Porphyromonas gingivalis lipopolysaccharide induces over production of CC chemokine ligand 2 via toll-like receptor-4 in oral lichen planus

2017 ◽  
Vol 47 (2) ◽  
pp. 166-172 ◽  
Author(s):  
Qi Zeng ◽  
Xi Yang ◽  
Xiaobing Chen ◽  
Juan Xia ◽  
Bin Cheng ◽  
...  
Keyword(s):  
Lichen Planus ◽  
Porphyromonas Gingivalis ◽  
Oral Lichen Planus ◽  
Toll Like Receptor ◽  
Toll Like Receptor 4 ◽  
Chemokine Ligand ◽  
Porphyromonas Gingivalis Lipopolysaccharide ◽  
Cc Chemokine Ligand 2 ◽  
Chemokine Ligand 2 ◽  
Oral Lichen

Downregulation of toll-like receptor-mediated signalling pathways in oral lichen planus

2015 ◽  
Vol 45 (1) ◽  
pp. 28-34 ◽  
Author(s):  
Suraya H. Sinon ◽  
Alison M. Rich ◽  
Venkata P. B. Parachuru ◽  
Fiona A. Firth ◽  
Trudy Milne ◽  
...  
Keyword(s):  
Lichen Planus ◽  
Oral Lichen Planus ◽  
Signalling Pathways ◽  
Toll Like Receptor ◽  
Oral Lichen

CC chemokine ligand 2 down-modulation by selected Toll-like receptor agonist combinations contributes to T helper 1 polarization in human dendritic cells

Blood ◽  
2009 ◽  
Vol 114 (4) ◽  
pp. 796-806 ◽  
Author(s):  
Manuela Del Cornò ◽  
Alessandro Michienzi ◽  
Andrea Masotti ◽  
Letizia Da Sacco ◽  
Gian Franco Bottazzo ◽  
...  
Keyword(s):  
Dendritic Cells ◽  
Interleukin 12 ◽  
Toll Like Receptor ◽  
T Helper ◽  
Cc Chemokine ◽  
Chemokine Ligand ◽  
Cc Chemokine Ligand 2 ◽  
Chemokine Ligand 2 ◽  
Helper Type

Abstract Toll-like receptor (TLR) signaling activation by pathogens is critical to the induction of immune responses, and demands tight regulation. We describe in this study that CC chemokine ligand 2 (CCL2) secretion triggered by TLR4 or TLR8 engagement is strongly inhibited upon simultaneous activation of both TLRs in human monocyte-derived dendritic cells (DCs). Impaired CCL2 secretion occurs concomitantly to interleukin-12 up-regulation, being part of a complex regulatory circuit ensuring optimal T helper type 1 polarization. Interestingly, triggering selected TLRs or their combinations differently affects nuclear factor-κB p65 activation and microRNA expression. Overall, these results indicate that CCL2 supplies an important immunomodulatory role to DCs, and may contribute to dictate the cytokine profile in T helper type 1 responses induced by DCs.

Cementoblast Gene Expression is Regulated by Porphyromonas gingivalis Lipopolysaccharide Partially via Toll-like Receptor-4/MD-2

2004 ◽  
Vol 83 (8) ◽  
pp. 602-607 ◽  
Author(s):  
F.H. Nociti ◽  
B.L. Foster ◽  
S.P. Barros ◽  
R.P. Darveau ◽  
M.J. Somerman
Keyword(s):  
Gene Expression ◽  
Porphyromonas Gingivalis ◽  
Cell Function ◽  
Nuclear Factor Κb ◽  
Mrna Levels ◽  
Toll Like Receptor ◽  
Toll Like Receptor 4 ◽  
Periodontal Tissues ◽  
Mineralized Tissues ◽  
Porphyromonas Gingivalis Lipopolysaccharide

Lipopolysaccharides are potent inflammatory mediators considered to contribute to destruction of periodontal tissues. Here, we hypothesized that Porphyromonas gingivalis lipopolysaccharide (P-LPS) treatment would regulate gene expression in murine cementoblasts through Toll-like receptor 4. Real-time (RT)-PCR and Northern blot analysis indicated that P-LPS decreased expression of transcripts for osteocalcin (OCN) and receptor activator of nuclear factor κB ligand (RANKL). In contrast, a dose-dependent up-regulation in mRNA levels for osteopontin (OPN) and osteoprotegerin (OPG) was observed. Similarly, ELISA demonstrated decreased RANKL and increased OPG levels. A monoclonal antibody specific for mouse TLR-4/MD-2 partially neutralized the P-LPS effect on cementoblasts. These results indicate that exposure of cementoblasts to P-LPS can alter cell function by regulating markers of osteoclastic activity ( e.g., RANKL/OPG), thereby potentially affecting the inflammation-associated resorption of mineralized tissues.

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