scholarly journals A method of direct PCR without DNA extraction for rapid detection of begomoviruses infecting jute and mesta

2013 ◽  
Vol 58 (4) ◽  
pp. 350-355 ◽  
Author(s):  
C. Biswas ◽  
P. Dey ◽  
S. Satpathy
Keyword(s):  
Dna Extraction ◽  
Rapid Detection ◽  
Direct Pcr

scholarly journals Direct PCR from whole blood, without DNA extraction

1990 ◽  
Vol 18 (19) ◽  
pp. 5908-5909 ◽  
Author(s):  
B. Mercier ◽  
C. Gaucher ◽  
O. Feugeas ◽  
C. Mazurier
Keyword(s):  
Dna Extraction ◽  
Whole Blood ◽  
Direct Pcr

scholarly journals Detection of Bacteria in Environmental Samples by Direct PCR Without DNA Extraction

BioTechniques ◽  
2001 ◽  
Vol 31 (3) ◽  
pp. 598-607 ◽  
Author(s):  
Kimberly A. Fode-Vaughan ◽  
Charles F. Wimpee ◽  
Charles C. Remsen ◽  
Mary Lynne Perille Collins
Keyword(s):  
Dna Extraction ◽  
Environmental Samples ◽  
Direct Pcr

Direct Diagnosis of Trichomonas vaginalis Infection on Archived Pap Smears Using Nested PCR

2015 ◽  
Vol 59 (1) ◽  
pp. 104-108 ◽  
Author(s):  
Hajar Ziaei Hezarjaribi ◽  
Mahbobeh Taghavi ◽  
Mahdi Fakhar ◽  
Shirzad Gholami
Keyword(s):  
Dna Extraction ◽  
Study Design ◽  
Nested Pcr ◽  
Trichomonas Vaginalis ◽  
Direct Detection ◽  
Urogenital Tract ◽  
Pap Smears ◽  
Direct Pcr ◽  
Parasitic Protozoan ◽  
Archived Samples

Objectives: Little information is available concerning PCR-based direct detection of Trichomonas infections on archived Pap (Papanicolaou)-stained smears. This study investigates DNA extraction and amplification from archived Pap smears. Trichomonas vaginalis is a parasitic protozoan that infects the urogenital tract of women. Study Design: DNA from archived Pap-stained smears was successfully amplified using the nested PCR to investigate if it could be used for accurate detection and retrospective epidemiological investigations. Results: In our study, 98 (75.4%) out of 130 specimens of T. vaginalis Pap-stained smears were found to be positive by the nested PCR. Also, direct PCR on the archived Pap smears for identifying T. vaginalis gave a specificity of 100%. Conclusion: PCR-based Pap smears appear to offer an effective method to detect Trichomonas infection in archived samples, being rapid, highly specific and convenient for sampling, particularly in retrospective investigations.

Simple ‘universal’ DNA extraction procedure compatible with direct PCR amplification

1996 ◽  
Vol 52 (4) ◽  
pp. 295-295 ◽  
Author(s):  
D. Goldenberger ◽  
I. Perschil ◽  
M. Ritzler ◽  
M. Altwegg
Keyword(s):  
Dna Extraction ◽  
Extraction Procedure ◽  
Pcr Amplification ◽  
Direct Pcr

Rapid detection of Phytophthora nicotianae by simple DNA extraction and real-time loop-mediated isothermal amplification assay

2019 ◽  
Vol 167 (3) ◽  
pp. 174-184 ◽  
Author(s):  
Ayaka Hieno ◽  
Mingzhu Li ◽  
Auliana Afandi ◽  
Kayoko Otsubo ◽  
Haruhisa Suga ◽  
...  
Keyword(s):  
Real Time ◽  
Dna Extraction ◽  
Rapid Detection ◽  
Isothermal Amplification ◽  
Phytophthora Nicotianae ◽  
Loop Mediated Isothermal Amplification ◽  
Amplification Assay ◽  
Time Loop

scholarly journals ‘Direct PCR’ optimization yields a rapid, cost-effective, nondestructive and efficient method for obtaining DNA barcodes without DNA extraction

2014 ◽  
Vol 14 (6) ◽  
pp. 1271-1280 ◽  
Author(s):  
Wing Hing Wong ◽  
Ywee Chieh Tay ◽  
Jayanthi Puniamoorthy ◽  
Michael Balke ◽  
Peter S. Cranston ◽  
...  
Keyword(s):  
Dna Extraction ◽  
Efficient Method ◽  
Cost Effective ◽  
Dna Barcodes ◽  
Direct Pcr ◽  
Pcr Optimization

Simple and Rapid Detection of Salmonella by Direct PCR Amplification of Gene fimW

2014 ◽  
Vol 69 (4) ◽  
pp. 429-435 ◽  
Author(s):  
Jiang-ying Zhang ◽  
Li-wei Dong ◽  
Qian Ren ◽  
Xiao-zhou Wang ◽  
Yi Yang ◽  
...  
Keyword(s):  
Rapid Detection ◽  
Pcr Amplification ◽  
Direct Pcr
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