scholarly journals Establishment of an International Reference Reagent for standardization of von Willebrand factor binding to recombinant glycoprotein Ib (VWF:GPIbM and VWF:GPIbR)

2019 ◽  
Vol 17 (6) ◽  
pp. 1003-1005 ◽  
Author(s):  
Anthony R. Hubbard ◽  
Sandra L. Haberichter ◽  
Keyword(s):  
Von Willebrand Factor ◽  
Glycoprotein Ib ◽  
Recombinant Glycoprotein ◽  
International Reference ◽  
Factor Binding ◽  
Von Willebrand ◽  
Willebrand Factor

scholarly journals Fibrinogen competes with von Willebrand factor for binding to the glycoprotein IIb/IIIa complex when platelets are stimulated with thrombin

Blood ◽  
1984 ◽  
Vol 64 (4) ◽  
pp. 797-800 ◽  
Author(s):  
HR Gralnick ◽  
SB Williams ◽  
BS Coller
Keyword(s):  
Monoclonal Antibodies ◽  
Von Willebrand Factor ◽  
Adenosine Diphosphate ◽  
Glycoprotein Ib ◽  
Human Fibrinogen ◽  
Factor Binding ◽  
Normal Plasma ◽  
Platelet Binding ◽  
Von Willebrand ◽  
Willebrand Factor

Two monoclonal antibodies--one that blocks ristocetin-induced platelet binding of von Willebrand factor to glycoprotein Ib and one that blocks adenosine diphosphate-induced binding of fibrinogen to the glycoprotein IIb/IIIa complex--were used to assess the binding site(s) for von Willebrand factor when platelets are stimulated with thrombin or adenosine diphosphate (ADP). Neither agonist induced binding of von Willebrand factor to glycoprotein Ib. ADP and thrombin induced von Willebrand factor binding exclusively to the glycoprotein IIb/IIIa complex. The results of the site of binding of von Willebrand factor with thrombasthenic platelets were consistent with the data obtained with the monoclonal antibodies and normal platelets. Human fibrinogen caused complete inhibition of thrombin-induced von Willebrand factor binding to normal platelets at concentrations considerably below that found in normal plasma. We conclude that thrombin induces very little binding of exogenous von Willebrand factor to platelets at normal plasma fibrinogen levels.

Direct demonstration of radiolabeled von willebrand factor binding to platelet glycoprotein Ib and IIb-IIIa in the presence of shear stress

1995 ◽  
Vol 23 (6) ◽  
pp. 787-793 ◽  
Author(s):  
Jeffrey K. McCrary ◽  
Leticia H. Nolasco ◽  
J. David Hellums ◽  
Michael H. Kroll ◽  
Nancy A. Turner ◽  
...  
Keyword(s):  
Shear Stress ◽  
Von Willebrand Factor ◽  
Platelet Glycoprotein ◽  
Glycoprotein Ib ◽  
Factor Binding ◽  
Direct Demonstration ◽  
Von Willebrand ◽  
Willebrand Factor
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