Delta-like protein 1 in the pituitary-adipose axis in the adult male mouse

As a preliminary study of the conditions which affect the hour to hour variations in epidermal mitotic activity in the adult male mouse, experiments were carried out involving artificially induced sleep and exercise. In the first experiment the animals were injected with a dose of barbiturates sufficient to induce sleep for a period of 3 or 4 hr. at a time of day when they were usually active. The injections were given at 17.00 hr. as the animals were waking from their afternoon sleep, and they resulted in a sharp rise in mitotic activity. A maximum was reached at 20.00 hr., the time when the mitotic activity of the controls had dropped to a minimum. As the animals recovered and became active, their mitosis rate fell quickly to a low level. In the second experiment the animals were placed in a slowly revolving box, and so forced to remain awake and active throughout an afternoon when they would otherwise have been asleep. This treatment resulted in extremely low mitotic activity, in contrast to which that of the normally sleeping controls rose to a high level. It is probably significant that when the mice were released from the revolving box, and could at last sleep, their mitosis rate remained low. This suggests that the excessive exercise had either resulted in the production of some mitosis-depressing substance which remained in the system, or that some substance vital to mitosis had been used up and took some time to be replaced. The conclusion is now justified that the rate of epidermal mitosis normally increases during sleep, and decreases during hours of wakefulness and exercise. In this way the form of the diurnal mitosis cycle is determined by the habits of the animals.

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Faculty Opinions recommendation of Maternal diet-induced obesity programs cardiovascular dysfunction in adult male mouse offspring independent of current body weight.

Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature ◽

10.3410/f.718502149.793500301 ◽

2014 ◽

Author(s):

Blesson Chellakkan Selvanesan

Keyword(s):

Body Weight ◽

Adult Male ◽

Male Mouse ◽

Maternal Diet ◽

Cardiovascular Dysfunction ◽

Diet Induced Obesity ◽

Adult Male Mouse

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Novel sex-dependent differentially methylated regions are demethylated in adult male mouse livers

Biochemical and Biophysical Research Communications ◽

10.1016/j.bbrc.2015.04.137 ◽

2015 ◽

Vol 462 (4) ◽

pp. 332-338 ◽

Cited By ~ 5

Author(s):

Shuhei Ito ◽

Keiji Hirabayashi ◽

Kohji Moriishi ◽

Yasuhisa Matsui ◽

Kyoji Moriya ◽

...

Keyword(s):

Adult Male ◽

Male Mouse ◽

Differentially Methylated Regions ◽

Adult Male Mouse

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Mitotic activity in the adult male mouse, Mus musculus L. The diurnal cycles and their relation to waking and sleeping

Proceedings of the Royal Society of London Series B Biological Sciences ◽

10.1098/rspb.1948.0007 ◽

1948 ◽

Vol 135 (879) ◽

pp. 212-233 ◽

Cited By ~ 86

Keyword(s):

Cell Division ◽

Mitotic Activity ◽

Adult Male ◽

Individual Variation ◽

Male Mouse ◽

Duodenal Mucosa ◽

Seminiferous Tubules ◽

Diurnal Cycles ◽

Adult Male Mouse ◽

Bodily Activity

The diurnal cycles of mitotic activity in the ear epidermis of the adult male mouse have been determined by the removal of earclips at 2 hr. intervals throughout the 24 hr. The mice used were between 3 and 4 months old, and were of the Kreyberg white label and Strong’s CBA strains. A considerable degree of individual variation was found, but on the average the maximum mitotic activity was at 06.00 and 14.00 hr. and the minimum mitotic activity at 10.00 and 20.00 hr. This observation was confirmed by killing groups of mice, each group consisting of five males, at the same 2 hr. intervals throughout the 24 hr. Similar variations in the mitotic activity of the ear epidermis were observed, and, in addition, similar cycles were evident in the mid-dorsal epidermis of the back, the stratified epithelium of the oesophagus, the lining epithelium of the epididymis, and the proliferating zone of the duodenal mucosa. In this last tissue the rate of cell division never fell to a very low figure, and in the proliferating centres of the intestinal lymph nodules and in the seminiferous tubules of the testis there was no trace of a cycle since the rate of cell division remained constantly high. A study was also made of the spontaneous bodily activity of the mice throughout the 24 hr., and by comparing the average figures so obtained with the average figures for epidermal mitosis, it proved possible to make the significant correlation that when the animals are at rest mitotic activity is at a maximum and that when they are awake and active it is at a minimum. This correlation permits an explanation of the individual variation in mitotic activity, since there is also a high degree of individual variation in spontaneous bodily activity. It also permits an explanation of the contradictory results which have been reported in the past regarding diurnal mitosis rhythms in mice, since it is evident that the rhythms of bodily activity must be strongly affected by differences in the age, sex and condition of the animals used, in the season of the year, and in the routine of the laboratory.

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