Hepatitis C virus-specific cellular immune responses in sustained virological responders with viral persistence in peripheral blood mononuclear cells

2013 ◽  
Vol 34 (6) ◽  
pp. e80-e88 ◽  
Author(s):  
María C. Roque-Cuéllar ◽  
Berta Sánchez ◽  
José R. García-Lozano ◽  
Juan M. Praena-Fernández ◽  
José L. Márquez-Galán ◽  
...  
Keyword(s):  
Hepatitis C Virus ◽  
Hepatitis C ◽  
Immune Responses ◽  
Peripheral Blood Mononuclear Cells ◽  
Mononuclear Cells ◽  
Viral Persistence ◽  
Peripheral Blood Mononuclear ◽  
Cellular Immune Responses ◽  
Blood Mononuclear Cells ◽  
Cellular Immune

scholarly journals Continuous release of hepatitis C virus (HCV) by peripheral blood mononuclear cells and B-lymphoblastoid cell-line cultures derived from HCV-infected patients

2005 ◽  
Vol 86 (6) ◽  
pp. 1717-1727 ◽  
Author(s):  
Patricia Baré ◽  
Ivana Massud ◽  
Cecilia Parodi ◽  
Liliana Belmonte ◽  
Gabriel García ◽  
...  
Keyword(s):  
Hepatitis C Virus ◽  
Hepatitis C ◽  
Peripheral Blood Mononuclear Cells ◽  
Peripheral Blood ◽  
Mononuclear Cells ◽  
Hcv Rna ◽  
Peripheral Blood Mononuclear ◽  
Pbmc Culture ◽  
Cell Counts ◽  
Blood Mononuclear Cells

In order to investigate hepatitis C virus (HCV) persistence and replication in peripheral blood mononuclear cells (PBMC) from a group of haemophilic individuals, HCV production and release to PBMC culture supernatants (SNs) from HCV singly infected patients and HIV/HCV co-infected patients was studied. HCV RNA+ SNs were found more frequently from HIV/HCV co-infected individuals (89·5 %) with poor reconstitution of their immune status than from singly HCV-infected patients (57 %) or from HIV/HCV co-infected individuals with a good response to highly active anti-retroviral therapy (50 %). The presence of the HCV genome in culture SNs was associated with lower CD4+ T-cell counts and with a more severe clinical picture of HIV infection. In spite of prolonged negative HCV viraemia, PBMC from HIV/HCV co-infected patients released the HCV genome after culture. HCV permissive PBMC allowed generation of HCV productive B cell lines with continuous HCV replication. These findings add further weight to the involvement of PBMCs in persistence of HCV infection and emphasize the role of B lymphocytes as HCV reservoirs.

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