Specific PCR‐based detection of Phomopsis vexans the cause of leaf blight and fruit rot pathogen of Solanum melongena L.

2019 ◽  
Vol 69 (5) ◽  
pp. 358-365
Author(s):  
A.C. Udayashankar ◽  
S. Chandra Nayaka ◽  
B. Archana ◽  
T.R. Lakshmeesha ◽  
S.R. Niranjana ◽  
...  
Keyword(s):  
Solanum Melongena ◽  
Leaf Blight ◽  
Fruit Rot ◽  
Specific Pcr ◽  
Phomopsis Vexans

scholarly journals PCR based detection of Phomopsis vexans (Sacc. & Syd.) - The causative agent of leaf blight and fruit rot disease of Brinjal (Solanum melongena L.)

2013 ◽  
Vol 7 (1) ◽  
pp. 17-20 ◽  
Author(s):  
KM Jayaramaiah ◽  
S. Mahadevakumar ◽  
AP Charith Raj ◽  
GR Janardhana
Keyword(s):  
Solanum Melongena ◽  
Culture Method ◽  
Leaf Blight ◽  
Fruit Rot ◽  
Universal Primer ◽  
Conventional Culture ◽  
Detection Assay ◽  
Pcr Products ◽  
Phomopsis Vexans ◽  
Rot Disease

Leaf blight and fruit rot disease of brinjal (Solanum melongena) caused by the fungal pathogen Phomopsis vexans is the most prevailing diseases responsible for the significant yield loss in many Brinjal growing regions of India. The survey conducted during 2008-2010, seven (07) isolates of Phomopsis vexans causing leaf blight and fruit rot disease in different areas of Karnataka were isolated, identified and confirmed by Koch’s postulates. Identification of Phomopsis vexans by conventional culture method was time consuming and laborious. Hence, PCR based detection assay was used to detect the pathogen from DNA samples obtained from fungal isolates. Universal primer pairs designed from internal transcribed spacer regions ITS1 (5'-CGGATCTCTTGGTTCTGGCA-3') and ITS4 (5'-GACGCTCGAACAGGCATGCC-3') of the ribosomal DNA (rDNA) of the genus Phomopsis were used for PCR. The study revealed the amplification of expected 553bp PCR products in all the DNA samples isolated from different isolates of Phomopsis vexans confirming their association in leaf blight and fruit rot disease of Brinjal.DOI: http://dx.doi.org/10.3126/ijls.v7i1.8173 International Journal of Life Sciences 7(1): 2013; 17-20

Phomopsis vexans (Phomopsis blight of eggplant).

2021 ◽  
Author(s):  
Keyword(s):  
Solanum Melongena ◽  
Seed Transmission ◽  
Fruit Rot ◽  
Damping Off ◽  
Cultural Methods ◽  
Large Numbers ◽  
Historical Distribution ◽  
Poor Seed ◽  
Phomopsis Vexans ◽  
Stem Lesions

Abstract P. vexans is a pynicidial anamorph with a teleomorph in the genus Diaporthe. Easily seedborne and producing large numbers of conidia, it causes disease in Solanum melongena [aubergine/brinjal/eggplant], its only significant host. This ranges from poor seed germination and damping-off of seedlings, to leaf and stem lesions and to fruit rot, both in the field and after harvest. The fungus has been reported from widely distributed areas of most continents, but only a few of those are in Europe and Africa, even though the climates are favourable. Seed transmission may explain its broad historical distribution, but limitation of its host range to a non-staple vegetable crop can allow for its avoidance and eradication by cultural methods. As a result, perhaps, it does not appear often on lists of restricted pathogens, even though it may cause yield losses of more than 50%.

Morpho-cultural and pathological variability in Phomopsis vexans causing leaf blight and fruit rot of brinjal in Kashmir

2019 ◽  
Vol 72 (2) ◽  
pp. 225-233
Author(s):  
Mudasir Bhat ◽  
Ali Anwar ◽  
M. Najeeb Mughal ◽  
Fayaz Mohiddin ◽  
Mi Makhdoomi ◽  
...  
Keyword(s):  
Leaf Blight ◽  
Fruit Rot ◽  
Phomopsis Vexans

scholarly journals Correction to: Morpho-cultural and pathological variability in Phomopsis vexans causing leaf blight and fruit rot of brinjal in Kashmir

2019 ◽  
Vol 72 (3) ◽  
pp. 561-561
Author(s):  
Mudasir Bhat ◽  
Ali Anwar ◽  
M. Najeeb Mughal ◽  
Fayaz Mohiddin ◽  
Mi Makhdoomi ◽  
...  
Keyword(s):  
Leaf Blight ◽  
Fruit Rot ◽  
Phomopsis Vexans

Phomopsis vexans. [Descriptions of Fungi and Bacteria].

1972 ◽  
Author(s):  
E. Punithalingam
Keyword(s):  
Geographical Distribution ◽  
West Indies ◽  
Solanum Melongena ◽  
Fruit Rot ◽  
Primary Sources ◽  
Damping Off ◽  
The West ◽  
Leaf Spots ◽  
Phomopsis Vexans ◽  
Stem Lesions

Abstract A description is provided for Phomopsis vexans[Diaporthe vexans]. Information is included on the disease caused by the organism, its transmission, geographical distribution, and hosts. HOSTS: On Solanum melongena, S. wendlandii. DISEASE: The only economic host is eggplant or brinjal (Solanum melongena) and the disease is variously known as tip over, stem blight or canker, leaf blight or spot and fruit rot; damping-off can also take place. Leaf spots (up to 3 cm diam.) are conspicuous, irregular in outline and may coalesce; lower leaves may be affected first. In stem lesions the cortex dries and cracks, plants become stunted and girdling cankers cause death. Fruit spots are pale, sunken, conspicuous and may affect the whole fruit; fruit may drop or remain attached, becoming mummified after a soft decay. Pycnidia are abundant. GEOGRAPHICAL DISTRIBUTION: Widespread in N. America, the West Indies, and E. and central Asia, also in Africa (Senegal, Tanzania, Zambia) and Mauritius (CMI Map 329, ed. 2, 1968). The record from Australia (NSW) should be deleted. Additional records not yet mapped are: Brunei, Haiti, Iran, Iraq, Rumania. TRANSMISSION: Host debris and seed from infested fruit are primary sources of inoculum. Naturally infected seed germinates less well and more slowly (20: 621; 22: 511).

scholarly journals THE EGGPLANT BLIGHT AND FRUIT ROT IN PORTO RICO

1969 ◽  
Vol 13 (2) ◽  
pp. 35-57
Author(s):  
J. A. B. Nolla
Keyword(s):  
Bordeaux Mixture ◽  
Control Measure ◽  
Soil Surface ◽  
The United States ◽  
Labor Cost ◽  
Fruit Rot ◽  
Seedling Blight ◽  
Stem Blight ◽  
Phomopsis Vexans ◽  
Serious Disease

1. A serious disease of eggplants known in Porto Rico as "lunares de la hoja y tallo" and "podredumhre de la fruta", in the United States of North America as leaf blight, foot-rot, leaf-spot, stem-blight. fruit-rot, eggplant-blight and seedling-stem-blight and in Cuba as "mancha de la hoja" and "enfermedad del tallo" exists in Porto Rico. 2. All varieties of eggplant are more or less equally susceptible under Porto Rican conditions. Color of plant or of fruit has no bearing on susceptibility or resistance. 3. The disease usually brings a loss of 50 per cent or over of the crop. 4. The symptoms of the disease appear on all above-ground parts of the plant. A seedling blight, stem and petiole cankers, spots on leaf blades, fruit stalks and calices and a rotting of the young and mature fruit are produced. 5. The fungus may occur inside the seed. 6. The pathogene responsible for the malady is Phomopsis vexans (Sacc. & Sydow) Harter. 7. Variations of the fungus as have been observed elsewhere do not appear to occur in the fungus in Porto Rico. 8. The size of the pyenidiospores ranges from 5 to 8 microns in length to 1.3 to 3 microns in width. 9. The germ tube of a germinating spore may either enter through a stoma, enter through a wound or force its penetration through the cuticle. 10. Secondary cycles repeatedly occur in fields. 11. The fungus is capable of a saprophytic existence. 12. The prevailing temperature in Porto Rico seems adequate for spore germination. 13. Moisture is a very important factor in outbreaks of the disease. 14. The disease is probably controlled by a three- or four-years rotation. 15. Plants with the symptoms of the disease should be promptly removed from fields. 16. Although seed treatment is beneficial it never completely eliminates the pathogene. 17. Clean seed from unaffected fruit should be demanded. 18. Infested soils should be avoided in preparing seedbeds. 19. Inoculated soils can he rendered safe for seedlings if drenched with a 1-50 formaldehyde solution at the rate of one-half gallon per square foot of soil surface. An application of 4-4-50 Bordeaux mixture is highly beneficial but the formaldehyde treatment is to be preferred. The latter treatment will cost about two-thirds of one cent per seedling. 20. Bordeaux mixture (4-4-50) is quite effective in preventing seedling blight. The treatment is too expensive and therefore inapplicable under ordinary conditions. Bordeaux mixture may be of practical application where labor cost is reduced. The safest and cheapest control measure is to grow healthy seedlings and set them on in uninfested soils.

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