DNA barcodes and microsatellites: how they complement for species identification in the complex genus Tamarix (Tamaricaceae)

DNA barcoding has been recently promoted as a method for both assigning specimens to known species and for discovering new and cryptic species. Here we test both the potential and the limitations of DNA barcodes by analysing a group of well-studied organisms—the primates. Our results show that DNA barcodes provide enough information to efficiently identify and delineate primate species, but that they cannot reliably uncover many of the deeper phylogenetic relationships. Our conclusion is that these short DNA sequences do not contain enough information to build reliable molecular phylogenies or define new species, but that they can provide efficient sequence tags for assigning unknown specimens to known species. As such, DNA barcoding provides enormous potential for use in global biodiversity studies.Key words: DNA barcoding, species identification, primate, biodiversity.

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BarcodingR: an integrated r package for species identification using DNA barcodes

Methods in Ecology and Evolution ◽

10.1111/2041-210x.12682 ◽

2016 ◽

Vol 8 (5) ◽

pp. 627-634 ◽

Cited By ~ 19

Author(s):

Ai‐bing Zhang ◽

Meng‐di Hao ◽

Cai‐qing Yang ◽

Zhi‐yong Shi

Keyword(s):

Species Identification ◽

R Package ◽

Dna Barcodes

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The effect of sampling from subdivided populations on species identification with DNA barcodes using a Bayesian statistical approach

Molecular Phylogenetics and Evolution ◽

10.1016/j.ympev.2012.07.033 ◽

2012 ◽

Vol 65 (2) ◽

pp. 765-773 ◽

Cited By ~ 12

Author(s):

Melanie Lou ◽

G. Brian Golding

Keyword(s):

Species Identification ◽

Statistical Approach ◽

Dna Barcodes ◽

Subdivided Populations

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Comprehensive DNA barcodes for species identification and discovery of cryptic diversity in mayfly larvae from South Korea: Implications for freshwater ecosystem biomonitoring

Entomological Research ◽

10.1111/1748-5967.12334 ◽

2019 ◽

Vol 49 (1) ◽

pp. 46-54 ◽

Cited By ~ 2

Author(s):

Kyong In Suh ◽

Jeong Mi Hwang ◽

Yeon Jae Bae ◽

Ji Hyoun Kang

Keyword(s):

South Korea ◽

Species Identification ◽

Freshwater Ecosystem ◽

Cryptic Diversity ◽

Dna Barcodes

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Using DNA barcodes to assess identity and diversity of Dendropsophus minutus: Failure?

Zootaxa ◽

10.11646/zootaxa.1691.1.3 ◽

2008 ◽

Vol 1691 (1) ◽

pp. 67 ◽

Cited By ~ 3

Author(s):

M. ALEX SMITH

Keyword(s):

Cytochrome C ◽

Small Group ◽

Cytochrome C Oxidase ◽

Species Identification ◽

Mitochondrial Gene ◽

Dna Barcode ◽

Gene Region ◽

Dna Barcodes ◽

Taxonomic Groups

The 5' end (Folmer or Barcode region) of cytochrome c oxidase 1 (CO1) has been proposed as the gene region of choice for a standardized animal DNA barcode (Hebert et al. 2003). Concerns have been raised regarding the decision to utilize this particular mitochondrial gene region as a barcode. Nevertheless, widely divergent taxonomic groups have reported success using CO1 for both species identification and discovery. The utility of CO1 for barcoding amphibians was raised early on (Vences, et al. 2005) and concerns for this group were reported widely (Waugh 2007)—although some considered that the reporting of the concerns outstripped the data that had been analyzed at that point (Smith et al. 2008). Indeed, our analysis of CO1 for a small group of Holarctic amphibians was neither more difficult to generate nor to analyze than for other groups where we have utilized the technique.

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