing satellites Poa-326 and Poa-353 were probed to test on chromosomes across 13 Poa speceis with different polyploidy level by fluorescent in situ hybridization (FISH). PpTr-1, PpTr-3, and Poa-362 were stably positioned in the subtelomeric regions in nearly all species with the variation of hybridization sites number. However, Poa-353 showed different FISH patterns of multiple regions with the variation of hybridization intensity and distribution sites across species. In addition, 5S rDNA and 45S rDNA were used to characterize the genome of the Poa species. Four rDNA FISH patterns were revealed in the tested species.ConclusionFour identified satellite were high conservable across Poa species. Genome distribution of these satellites can be characterized by FISH. The variation of satellite DNAs and rDNA chromosomal distributions between species provide useful information for phylogenetic analysis in genus Poa.

Phaeophytins from Gossypium mustelinum Miers ex Watt (Malvaceae)

2006 ◽  
Vol 34 (3) ◽  
pp. 263-264 ◽  
Author(s):  
T.M.S. Silva ◽  
C.A. Camara ◽  
F.D. Medeiros ◽  
E.J. Oliveira ◽  
M.F. Agra ◽  
...  
Keyword(s):  
Gossypium Mustelinum

scholarly journals Diversity and genetic structure among subpopulations of Gossypium mustelinum (Malvaceae)

2013 ◽  
Vol 12 (1) ◽  
pp. 597-609 ◽  
Author(s):  
M.F. Alves ◽  
P.A.V. Barroso ◽  
A.Y. Ciampi ◽  
L.V. Hoffmann ◽  
V.C.R. Azevedo ◽  
...  
Keyword(s):  
Genetic Structure ◽  
Gossypium Mustelinum

scholarly journals The lowest diploid number in Testudines: Banding patterns, telomeric and 45S rDNA FISH in Peltocephalus dumerilianus, 2n = 26 and FN = 52 (Pleurodira, Podocnemididae)

2014 ◽  
Vol 37 (1) ◽  
pp. 61-63 ◽  
Author(s):  
Karen Ventura ◽  
Camila N Moreira ◽  
Renata Moretti ◽  
Yatiyo Yonenag-Yassuda ◽  
Miguel T Rodrigues
Keyword(s):  
Diploid Number ◽  
45S Rdna ◽  
Banding Patterns ◽  
Rdna Fish

Karyotype analysis of Nicotiana kawakamii Y. Ohashi using DAPI banding and rDNA FISH

2001 ◽  
Vol 102 (6-7) ◽  
pp. 810-814 ◽  
Author(s):  
R. Nakamura ◽  
S. Kitamura ◽  
M. Inoue ◽  
N. Ohmido ◽  
K. Fukui
Keyword(s):  
Karyotype Analysis ◽  
Dapi Banding ◽  
Rdna Fish

scholarly journals Chromosomal characterization of the bonytongue Arapaima gigas (Osteoglossiformes: Arapaimidae)

2006 ◽  
Vol 4 (2) ◽  
pp. 215-218 ◽  
Author(s):  
Debora Karla Marques ◽  
Paulo Cesar Venere ◽  
Pedro Manoel Galetti Junior
Keyword(s):  
Sex Chromosome ◽  
18S Rrna Gene ◽  
Rrna Gene ◽  
Gene Probe ◽  
Mitotic Chromosomes ◽  
Araguaia River ◽  
Arapaima Gigas ◽  
Rdna Fish

The mitotic chromosomes of the pirarucu Arapaima gigas inhabiting the middle Araguaia River and collected in the municipality of Araguaiana (MT, Brazil) were studied. The chromosomes were analyzed through Giemsa staining, C-banding, Ag-NOR staining and in situ hybridization using an 18S rRNA gene probe. The karyotype had 2n=56 comprising 14 biarmed and 14 uniarmed chromosome pairs in both sexes. No cytologically distinguishable sex chromosome was identified. A single NOR-bearing chromosome pair was detected by Ag-NOR staining and confirmed by 18S rDNA- FISH. Faint constitutive heterochromatin was C-banded in the centromeric region of some chromosomes.

scholarly journals Contributions to the systematic of Pimelodidae (Osteichthyes, Siluriformes): basic and molecular cytogenetics on seven species of Pimelodus from three Brazilian hydrographic systems

2018 ◽  
Vol 16 (2) ◽  
Author(s):  
Simone C. Girardi ◽  
Carla S. Pavanelli ◽  
Vladimir P. Margarido
Keyword(s):  
18S Rdna ◽  
Molecular Cytogenetics ◽  
5S Rdna ◽  
Neotropical Region ◽  
Valid Species ◽  
Rdna Sites ◽  
The Family ◽  
Rdna Fish ◽  
Pimelodus Maculatus ◽  
Cytogenetic Methods

ABSTRACT Pimelodidae harbors several species and is widely distributed throughout the Neotropical region. Pimelodus is the genus with the largest number of species, however it is a polyphyletic group. Cytogenetic analyzes of the valid species still covers less than half of them. Herein, seven Pimelodus species from three Brazilian hydrographic systems were analyzed through basic (Giemsa, AgNORs and C banding) and molecular (5S and 18S rDNA-FISH) cytogenetic methods. All species had 2n=56 chromosomes with different karyotype formulas observed among the species. AgNORs were corresponding to 18S rDNA and localized on long arm of one chromosome pair in all species. Heterochromatin distribution follows the pattern commonly verified in the family and allows to identify each one of the studied species. 5S rDNA marker was interspecifically variable in number and position of cistrons. Pimelodus ortmanni had B chromosomes varying intra and inter-individually. We performed a discussion on our own and available cytogenetic data for Pimelodidae, and the associating of them with available phylogeny enable us identifying features that distinguish subgroups within Pimelodidae, such as NORs location (terminal/long arm for species belonging to “Iheringichthys-Parapimelodus” and “Pimelodus maculatus” subclades) and location of 5S rDNA sites (pericentromeric/interstitial/ long arm for species belonging to Pimelodus group).

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